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Quick seedling growing tissue culture method of Baoshan violet

A technology of Baoshan Viola and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of different cultivation and breeding conditions, achieve optimal repair of heavy metal pollution in soil or water, and increase reproduction coefficient , the effect of mass cultivation

Inactive Publication Date: 2004-12-15
SUN YAT SEN UNIV
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  • Abstract
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Problems solved by technology

[0006] Rapid seedling cultivation by tissue culture has solved the breeding problems of many other precious species, but the cultivation and breeding conditions of each species are different, requiring multiple screening tests. Seedling method

Method used

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  • Quick seedling growing tissue culture method of Baoshan violet
  • Quick seedling growing tissue culture method of Baoshan violet
  • Quick seedling growing tissue culture method of Baoshan violet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take the petiole of wild Baoshan Viola as the explant, wash it with running water, cut it into 0.5cm 2 Small pieces of 0.1% HgCl 2 Soak in water for 10 minutes, then rinse with sterile water 4 to 5 times, and inoculate on the MS basic medium of 1mg / L 6-BA+1mg / L NAA. . The culture conditions after inoculation to before hardening are: 22-25°C, 12 hours of light. One week after inoculation adventitious shoots were formed (eg image 3 ), when the height of the adventitious buds is about 2 cm, transfer them to 1 / 2MS medium to promote their root growth. After 2 weeks of rooting and cultivation, open the bottle cap to harden the seedlings, move them into the cultivation soil, and cover them with plastic bags to maintain a certain humidity. Remove and water daily. The survival rate is greater than 80%. After 4 to 5 weeks after being moved into the culture soil, the tissue-cultured seedlings have the same shape as the wild seedlings, grow robustly, open hidden flowers, set ...

Embodiment 2

[0032] Take the leaves of wild Baoshan Viola as explants, wash them with running water, and cut them into 0.5cm 2 Small pieces of 0.1% HgCl 2 Soak in water for 10 minutes, then wash 5 times with sterile water, inoculate in hormone ratio of 1mg / L KT+1mg / L 2,4D, white callus will be formed 3 days after inoculation, and obvious callus will be formed after 3 weeks. Injured tissue (such as figure 2 ). The callus was inoculated on MS medium with a ratio of 1mg / L 6-BA+1mg / L NAA, and adventitious shoots formed within 1 to 2 weeks. The culture conditions after inoculation to before hardening are: 22-25°C, 14 hours of light. When the resulting adventitious buds are about 2 cm high, transfer them to 1 / 2 MS medium to promote their root growth. After rooting and culturing for 3 weeks, open the bottle cap to harden the seedlings, spray a little water, cultivate them in a sterile room for 2 days, and then transfer them to the greenhouse for cultivation. After 1 day, remove the seedlings...

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Abstract

The fast seedling growing process includes selecting leaf or leafstalk of Viola baoshanensis as explant, disinfection, inoculating onto MS inducing culture medium with hormone 0.5-1.0 mg / L 6-BA plus 0.5-1.5 mg / L NAA or 0.5-1 mg / L KT plus 1-1.5 mg / L 2, 4-D to induce the growth of adventitious bud and callus, rooting culture and seedling strengthening, and transplantation to obtain tissue culture seedling. The grown seedling of Viola baoshanensis can complete its life history in culture medium and soil, and has high transplantation survival rate, domestication period of 2-3 months and high propagation coefficient. The said method makes it possible to culture Viola baoshanensis as one kind of super enriched heavy metal plant.

Description

technical field [0001] The invention relates to a method for rapidly raising seedlings of the heavy metal super-accumulating plant Viola baoshanensis through tissue culture. Background technique [0002] Baoshan Viola (Viola baoshanensis) is a new species of Violaceae, and it is also a newly discovered heavy metal hyperaccumulator plant in my country. The potential value of Baoshan Viola in scientific research and remediation of water or soil heavy metal pollution has attracted the attention of people in the scientific, environmental and business circles. [0003] At present, the research and experiment on Baoshan Viola requires a large number of seedlings, and these seedlings are basically directly collected in the Baoshan mining area of ​​Guiyang County, Hunan Province. Field surveys show that Baoshan Viola is only locally concentrated within two square kilometers of the Baoshan mining area, and has not been found in other mining areas in my country. Previous and ongoing...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 邓冬梅束文圣廖斌蓝崇钰陈同斌张军彭光天
Owner SUN YAT SEN UNIV
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