Rapid identification method for African swine fever virus gene type II and non-type II and application of rapid identification method

Abstract

The invention relates to a rapid identification method for African swine fever virus gene type II and non-type II and application thereof, the 1500-site nucleotide of the specific difference site of the B646L gene is detected, the African swine fever virus gene type II is C, and the African swine fever virus gene type non-II is T. Specific primers of different genotype difference sites of viruses are designed to effectively distinguish the African swine fever virus gene type II and non-type II. The primer obtained through screening has high amplification efficiency on the African swine fever virus gene type II and low amplification efficiency on the African swine fever virus gene type non-II, the genotype of the African swine fever virus can be judged to be the type II or the type non-II without sequencing, the detection speed is high, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of virus detection, and in particular relates to a rapid identification method of African swine fever virus genotype II and non-II type and an application thereof. Background technique [0002] my country is the world's largest pork producer and consumer, with pork output accounting for about half of the world's total. African swine fever is currently one of the most important diseases causing major harm and economic losses to the world's pig industry, and is globally recognized as the "number one killer" of the pig industry. African swine fever is an acute, febrile, highly contagious zoonotic disease of pigs caused by African swine fever virus (ASFV), which is highly lethal to domestic pigs, and the mortality rate of highly pathogenic strains It can reach 100%. ASFV has strong resistance in tissues and environments. In addition, there is currently no effective vaccine and therapeutic drug. Once it spreads w...

AI Technical Summary

Problems solved by technology

African swine fever is an acute, febrile, highly contagious zoonotic disease of pigs caused by African swine fever virus (ASFV), which is highly lethal to domestic pigs, and the mortality rate of highly pathogenic strains It can reach 100%. ASFV has strong resistance in tissues and environments. In addition, there are no effective vaccines and therapeutic drugs at present. Once it spreads widely, it will seriously affect the people's livelihood in my country and even the international trade related to the pig industry.

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