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Process for preparing epimedium total flavone for injection

A technology of total flavonoid glycosides and crude total flavonoid glycosides, which is applied in the field of preparation of total flavonoid glycosides of Epimedium, which can solve the problem of product purity, inconsistent properties, unfavorable industrialization implementation, and cardiovascular diseases affecting the development of Epimedium total flavonoid injection Therapeutic application prospects and other issues

Inactive Publication Date: 2007-01-17
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are many types of solvents used in the current preparation process, which is not conducive to industrial implementation; the obtained extract does not meet the Pharmacopoeia injection drug standards in terms of product purity, properties and especially curative effect, so it has seriously affected the development of Epimedium total flavonoids injection and its application prospects in the treatment of cardiovascular diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Epimedium koreanum Nakai medicinal material 9.74Kg was decocted twice with 70% ethanol of 20 times the amount (weight to volume ratio g / ml, that is, 1 g medicinal material plus 20 ml of ethanol. The same below), each for 2 hours. Ethanol was recovered from the extract, concentrated into an extract with a density of about 1.2g / ml (measured at 80°C), and a total of 1.68Kg was obtained. Get 500g of extract, add 95% ethanol to make the alcohol content reach 80%, stir well and let stand for 24 hours, keep the supernatant night. Precipitate is treated twice with 80% ethanol of 2 times amount (weight to volume ratio g / ml, namely extract 500g needs to add 1000ml) in the same way. The supernatants were combined, and the recovered ethanol was concentrated into an extract with a density of about 1.05g / ml (measured at 80°C). Add ethanol to the extract to make the alcohol content reach 30%, stir well and let it stand for 24 hours, then keep the supernatant. Precipitate is treated ...

Embodiment 2

[0028] Get 500g of extract in Example 1, add 80% ethanol (tested alcohol content is 80%) of 10 times the amount (weight to volume ratio g / ml, the same below), stir well and let stand for 24 hours, keep the above Clear night. The precipitate was then treated twice with 2 times the amount of 80% ethanol in the same way. The supernatants were combined, and the recovered ethanol was concentrated into an extract with a density of about 1.15 g / ml (measured at 80°C). Add 10 times the amount of 30% ethanol (the alcohol content is 30% at this time) to the extract, stir well and let it stand for 24 hours, then leave the supernatant at night. The precipitate was then treated twice with 2 times the amount of 30% ethanol in the same way. Combine the supernatants, recover ethanol to an alcohol-free aqueous solution, and take the treated aqueous solution on a D101 macroporous resin column for adsorption. After the resin column was fully washed with deionized water, it was eluted with 30% ...

Embodiment 3

[0031]Epimedium medical material 1.02Kg, decoct twice with 50% ethanol of 20 times the amount, each time 1.5 hours. Ethanol was recovered from the extract, concentrated into a thick extract with a density of about 1.15g / ml (measured at 80°C), and a total of 176g was obtained. Add 95% ethanol to the extract to make the alcohol content reach 85%, stir well and let it stand for 24 hours, and keep the supernatant night. The precipitate was then treated twice with 2 times the amount of 85% ethanol in the same way. The supernatants were combined, and the recovered ethanol was concentrated into an extract with a density of about 1.15 g / ml (measured at 80°C). Add ethanol to the extract to make the alcohol content reach 20%, stir well and let it stand for 24 hours, then keep the supernatant. The precipitate was then treated twice with 2 times the amount of 20% ethanol in the same way. Combine the supernatants, recover the ethanol to an alcohol-free aqueous solution, and take the tre...

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Abstract

The invention relates to a process for preparing epimedium total flavone for injection for prevention and treatment of cardiovascular diseases. Epimedium drugs are refluxed and extracted by ethanol, after recovering extracting solution and condensing ethanol, the extracted liquid passes through the macroporous resin column for adsorption, is washed by water, and gradiently eluted by ethanol with different concentrations, condensed, and dried to epimedium total flavone with content over 80%.

Description

technical field [0001] The invention relates to a preparation method of total flavonoid glycosides of Epimedium, especially a preparation method of total flavonoid glycosides of Epimedium for injection. Background technique [0002] Epimedium is a plant of the genus Epimedium of Berberidaceae (Berberidaceaceae), and there are more than 40 species in the world. There are twenty-three species and four varieties in China. The 2000 edition of the Pharmacopoeia of the People's Republic of China recorded Epimedium brevicormum Maxim., Epimedium sagittatum Maxim., Epimedium pubescens Maxim., Epimedium wushanii ( Epimedium wushanense T.S.), Korean Epimedium (Epimedium koreanum Nakai) five kinds of Epimedium, and its aerial parts are used as medicinal parts. It has the effects of invigorating kidney yang, strengthening muscles and bones, and expelling rheumatism. For impotence and nocturnal emission, flaccidity of muscles and bones, rheumatic arthralgia, numbness and spasm; menopau...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07H17/07C07H1/08A61K36/296A61P9/10A61P9/12A61P15/10A61P29/00
Inventor 孔令义童玉玺袁杰
Owner CHINA PHARM UNIV
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