Natural plant pesticide, preparation method and application thereof
A natural plant and insecticide technology, applied in the field of plant pesticides, can solve the problems of pest resistance, residual toxicity environment, high residue, etc., and achieve the effect of wide distribution, mature technology and large storage capacity
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Embodiment 1
[0018] Antifeedant and repellent effects of patchouli essential oil on Trichosanthes alba and Lychee leaf roller
[0019] 1.1 Essential oil for testing: Patchouli essential oil is self-provided by this research institute, and each milliliter contains about 50g of crude drug.
[0020] 1.2 Test insects
[0021] Sandalwood butterfly (Delias aglaia L.): The newly hatched sandalwood larvae were collected from the specimen garden of our institute, put into large plastic boxes, and fed with young sandalwood leaves. When the larvae reached the fifth instar, they were try.
[0022] Homona coffearia Nietner: The newly hatched larvae of the litchi brown-banded leaf tortrix (Homona coffearia Nietner) were collected from the Zengcheng orchard, put into large plastic boxes, and fed with young litchi leaves. When the larvae reached the fifth instar, they were fed try.
[0023] 1.3 Determination of antifeedant effect
[0024] Medicament preparation: using 6% Tween-80 solution as a solvent...
Embodiment 2
[0044] Antifeedant effect of patchouli essential oil on Spodoptera litura
[0045] 1.1 Essential oil for testing: Patchouli essential oil is self-provided by this research institute, and each milliliter contains about 50g of crude drug.
[0046] 1.2 Test insects
[0047]Spodoptera litura Fab.: The egg masses of Spodoptera litura Fab. were collected from the field and placed in a moisturizing petri dish. After the eggs hatched into larvae, they were transferred to a large glass tank and fed with cassava leaves. When the larvae grew to At the 3rd instar, neat and consistent larvae were picked for testing.
[0048] 1.3 Test method
[0049] Drug preparation: using acetone as solvent, the patchouli essential oil was diluted to five gradient concentrations of 2.5mg / mL, 5mg / mL, 10mg / mL, 20mg / mL, and 40mg / mL respectively.
[0050] The determination of antifeedant activity for selective feeding and antifeedant activity for non-selective feeding are the same as in Example 1.
[0051...
Embodiment 3
[0058] Insecticidal effect of patchouli ketone and patchouli essential oil
[0059] 1.1 Test agent: Patchouli essential oil is self-extracted by this research institute, and each mL contains about 50 g of crude drug; patchouli ketone is separated and prepared from this essential oil by our research laboratory, with a yield of 0.25% and a purity of more than 98.5%. Using 6% Tween-80 solution as a solvent, the patchouli essential oil was diluted to five gradient concentrations of 2.5mg / mL, 5mg / mL, 10mg / mL, 20mg / mL, and 40mg / mL; -20 solution is used as a solvent, and the patchoulinone is diluted to five gradient concentrations of 1mg / mL, 2mg / mL, 4mg / mL, 8mg / mL, and 10mg / mL.
[0060] 1.2 Tested insects
[0061] Cabbage butterfly (Preris rapae L.): The pupae and advanced larvae of the cabbage butterfly (Preris rapae L.) were collected from the teaching and practice area of the specimen garden of our institute. In the insect cage, put potted kale in the cage, and soak cotton in ...
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