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Process for extracting butenyl-spinosyn from fermentation liquor of Saccharopolyspora pogona

A technology of Saccharopolyspora spp. and fungcidins, which is applied in the field of extraction of butenyl pleocidins, and can solve the problems of uncontrollable butenyl pleocidin D content, low butenyl pleocidin content, separation and extraction difficulties, etc. problems, to achieve the effects of controllable product composition, saving dosage and energy consumption, and low process cost

Active Publication Date: 2020-02-11
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the research on spinosyns in the world is still in its infancy. The content of spinosyns in the fermentation broth is low, which makes separation and extraction difficult, and it is impossible to control the spinosyns A and butyl in the product. The content of spinosyn D, its application is very limited

Method used

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  • Process for extracting butenyl-spinosyn from fermentation liquor of Saccharopolyspora pogona
  • Process for extracting butenyl-spinosyn from fermentation liquor of Saccharopolyspora pogona

Examples

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Embodiment 1

[0037] A kind of technique of extracting butenyl spinosyn from Saccharopolyspora barbae fermented liquid, concrete steps are as follows:

[0038] (1) After fermentation, before putting into the tank, add sodium hydroxide solution with a concentration of 0.1-0.2g / mL to 100L fermentation broth, adjust the pH of the fermentation broth to 8.5, then raise the temperature, and add diatomaceous earth and perlite to filter in turn After adding diatomite and perlite, the concentration of diatomite and perlite is 0.015g / mL and 0.025g / mL respectively, mix evenly, heat up to 90°C, keep stirring for 1.5h, a large amount of flocculent suspensions are formed in the fermentation broth, and the pre- Treated fermented liquid; after pretreatment, the fermented liquid is filtered by a plate and frame filter press or a centrifuge, and the time required to complete solid-liquid separation is 1h. The content of butenyl spinosyn in the filtrate is 50 μg / mL, and the filter cake is collected; The colle...

Embodiment 2

[0048] A kind of technique of extracting butenyl spinosyn from Saccharopolyspora barbae fermented liquid, concrete steps are as follows:

[0049] (1) After fermentation, before putting into the tank, add sodium hydroxide solution with a concentration of 0.1-0.2g / mL to the 100L fermentation broth, adjust the pH of the fermentation broth to 9.5, then raise the temperature, and add diatomaceous earth and perlite to filter in turn After adding diatomite and perlite, the concentration of diatomite and perlite is 0.025g / mL and 0.035g / mL respectively, mix evenly, heat up to 95°C, keep stirring for 2.5h, a large amount of flocculent suspensions are formed in the fermentation broth, and the pre- Treated fermented liquid; after pretreatment, the fermented liquid is filtered by plate and frame filter press or centrifuge, the time required to complete solid-liquid separation is 40min, the content of butenyl spinosyn in the filtrate is 30 μg / mL, and the filter cake is collected; The collec...

Embodiment 3

[0057] A kind of technique of extracting butenyl spinosyn from Saccharopolyspora barbae fermented liquid, concrete steps are as follows:

[0058] (1) After fermentation, before putting into the tank, add sodium hydroxide solution with a concentration of 0.2-0.3g / mL to the 100L fermentation broth, adjust the pH of the fermentation broth to 9.0, then raise the temperature, and then add diatomaceous earth and perlite to aid filter After adding diatomite and perlite, the concentration of diatomite and perlite is 0.02g / mL and 0.04g / mL respectively, mix evenly, heat up to 95°C, keep stirring for 3 hours, a large amount of flocculent suspensions are formed in the fermentation broth, and pretreatment post-fermentation broth; after pretreatment, the fermentation broth was filtered with a plate-and-frame filter press or a centrifuge, and the time required to complete solid-liquid separation was 30 min. The content of butenyl spinosyn in the filtrate was 20 μg / mL, and the filter cake was ...

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Abstract

The invention discloses a process for extracting butenyl-spinosyn from fermentation liquor of Saccharopolyspora pogona. The process comprises steps of pretreatment of the fermentation liquor, soakingand extraction of dry fungus powder, concentration, extraction, crystallization and the like, and can obtain butenyl-spinosyn with a purity of more than 90%. In the crystallization process, dissolution crystallization and low-temperature crystallization are combined, so that content of butenyl-spinosyn A and butenyl-spinosyn D in the butenyl-spinosyn product can be controlled while recrystallization yield is guaranteed, and the content of the former is 5-8 times the content of the latter. The process has low cost, simple production equipment, high yield, and controllable product composition, and has industrial application prospects.

Description

technical field [0001] The invention relates to a method for extracting antibiotics from fermentation broth. More specifically, it relates to a process for extracting butenyl spinosyn from the fermentation broth of Saccharopolyspora. Background technique [0002] Butenyl-spinosyns (butenyl-spinosyns), also known as pogonins, are a class of macrocyclic endosyns with similar structures and the same insecticidal mechanism produced by the soil actinomycete Saccharopolyspora pogona. Esters insecticides. Butenyl spinosyn has the unique insecticidal mechanism of spinosad, and has strong feeding toxicity and rapid contact toxicity to pests. It mainly acts on nicotinic acetylcholine receptors (nicotinic acetylcholine receptor, nAchR) and γ-aminobutyric acid receptors (γ-amino-hutyric acid receptor, GABA), causing excitement by stimulating the nervous system, leading to non-functional muscle contraction , shivering, exhaustion and paralysis, etc., and eventually kill the pests. The...

Claims

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Application Information

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IPC IPC(8): C07H1/06C07H17/08
CPCC07H1/06C07H17/08
Inventor 王超郭超郭伟群常晓娇孙长坡
Owner ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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