Pediculicidal and ovacidal treatment compositions and methods for killing head lice and their eggs
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Examples
example 1
Testing of Spinosyn A and Spinosyn D on Pediculis aptitis Eggs
[0055] Both Spinosyn A and Spinosyn D are sold together as the active ingredient for Conserve SC.
[0056] Conserve SC is a commercial product from DowElanco used for insect control of turf and ornamental plants. The combination of Spinosyn A and Spinosyn D is known as Spinosad. Conserve SC contains 11.60%, by weight, of the Spinosad. Spinosad contains approximately 80% Spinosyn A and 20% Spinosyn D. Three dilutions of the Conserve SC were created:
[0057] Dilution 1 contained 8.62% Conserve SC, 15% isopropanol, 10% propylene carbonate, and 53.45% deionized water. Dilution 1 resulted in a 1% Spinosad concentration.
[0058] Dilution 2 contained 21.55% Conserve SC, 15% isopropanol, 10% propylene carbonate, and 66.38% deionized water. Dilution 2 resulted in a 2.5% Spinosad concentration.
[0059] Dilution 3 contained 43.10% Conserve SC, 15% isopropanol, 10% propylene carbonate, and 31.9% deionized water. Dilution 3 resulted in a ...
example 2
In-vitro Ovacidal Testing
[0067] In vitro tests were conducted on human head lice with six formulations in order to study the pediculicidal and ovacidal activity of the formulations. No human subjects were used. The ovacidal results were as follows:
TABLE 3ActiveSingle ExposureDouble ExposureDescriptionIngredient %Ovacidal RateOvacidal RateCream Rinse A1% Spinosad80%100% Cream Rinse A12% Spinosad95%100% Cream Rinse B1% Spinosad85%84%Cream Rinse C1% Spinosad76%94%Nix ® (control)1% Permethrin76%58%Deionized Water11% 8%
Note that the 76% ovacidal rate for Nix ® after single exposure is an outlier versus the prior testing which was usually 50-60% ovacidal rate after single exposure.
[0068]
TABLE 4CreamCreamCreamCreamTrade NameINCI NameRinse ARinse A1Rinse BRinse CDeionized44.844.2544.8244.81WaterStabilezePVM / MA1.11.11.11.1QMDecadieneCrosspolymerPropylenePropylene3333GlycolGlycolCetostearylCetearyl3333Alcohol NFalcoholProcol-Ceteareth-200.90.90.90.9CS-20Ammonyx 4Stearalkonium4.174.174.174...
example 3
In-Vitro Pediculicidal Testing
[0071] The following are results taken for the same formulations as Example 3 as tested on adult lice for the pediculicidal rates.
TABLE 5Single ExposureSingle Exposure1-Minute Immersion10-Minute ImmersionActive%%% Alive%% AliveFormulationIngredient %DeadMoribund& Well% DeadMoribund& WellA20.5%77%23%—100%——SpinosadA 1%92% 8%—100%——SpinosadA1 2%82%18%—100%——SpinosadB 1%92% 8%— 97%3%—SpinosadC 1%87%13%—100%——SpinosadNix ® 1%92% 8%— 92%8%—PermethrinDeionized— 3%—97% 2%—98%Water
[0072] All measurements on this chart were taken at 4 hours after exposure. Moribund means that the lice were in terminal stages of life, from which they can never recover any useful function. In this phase, the lice may show signs of central nervous system or peripheral nerve toxicity, such as tremors, convulsions or twitching of a leg or antenna.
[0073] From this study, one can conclude that 0.5%, 1% and 2% Spinosad deliver excellent pediculicidal activity at 1-minute and 10...
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