Culture method for ammonia-producing bacterium and its application

A culture method and culture solution technology, applied in the direction of bacteria, etc., can solve problems such as difficult protection technology, and achieve the effect of convenient and powerful corrosion mechanism and protection technology, and good ammonia production performance

Inactive Publication Date: 2005-11-30
725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, except for sulfate-reducing bacteria, people know very little about other corrosive bacteria, so it is difficult to understand the corrosion mechanism of some corrosive microorganisms and to study related protective technologies.

Method used

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  • Culture method for ammonia-producing bacterium and its application
  • Culture method for ammonia-producing bacterium and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Under laboratory conditions, the isolated preserved strain Staphylococcus asaii (Gluconobac ter asaii) 725A CCTCC NO.M204062 was used at 30°C on a slant medium (formulation: glucose 10g, beef extract 20g, peptone 5g, aged Seawater 1000ml, pH value is natural, agar 20g.) Cultivate for two days, then take a loop and inoculate on the liquid medium with an inoculation needle. The formula of the ammonia-producing bacteria liquid culture medium is: 1000ml seawater, 5g peptone, 30g beef extract, 10g glucose, pH value is 7.2, and it is sterilized under high temperature and high pressure in a sterilizing pot. Put 10% of the bacteria into the corrosion test bottle, immerse the B30 sample in the solution containing bacteria, the temperature is 22°C at room temperature, take it out after one month, remove the corrosion products on the surface of the sample, and carry out the surface electron microscope analysis to obtain the following: figure 1 Corrosion effect shown.

[0017] Dep...

Embodiment 2

[0019] Take a loop of fresh Staphylococcus asaii (Gluconobacter asaii) 725ACCTCC NO.M204062 on the slant medium with an inoculation needle and inoculate it into a liquid medium (glucose 5g, beef extract 20g, peptone 5g, aged seawater 1000ml), after cultivating for 24h, Add it into the sterilized ammonia-producing culture solution at a ratio of 15%. The formula of the ammonia-producing culture solution is as follows: 5 g of peptone, 1000 ml of aged sea water, pH 7.2. Immerse the high manganese aluminum bronze sample in the solution containing bacteria, take it out after one month, remove the corrosion products on the surface of the sample, and carry out the surface electron microscope analysis to obtain the following: figure 2 Corrosion effect shown.

[0020] Depend on figure 2 It can be seen that pitting corrosion also occurred on the surface of high manganese aluminum bronze.

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Abstract

The invention discloses a method for nurture and application of ammonia-generating bacterium, culturing bacterial strain in bevel culture medium, seeding and culturing in liquor culture medium, and then seeding and culturing in ammonia-generating culture liquid to generate ammonia. The bacterium breeds fast and generates ammonia steadily by optimizing culture medium and culture condition. Soaking copper alloy into the culture liquid containing ammonia-generating bacterium, it produces obvious corrosion, which provides convenient and potent means for microbial corrosion and prevention technique.

Description

technical field [0001] The invention relates to a new method for cultivating ammonia-producing microorganisms and its application. It belongs to the field of microbial corrosion. Background technique [0002] The consequences of microbial corrosion are quite serious, accounting for about 20% of all corrosion (A Review of Some Characteristics of MIC Caused by Sulfate-reducing Bacteria: Past, Present and Future, Anti-corrosion Methods and Materials[J], Bradford, P.173 -182). Copper and copper alloys have very good seawater corrosion resistance in clean seawater, but their corrosion rates are high in polluted or eutrophic seawater. If the content of organic matter in the corrosion site exceeds 20%, the corrosion is very likely to be caused by organisms (Licina). However, people know very little about other corrosive bacteria except sulfate-reducing bacteria, so it is difficult to understand the corrosion mechanism of some corrosive microorganisms and to study related protect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 刘光洲段东霞黄国胜王军
Owner 725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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