Northern aweto strain and its selection breeding method
A technology of Cordyceps militaris and Cordyceps militaris, which is applied in the field of breeding and producing high-yield and high-quality strains of Cordyceps militaris, can solve the problems of variation and loss of excellent traits of strains, and achieve the effect of avoiding the accumulation of homokaryons
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Embodiment 1
[0023] The surface of Cordyceps militaris sporocarps collected in the field or artificially cultivated with mature asthecia is sterilized, placed on top of a sterile PDA culture plate, and cultured in a culture room with a temperature of about 20-25°C until ascospores When ejected to the surface of the PDA medium and germinate and grow into tiny colonies visible to the naked eye, single-spore colonies are picked and transferred to new PDA culture plates or test tubes for cultivation; this process can be assisted by microscope observation to observe ascospore germination The process, and ensure that the picked ascospore monospore colonies.
[0024] After the obtained ascospore monospore strains were purely cultured and multiplied on the PDA medium, samples were taken to observe the characteristics of the anamorphic sporulation structure of each bacterial strain under a microscope, and the anamorphic sporulation structure was respectively cephalosporin type and Paecilomyces type...
Embodiment 2
[0027] After the fruiting bodies of Cordyceps militaris with mature asthecia collected in the field or cultivated artificially are surface-sterilized, they are placed on top of a sterile culture plate poured with PDA-modified medium, and placed in a culture room with a temperature of about 20-25°C. Medium culture, when the ascospores are projected onto the surface of the PDA-improved medium and germinate and grow into tiny colonies visible to the naked eye, respectively pick single-spore colonies and transfer them to new culture plates or test tubes of the PDA-improved medium for future use; this process Observation can be assisted by a microscope to observe the process of ascospore germination, and to ensure that the ascospore monospore colony is picked. The formula of the PDA modified medium used is: 200 grams of potatoes, 20 grams of sucrose, 3 grams of peptone, 3 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, 10 milligrams of vitamin B1, and 1000 m...
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