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A kind of technology of extracting butenyl spinosad from fermentation liquid of Saccharopolyspora spp.

A technology for Saccharopolyspora chinensis and bactericide, which is applied in the field of extracting butenyl spinosad, and can solve the problem that the content of butenyl spinosad D cannot be controlled, the content of butenyl spinosad is low, and separation and extraction are difficult. problems, to achieve the effect of controllable product composition, saving dosage and energy consumption, and improving filtration efficiency

Active Publication Date: 2021-06-25
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the research on spinosyns in the world is still in its infancy. The content of spinosyns in the fermentation broth is low, which makes separation and extraction difficult, and it is impossible to control the spinosyns A and butyl in the product. The content of spinosyn D, its application is very limited

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  • A kind of technology of extracting butenyl spinosad from fermentation liquid of Saccharopolyspora spp.
  • A kind of technology of extracting butenyl spinosad from fermentation liquid of Saccharopolyspora spp.

Examples

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Embodiment 1

[0037] A kind of technique of extracting butenyl spinosyn from Saccharopolyspora barbae fermented liquid, concrete steps are as follows:

[0038] (1) After fermentation, before putting into the tank, add sodium hydroxide solution with a concentration of 0.1-0.2g / mL to 100L fermentation broth, adjust the pH of the fermentation broth to 8.5, then raise the temperature, and add diatomaceous earth and perlite to filter in turn After adding diatomite and perlite, the concentration of diatomite and perlite is 0.015g / mL and 0.025g / mL respectively, mix evenly, heat up to 90°C, keep stirring for 1.5h, a large amount of flocculent suspensions are formed in the fermentation broth, and the pre- Treated fermented liquid; after pretreatment, the fermented liquid is filtered by a plate and frame filter press or a centrifuge, and the time required to complete solid-liquid separation is 1h. The content of butenyl spinosyn in the filtrate is 50 μg / mL, and the filter cake is collected; The colle...

Embodiment 2

[0048] A kind of technique of extracting butenyl spinosyn from Saccharopolyspora barbae fermented liquid, concrete steps are as follows:

[0049] (1) After fermentation, before putting into the tank, add sodium hydroxide solution with a concentration of 0.1-0.2g / mL to the 100L fermentation broth, adjust the pH of the fermentation broth to 9.5, then raise the temperature, and add diatomaceous earth and perlite to filter in turn After adding diatomite and perlite, the concentration of diatomite and perlite is 0.025g / mL and 0.035g / mL respectively, mix evenly, heat up to 95°C, keep stirring for 2.5h, a large amount of flocculent suspensions are formed in the fermentation broth, and the pre- Treated fermented liquid; after pretreatment, the fermented liquid is filtered by plate and frame filter press or centrifuge, the time required to complete solid-liquid separation is 40min, the content of butenyl spinosyn in the filtrate is 30 μg / mL, and the filter cake is collected; The collec...

Embodiment 3

[0057] A kind of technique of extracting butenyl spinosyn from Saccharopolyspora barbae fermented liquid, concrete steps are as follows:

[0058] (1) After fermentation, before putting into the tank, add sodium hydroxide solution with a concentration of 0.2-0.3g / mL to the 100L fermentation broth, adjust the pH of the fermentation broth to 9.0, then raise the temperature, and then add diatomaceous earth and perlite to aid filter After adding diatomite and perlite, the concentration of diatomite and perlite is 0.02g / mL and 0.04g / mL respectively, mix evenly, heat up to 95°C, keep stirring for 3 hours, a large amount of flocculent suspensions are formed in the fermentation broth, and pretreatment post-fermentation broth; after pretreatment, the fermentation broth is filtered with a plate-and-frame filter press or centrifuge, the time required to complete solid-liquid separation is 30min, and the content of butenyl spinosyn in the filtrate is 20 μg / mL, and the filter cake is collect...

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Abstract

The invention discloses a process for extracting butenyl spinocidin from fermentation broth of Saccharopolyspora spp., which includes the steps of pretreatment of fermentation broth, soaking and extraction of dried bacteria powder, concentration, extraction, crystallization, etc., and obtains a method with a purity greater than 90 % of butenyl spinosyns. The crystallization process combines elution crystallization and low-temperature crystallization, while ensuring the yield of recrystallization, the content of butenyl spinosyn A and butenyl spinosyn D in the butenyl spinosyn product can be controlled. control, the former is 5‑8 times the content of the latter. The process has the advantages of low cost, simple production equipment, high yield and controllable product composition, and has industrial application prospects.

Description

technical field [0001] The invention relates to a method for extracting antibiotics from fermentation broth. More specifically, it relates to a process for extracting butenyl spinosyn from the fermentation broth of Saccharopolyspora. Background technique [0002] Butenyl-spinosyns (butenyl-spinosyns), also known as pogonins, are a class of macrocyclic endosyns with similar structures and the same insecticidal mechanism produced by the soil actinomycete Saccharopolyspora pogona. Esters insecticides. Butenyl spinosyn has the unique insecticidal mechanism of spinosad, and has strong feeding toxicity and rapid contact toxicity to pests. It mainly acts on nicotinic acetylcholine receptor (nAchR) and γ-aminobutyric acid receptor (γ-amino-hutyric acid receptor, GABA), and stimulates the nervous system to cause excitement, resulting in non-functional muscle contraction, Trembling, exhaustion and paralysis, etc., will eventually cause the death of pests. The unique insecticidal me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/06C07H17/08
CPCC07H1/06C07H17/08
Inventor 王超郭超郭伟群常晓娇孙长坡
Owner ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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