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N-end polyclonal antibody PAPC1 of anti-human multicapsular protein-1

A technology of polyclonal antibody and polycystic protein, which is applied in anti-animal/human immunoglobulin, measuring devices, instruments, etc., can solve the problems of poor experimental stability and achieve good stability, high sensitivity, and strong specificity Effect

Inactive Publication Date: 2004-09-08
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Benefits of technology

This patented new type of protein called people's own genetic material (PCA) was discovered by researchers working with different types of cells or tissues from humans. It had many important qualities for use during these tests such as being stable when exposed to various chemical agents like histamine, trypsin, etc., having very low amounts compared to others found naturally occurring proteins, making them ideal targets for diagnostic purposes. These properties make this discovery possible because they were able to attach itself to certain parts of DNA on chromatin structure inside cell nuclei without affecting their function.

Problems solved by technology

This patented technical problem addressed by this patents relates to finding better ways to prevent autism caused by inherited nephropathy syndrome type 2 (autozy spurious polymorphisms associated diseases - ANPHVD).

Method used

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  • N-end polyclonal antibody PAPC1 of anti-human multicapsular protein-1
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  • N-end polyclonal antibody PAPC1 of anti-human multicapsular protein-1

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Embodiment Construction

[0028] The preparation of the anti-human PC-1 polyclonal antibody PAPC1 will now be described in detail.

[0029] 1. Materials

[0030] (1) Plasmids and strains: fusion protein expression vector pQE30, which contains PT5 promoter, 2 lac inducible sequences, nucleotide sequence encoding RBS, initiation codon ATG, encoding 6 consecutive histidines from upstream to downstream Nucleotide sequence of acid, multiple cloning site MCS and stop codon. The plasmid also contains the ampicillin resistance marker gene and the replicon Col E1; the expression host strain M15 contains the repression plasmid pREP4, which contains the kanamycin resistance marker gene. pQE30 and M15 were purchased from Qiagen, Germany.

[0031] (2) Animals: female New Zealand white rabbits were purchased from the Experimental Animal Center of Shanghai Second Medical University.

[0032] (3) Main reagents: total RNA extraction kit and gel recovery kit are from Huashun Company, and One-Step RT-PCR kit and Ni-NT...

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Abstract

The present invention relates to medicine molecular biology and immulogical detection technology, and is one kind of 1N-end polyclonal antibody PAPC1 of anti-human polycystic protein-1. According to cDNA sequence of human polycystic kidney disease-1 gene and the molecular structure of its encoded product, the gene is cloned to cDNA encoding PC-1 and its prokaryotic expression carrier is constituted by means of molecular biology technology. N-end fusion protein of PC-1 is induced in colibacillus and used as antigen for immunizing animal to prepare antiserum and is purified to obtain polyclonal antibody PAPC1. Comprehensive performance test shows that the antibody has stable performance, high sensitivity and powerful specificity in immunohistochemistry, enzyme-linked immunosorbent assay, immunoblotting and immunoprecipiation experiments, so that it may be used in preparing reagent for polycystic kidney disease mechanism research.

Description

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Claims

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Application Information

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Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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