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Monocloned antibody for eight plant viruses and inspection thereof

A monoclonal antibody, plant virus technology, applied in anti-plant immunoglobulins, measuring devices, instruments, etc., can solve the problem of difficulty in serum preparation, and achieve the effects of wide application prospects, convenient application, and fast color reaction.

Inactive Publication Date: 2003-06-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Serum preparation is difficult due to the low concentration of such viruses in tissues

Method used

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Embodiment Construction

[0036] The invention utilizes monoclonal technology to obtain hybridoma cell lines secreting specific antibodies of BBWV2 and BBWV1, and establishes a TAS-ELISA detection system and an ACP-ELISA detection system. The detection system has higher sensitivity and specificity. It can detect BBWV2 isolates, but cannot react with BBWV1 isolates, and has high specificity. The monoclonal antibody of BBWV1 can only react specifically with the isolate of BBWV1, but does not react with the isolate of BBWV2. The invention is applied to detect BBWV isolates in different regions of my country, and it is found that the domestically distributed BBWV is BBWV2, which widely exists in crops and is one of the main pathogens on leguminous crops. But it is not yet clear whether BBWV1 exists in my country. The invention helps to understand the occurrence and strain distribution of BBWV in China, provides a technical basis for rapid and accurate identification of BBWV, and provides strong evidence f...

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PUM

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Abstract

The 19 monoclonal antibody strains of 8 monoclonal antibody for 8 plant viruses including broad bean wilt virus, cucumber mosaic virus, tomato mosaic virus, cane mosaic virus, etc are disclosed. The high-specificity high-sensitivity ACP-ELISA and TAS-ELISA methods for detecting relative viruses are disclosed on the basis of said 8 antibodies.

Description

technical field [0001] The invention relates to monoclonal antibodies of eight plant viruses and a detection method thereof. Background technique [0002] As a major pathogen infecting plants, plant viruses cause diseases of crops, fruit trees, flowers, pastures, and medicinal plants all over the world, resulting in a decline in yield and quality, and even destructive damage, which greatly affects farmers. income. The invention can specifically and sensitively detect eight kinds of plant viruses, and provide materials for disease-resistant breeding, occurrence and distribution of diseases, virus quarantine, diagnosis of virus diseases, establishment and identification of virus-free seedlings, and research of plant virus diseases and technical support. [0003] Broad bean wilt virus (BBWV) was first isolated from broad bean by Stubbs in Australia in 1947. It is a typical member of the fabavirus genus of the family Vignamoviridae and occurs widely i...

Claims

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Application Information

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IPC IPC(8): C07K16/16G01N33/577
Inventor 周雪平吴建祥陈正贤胡东维于翠青玲蒋军喜
Owner ZHEJIANG UNIV
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