Dermatan sulfate with low molecule and its preparing method

A dermatan sulfate, low-molecular technology, applied in the field of biomedicine, can solve the problems of low bioavailability, low bioavailability, complex process, etc., and achieve the goal of increasing the concentration of hydrogen peroxide, high bioavailability, and increasing the reaction temperature Effect

Inactive Publication Date: 2003-10-01
SHANDONG UNIV
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are two major pharmacokinetic issues with dermatan sulfate: one is the short half-life and the other is low bioavailability when administered by subcutaneous and intramuscular routes
As the molecular weight of dermatan sulfate increases, its bioavailability decreases
Mascellani et al once reported the preparation of a low-molecular-weight dermatan sulfate in the U.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] A best example of the practice of the present invention is given below.

[0013] The key to the preparation of low-molecular-weight dermatan sulfate is to control the molecular weight. This method adopts the hydrogen peroxide degradation method to obtain low-molecular-weight dermatan sulfate with a controllable degree of degradation.

[0014] (1) Preparation of low-molecular dermatan sulfate: make 1.0% solution of dermatan sulfate fine product with distilled water, adjust pH to 4-5, add 30% hydrogen peroxide dropwise to make the final concentration reach 2%, place in 70°C water bath Stir for 15 hours, adjust the pH to 7 with sodium hydroxide solution, and precipitate with 3 times the amount of methanol;

[0015] (2) Purification of low-molecular dermatan sulfate: the precipitate in step (1) is made into a 0.5% solution, and the membrane ultrafiltration with a molecular cut-off value of 8kD is used to collect the permeate, which is concentrated with a rotary evaporator a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A low-molecular (3000-8000) dermatan sulfate is prepared from the dermatan sulfate through degradation by hydrogen peroxide, ultrafilter, and gel chromatography. Its advantages are sure thrombus-resistant and thrombolytic functions, easy absorption, long semi-life and high biologic utilization rate.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a low-molecular dermatan sulfate and a preparation method thereof. Background technique [0002] Dermatan sulfate is a kind of complex structure and sulfated glycosaminoglycans. The molecular weight of natural products is generally 15kD ~ 5kD, and it is widely found in animal tissues. Studies at home and abroad have shown that the anticoagulant activity of dermatan sulfate is lower than that of unfractionated heparin, but its antithrombotic activity is significantly higher than that of unfractionated heparin, and its bleeding side effects are significantly smaller than that of unfractionated heparin. . However, there are two major pharmacokinetic problems with dermatan sulfate: one is the short half-life, and the other is low bioavailability when administered by subcutaneous and intramuscular routes. As the molecular weight of dermatan sulfate increases, its bioavailabili...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61P7/02C08B37/08
Inventor 姬胜利王凤山迟延青谢继青
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap