Termite resisting method utilizing metabolic product of tree endophyte to inhibit symbiotic bacteria of termite

A metabolite inhibition, metabolite technology, applied in the field of termite resistance, can solve environmental pollution and other problems

Inactive Publication Date: 2005-04-27
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Existing medicaments for killing termites are all chemical medicaments, so they all have great toxic and side effects, causing serious environmental pollution

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0004] Specific embodiment one: this embodiment utilizes the metabolites of endophytic bacteria in trees to inhibit termite symbiotic bacteria so as to realize the anti-termite method: (1) separate symbiotic bacteria with lignin and / or cellulose decomposition ability from termites as indicator bacteria; (2) isolate tree endophytes from termite-resistant trees, and ferment them to obtain tree endophyte metabolites; (3) use Oxford cup method to inhibit termite symbiotic bacteria by using tree endophyte metabolites Bacteria test; (4) Soak the filter paper sheet with metabolites of tree endophytic bacteria that have an inhibitory effect on the symbiotic bacteria of termites, and carry out a feeding test on termites. The termite-resistant trees are Western Red Cedar and port-orford-cedar.

specific Embodiment approach 2

[0005] Specific embodiment two: the present embodiment utilizes the metabolites of tree endophytes to suppress termite symbiotic bacteria so as to realize the anti-termite method:

[0006] 1. Selection of materials:

[0007] 1. Western Red Cedar (sampled in Louisiana, USA on September 10, 2003)

[0008] 2. port-orford-cedar (sampled in Oregon, USA in September 2003)

[0009] 3. Black-winged soil termite (Odontotermes formosanus) (sampled from Mount Luojia, Wuhan, China)

[0010] 4. American termites (sampling from Louisiana State University, USA)

[0011] Second, the preparation of culture medium:

[0012] (1) Indicator culture medium:

[0013] 1. Cellulose medium:

[0014] 1.1 Bacterial cellulose medium:

[0015] CMC-Na (sodium carboxymethylcellulose referred to as CMC) 10g, NH 4 h 2 PO 4 0.5g, K 2 HPO 4 2.0g, MgSO 4 2.0g, MnSO 4 ·H 2 O2.5mg, FeSO 4 ·7H 2 O7.5mg, NaCl0.3g, peptone 10g, agar 20g, water 1000ml.

[0016] 1.2 Fungal cellulose medium:

[0017] CM...

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Abstract

The present invention is termite resisting method of utilizing metabolic product of tree endophyte to inhibit symbiotic bacteria of termite. The present invention includes separating symbiotic bacteria with lignin and/or cellulose decomposing capability from termite body as the indication bacteria; separating tree endophyte from termite tree and fermenting to obtain metabolic product of tree endophyte; performing bacteriostasis test with the metabolic product of tree endophyte on symbiotic bacteria via Oxford process; and soaking filter paper with the tree endophyte with bacteriostasis on the symbiotic bacteria and feeding termite with the soaked test paper. Statistics shows that the termites fed with the filter paper has obviously higher death rate and obviously lower activity, and this probes that the fermented liquid contains termite resisting compound.

Description

Technical field: [0001] The invention relates to a method for isolating endophytes, in particular to an anti-termite method for inhibiting termite symbiotic bacteria by using metabolites of tree endophytes. Background technique: [0002] Existing medicaments for killing termites are all chemical medicaments, so they all have very large toxic and side effects and cause serious environmental pollution. Invention content: [0003] The object of the present invention is to provide an anti-termite method that utilizes metabolites of tree endophytic bacteria to inhibit termite symbiotic bacteria, and the method uses microorganisms to produce non-toxic or low-toxic compounds that can resist termites. The present invention utilizes the metabolites of tree endophytes to inhibit termite symbiotic bacteria so as to realize the anti-termite method: (1) isolate symbiotic bacteria with lignin and / or cellulose decomposition ability from termites as indicator bacteria; (2) Isolate tree e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00A01G23/00
Inventor 周东坡平文祥许中允李强刘军周刚韩雅红苏泊
Owner HEILONGJIANG UNIV
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