Method for quantitative determination of biochemical substance by enzyme analysis via predicting background

A technology for the quantitative determination of biochemical substances, applied in the field of clinical testing, can solve the problems of many interference factors, high cost, long reaction time, etc., and achieve the effect of high analysis efficiency, low cost and high accuracy
CN1488762AInactive Publication Date: 2004-04-14廖飞
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
廖飞
Publication Date
2004-04-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The method for quantitative determination of biochemical substance by means of predetermining background and making enzyme analysis includes the following steps: adding buffer solution and tool enzyme in the sample solution to be determined, measuring the change of light absorption with reaction time, and utilizing integral velocity equation to fit and analyze the negative curve of light adsorption consumed by substrate to predetermine background instead of directly detemining background. It can directly determine body fluid sample, and it has no meed of diluting said sample, and its determination accuracy is high.
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Description

technical field

[0001] The invention relates to a method for determining the content of specific biochemical substances in body fluids or other mixtures in the field of clinical testing, especially a method for quantitatively determining biochemical substances through enzymatic analysis by predicting the background. Background technique

[0002] Enzymatic analysis has high selectivity in determining the content of specific substances, and is of great value in the determination of specific biochemical substances in mixtures. There are two main types of enzymatic analysis methods for determining the content of specific biochemical substances in mixtures: kinetic methods and end-point equilibrium methods. The end-point equilibrium method requires high concentrations of analytical tool enzymes and a long reaction time to ensure that the substrate is completely converted into the product, and then the changes in the corresponding detection indicators are m...

Claims

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