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Use of active extracts to lighten skin, lips, hair and/or nails

A technology of extracts, skins, applied in the field of topical compositions

Inactive Publication Date: 2005-11-09
AVON PROD INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] To date, these extracts have not been used as active ingredients in compositions for brightening skin, lips, hair or nails

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The melanosome uptake test (Swollen fern and Limonium) was carried out in the following manner. Confluent cultured B16 melanocytes produce moderate amounts of melanosomes. However, to induce increased melanosome production in this cell line, half-confluent (60%) cultured B16 cells were treated for approximately 36 hours with normal growth medium containing 10 mM ammonium chloride (final concentration). The medium was then aspirated and the hypermelanized cells (2 x 2 ml) washed with distilled water to provide a hypotonic pressure to the cells. An aliquot (2 ml) of hypotonic lysis solution (0.02% NP-40 in water) was added to each plate and incubated for approximately 5 minutes at room temperature. Cell lysates were then examined by light microscopy and cell material from the 3 culture plates was pooled into a 15 ml conical tube and centrifuged (200 xg) for 5 min to remove cell debris. The resulting melanosome-containing supernatant was transferred to a clean 15 ml coni...

Embodiment 2

[0056] In the B16 assay (Limonium), the active ingredient is tested in B16 murine melanoma cells cultured in a monolayer. These cells constitutively produce melanin and are a model system for testing inhibition of melanin synthesis. cells in 5 x 10 3 Cells / well were inserted into 96-well plates and cultured for 24 hours. The original medium was then replaced with fresh medium containing plant extracts. Each active ingredient was added to 6 wells of B16 cells on a 96-well plate for statistical analysis. Cells were treated with medium alone as a negative control, or with the substance to be tested for 7 days. Plates were read at 540 nM to detect melanin formation. The increase in absorbance at 540 nM reflects higher melanin content in the wells.

[0057] At 0.05% (weight / volume), Limonium Limonium showed a 61% reduction in pigmentation compared to the positive control.

Embodiment 3

[0059] exist 14 In the C-dopa incorporation experiment (buta rivet), when 14 Melanogenic activity was determined by measuring the radioactive melanin formed when C-dopa was converted to the acid-insoluble melanin biopolymer in B16F10 melanoma cells. cells in 2 x 10 4 The density of cells / well was inserted into 24-well plates and cultured for 48 hours. Then with plant extracts and 0.2 μCi 14 Fresh medium of C-dopa was used instead of the original medium. Cells were further incubated for 24 hours. After incubation, the medium was discarded and the cells were rinsed with PBS, lysed by adding 0.125 ml 1N NaOH and incubated at 37°C for 30 minutes, then neutralized with 0.025 ml 5N HCl. The resulting cell lysate was transferred to a liquid scintillation vial and mixed with a scintillation mixture, and the radioactivity was measured by a scintillation counter. A portion of the cell lysate was saved and protein content was measured by Lowry's method. Results were normalized to ...

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PUM

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Abstract

There is provided a composition having at least one of the following active extracts Butea frondosa, Naringi crenulata, Stenoloma chusana, or any combinations thereof. There is also provided a composition having at least one of the following additional extracts Azadirachta indica, Glycyrrhiza glabra linn., Morinda citrifolia, tomato glycolipid or any combinations thereof in combination with one or more of the active extracts. The compositions and methods of the invention are effective to improve the aesthetic appearance of skin, lips, hair and / or nails, especially by lightening the skin, lips, hair and / or nails.

Description

[0001] related application [0002] This application is a continuation-in-part of co-pending US Patent Application No. 10 / 321,706, filed December 17, 2002, and claims priority and benefits thereof. technical field [0003] The present invention relates to topical compositions containing natural or synthetic active ingredients derived from plants or plant materials. In particular, the present invention relates to topical compositions for improving the appearance of the skin, lips, hair and / or nails, in particular by making the skin, lips, hair and / or nails shiny. More specifically, the present invention relates to bioactive extracts that enhance the aesthetics of the skin, lips, hair and / or nails, in particular by giving them a shine. Background technique [0004] Consumers are always looking for ways to improve the appearance of their hair, skin, lips and nails. There is a demand for products that effectively brighten and reduce hyperpigmentation in hair, skin, lips and na...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61K36/11A61K36/48A61K36/484A61K36/58A61K36/746A61K36/75A61K36/81A61Q5/00A61Q5/08A61Q19/00A61Q19/02A61Q19/08
CPCA61K36/484A61K36/48A61Q19/00A61K36/81A61K8/97A61K36/58A61Q5/08A61K36/75A61K36/746A61Q19/08A61Q19/02A61K36/11A61K8/9741A61K8/9789A61P17/00A61P29/00A61K2300/00
Inventor 哈里斯·马哈林格姆布莱恩·琼斯妮科尔·麦克凯恩
Owner AVON PROD INC
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