Shewanella decolorationis

A technology of shewanelladecolorationis and decolorizing Shewanella, which is applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problem of the ability of Shewanella to degrade dyes that has not been found, and achieve the improvement of water ecological environment quality and simple equipment , the effect of improving efficiency

Active Publication Date: 2006-01-04
GUANGDONG INST OF MICROORGANISM
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

In recent years, new strains of this genus have been continuously discovered in the world. Most of these strains have the ability to reduce metals under anaerobic conditions. bacteria

Method used

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  • Shewanella decolorationis

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Experimental program
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Effect test

Embodiment 1

[0024] Add Na to 200 mL deionized water 2 HPO 4 .7H 2 O 2.56g, KH 2 PO 4 0.6g, NaCl 0.1g, NH 4 Cl 0.2g, Acid Scarlet GR (C.I.27290) 0.01g, sterilized at 211°C for 20 minutes as a dye culture solution. Inoculate the LB liquid medium from the slant where Shewanella depigmentation was preserved, and activate the bacteria on a shaking table at 30°C and 150r / min to make the number of bacteria reach the order of 108, and inoculate the activated bacteria solution with an inoculation amount of 1 / 10 In the dye culture solution, culture at 30°C, observe the color change of the culture solution, take 10mL of the dye culture solution every 30 minutes and centrifuge at 6000r / min to remove the bacteria, use DU640 ultraviolet spectrophotometer (BECKMAN) at 503nm Measure the absorbance value of Acid Scarlet GR (C.I.27290) in the dye culture solution, and calculate the degradation rate of the dye with the dye culture solution without adding bacteria as the control. After 2 hours, the re...

Embodiment 2

[0026] Add Na to 200 mL deionized water 2 HPO 4 .7H 2 O 2.56g, KH 2 PO 4 0.6g, NaCl 0.1g, NH 4 Cl 0.2g, Acid Scarlet GR (C.I.27290) 0.1g, sterilized at 211°C for 20 minutes as a dye culture solution. Inoculate the LB liquid medium from the slant where Shewanella depigmentation was preserved, and activate the bacteria on a shaking table at 30°C and 150r / min to make the number of bacteria reach the order of 108, and inoculate the activated bacteria solution with an inoculation amount of 1 / 10 In the dye culture solution, culture at 30°C, observe the color change of the culture solution, take 10mL of the dye culture solution every 30 minutes and centrifuge at 6000r / min to remove the bacteria, use DU640 ultraviolet spectrophotometer (BECKMAN) at 503nm Measure the absorbance value of Acid Scarlet GR (C.I.27290) in the dye culture solution, and calculate the degradation rate of the dye with the dye culture solution without adding bacteria as the control. After 4 days, the remo...

Embodiment 3

[0028] Add Na to 200 mL deionized water 2 HPO 4 .7H2 O 2.56g, KH 2 PO 4 0.6g, NaCl 0.1g, NH 4 Cl 0.2g, Reactive Brilliant Blue (C.I.612051) 0.01g, sterilized at 211°C for 20 minutes as dye culture solution. Inoculate the LB liquid medium from the slant where Shewanella depigmentation was preserved, and activate the bacteria on a shaking table at 30°C and 150r / min to make the number of bacteria reach the order of 108, and inoculate the activated bacteria solution with an inoculation amount of 1 / 10 In the dye culture solution, culture at 30°C, observe the color change of the culture solution, take 10mL of the dye culture solution every 30 minutes and centrifuge at 6000r / min to remove the bacteria, use DU640 ultraviolet spectrophotometer (BECKMAN) at 592nm Measure the absorbance of reactive brilliant blue (C.I.612051) in the dye culture solution, and use the dye culture solution without bacteria as a control to calculate the degradation rate of the dye. After 15 hours, the ...

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Abstract

The present invention relates to one new kind of Shewanella decolorationis and is Shewanella decolorationis CCTCC M 203093. By means of the separation and purification of the active sludge in the waste water treating system of Guangzhou printing and dyeing plant, one new kind of Shewanella decolorationis is obtained. The Shewanella decolorationis can degrade dye in waste printing and dyeing water effectively, raise waste water treating efficiency and improve water ecological environment quality.

Description

technical field [0001] The present invention relates to a new decolorizing Shewanella (Shewanella decolorationis) CCTCC M 203093, which has the ability to degrade dyes with broad spectrum and high efficiency. Background technique [0002] Dye molecule is a kind of artificially synthesized complex aromatic hydrocarbon compound. According to the statistics of C.I. (Color Index) in the United States, there are as many as 10,000 dyes currently in use. They not only have specific colors, but also have complex structures, low biodegradability, and most of them have three effects. These synthetic dyes are difficult to be effectively removed by conventional wastewater treatment methods, causing serious pollution to the water environment. Therefore, the treatment of dye-containing printing and dyeing wastewater and dye wastewater is one of the hot and difficult points of current research. Studying the biodegradation characteristics of chemical dyes is conducive to the correct use of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 许玫英孙国萍郭俊钟小燕曹渭岑英华
Owner GUANGDONG INST OF MICROORGANISM
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