Therapeutic ultrasonic microvesicle for tumour ultrasonic therapy and its preparing method

A technology of ultrasonic microbubbles and ultrasonic therapy, applied in the field of tumor therapy, to achieve the effect of promoting thrombus formation in local tissue microcirculation

Inactive Publication Date: 2007-03-28
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no relevant literature report on the combination of coagulation factors and ultrasonic microbubbles to prepare therapeutic microbubbles at home and abroad.

Method used

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  • Therapeutic ultrasonic microvesicle for tumour ultrasonic therapy and its preparing method
  • Therapeutic ultrasonic microvesicle for tumour ultrasonic therapy and its preparing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Property Evaluation of Therapeutic Microbubbles of the Present Invention

[0029]First prepare the lipid suspension; then make the green fluorescently labeled prothrombin complex (FITC-PCC) into a 10mg / ml solution; Bubble preparation (horizontal reciprocating mechanical oscillation, working frequency ≥ 4500 times / min, vibration amplitude 15 ± 1mm, time 45s); the second group, after adding 0.5ml FITC-PCC to the lipid suspension, use fluorocarbon The gas was replaced with air, and the mechanical oscillation was carried out according to the same working parameters for 45s; in the third group, after the microbubble preparation was completed, the bottle stopper was uncovered and 0.5ml FITC-PCC was added, and the vortex mixer was shaken or shaken by hand for 1min. After the above preparation is completed, incubate at room temperature for 30 minutes, take out half and let it stand, and wash the other half by flotation method: put the contrast agent in a centrifuge tu...

Embodiment 2

[0031] Example 2 Therapeutic Embolization Experiment of Microbubbles of the Present Invention

[0032] 1. Experimental method

[0033] With 2% pentobarbital sodium 40mg / kg intraperitoneal injection, after anesthesia, the rabbit was fixed in the supine position on the experimental table, the ear vein access was established, the abdominal skin was prepared, an 8 cm incision was made in the middle abdomen along the midline of the abdomen, and Gently pull a section of ileum and mesentery out of the abdomen in the direction of the free end of the cecum, place it under a stereomicroscope, and adjust the microscope to an appropriate magnification (×90), so that it can clearly show the blood flow in the microvessels of the mesentery. Select small vessels The running mesentery area with less fat was used as the irradiation area.

[0034] 2. Experimental grouping

[0035] ① Fibrinogen microbubble treatment ultrasound group: Mix the two according to the ratio of 0.5ml / kg microbubble an...

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Abstract

The present invention relates to a therapeutic type ultrasonic microvesicle for tumor ultrasonic therapy, it includes ultrasonic contrast medium microvesicle containing gas endonucleus and blood coagulation factor, the blood coagulation factor is absorbed on the ultrasonic contrast medium microvesicle surface or the blood coagulation factor is adsorbed on the microvesicle surface and covered in its interior so as to form ultrasonic microvesicle in which the blood coagulation factor is combined. Under the proper therapeutic ultrasonic action said therapeutic type ultrasonic microvesicle can promote local tissue microcirculation thrombosis, and can be used for curing the diseases of tumor, etc.

Description

technical field [0001] The invention belongs to tumor therapeutics in medicine, and relates to a novel therapeutic ultrasonic microbubble in the field of tumor ultrasonic treatment, in particular to its composition and preparation method. Background technique [0002] With the in-depth research on microbubble ultrasound contrast agent, its potential non-invasive therapeutic effect is attracting attention, mainly in the following two aspects. First of all, various microbubbles (especially lipid membrane microbubbles, whose structure is similar to liposomes) are good drug carriers, which are easy to combine with genes, proteins or fat-soluble drugs, such as: lipid microbubbles used by Unger et al. The package carries the anti-tumor drug paclitaxel (Unger EC, et al.InvestRadiol, 1998, 33:886-892), Porter et al. use albumin microbubble PESDA to carry antisense oligonucleotides, etc. (Tsutsui JM, Cardiovascular Ultrasound 2004, 2: twenty three). There are many types of ultrason...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61K38/16A61K47/24A61K47/34A61K47/42A61K47/48A61K49/22A61P35/00A61N7/02A61K47/10
Inventor 刘政谭开彬杨莉高云华刘平李秋颖
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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