Natural plant pest killing and sterilizing agent and its preparing method and use
A technology of natural plants and fungicides, applied in the field of plant pesticides, can solve the problems of pest resistance, residual toxic environment, high toxicity, etc., and achieve the effects of wide distribution, mature technology and large storage.
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[0017] Example 1
[0018] Antifeedant and repellent effects of patchouli essential oil on sandalwood butterfly and lychee leaf roller
[0019] 1.1 Essential oil to be tested: Patchouli essential oil is self-provided by this research institute, and contains about 50g crude drug per ml.
[0020] 1.2 Test insects
[0021] Delias aglaia L.: Pick up newly hatched sandalwood larvae from the specimen garden of our institute, put them in a large plastic box, and feed them with young sandalwood leaves. When the larvae reach 5th instars test.
[0022] Homona coffearia Nietner (Homona coffearia Nietner): The newly hatched larvae of Homona coffearia Nietner were collected from the Zengcheng Orchard, placed in a large plastic box, and fed with young lychee leaves, and provided when the larvae reached 5th instar test.
[0023] 1.3 Determination of antifeedant effect
[0024] Pharmaceutical preparation: Using acetone as the solvent, the patchouli essential oil was diluted to 2.5mg / L, 5mg / L, 10mg...
Example Embodiment
[0042] Example 2
[0043] Antifeedant effect of patchouli essential oil on Prodenia litura
[0044] 1.1 Essential oil to be tested: Patchouli essential oil is self-provided by this research institute, and contains about 50g crude drug per ml.
[0045] 1.2 Test insects
[0046] Spodoptera litura Fab.: The eggs of Spodoptera litura Fab. are collected from the field and placed in a moisturizing petri dish. After the eggs hatch into larvae, they are transferred to a large glass aquarium and fed with cassava leaves. When the larvae grow to Pick out neat and consistent larvae for testing at 3rd instar.
[0047] 1.3 Test method
[0048] Pharmaceutical preparation: Using acetone as the solvent, the patchouli essential oil was diluted to 2.5mg / L, 5mg / L, 10mg / L, 20mg / L, 40mg / L and five gradient concentrations.
[0049] The selective feeding antifeedant activity determination and the non-selective feeding antifeedant activity determination are the same as in Example 1.
[0050] 1.4 Non-selecti...
Example Embodiment
[0057] Example 3
[0058] Insecticidal effect of patchouli ketone and patchouli essential oil
[0059] 1.1 Test medicine: Patchouli essential oil is self-extracted by this research institute, and each mL contains about 50g crude drug; patchouli ketone is separated and prepared from this essential oil by our laboratory, with a yield of 0.25% and a purity of over 98.5%. Using acetone as the solvent, the patchouli essential oil was diluted to 2.5mg / L, 5mg / L, 10mg / L, 20mg / L, and 40mg / L. The patchouli ketone was diluted to 1mg / L. Five gradient concentrations of L, 2mg / L, 4mg / L, 8mg / L, and 10mg / L.
[0060] 1.2 Test insects
[0061] Pieris rapae (Preris rapae L.): The pupa and advanced larvae of Pieris rapae were collected from the teaching and practice area of our specimen garden. The larvae were collected in a large petri dish after being pupated, and moved into a 50cm long, 60cm wide and 80cm high larva when they emerged In the insect cage, put potted medusa in the cage, and immerse...
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