Molecular markers linked to ppo inhibitor tolerance in soybeans

a technology of molecular markers and soybeans, applied in the field of molecular markers linked to soybean ppo inhibitor tolerance, can solve the problems of farmers' lower yields, and achieve the effect of negatively affecting the crop

Inactive Publication Date: 2014-08-21
PIONEER HI BRED INT INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0015]Soybean plants, seeds, tissue cultures, variants and mutants having tolerance or sensitivity to PPO inhibitor herbicides produced by the foregoing methods are also provided. Also provided herein are methods for controlling weeds in a crop by applying to the crop and any weeds affecting such crop an effective amount of such herbicide(s), either pre-emergent or post-emergent, such that the weeds are substantially controlled without substantially negatively impacting the crop.

Problems solved by technology

This increased tolerance decreases the effectiveness of glyphosate application and results in lower yields for farmers.

Method used

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  • Molecular markers linked to ppo inhibitor tolerance in soybeans
  • Molecular markers linked to ppo inhibitor tolerance in soybeans
  • Molecular markers linked to ppo inhibitor tolerance in soybeans

Examples

Experimental program
Comparison scheme
Effect test

example 1

Identification of Sulfentrazone Tolerant and Sensitive Soybean Lines-Herbicide Screening Bioassay and Intergroup Association Marker Based Diagnostic

[0264]Sulfentrazone is a PPO inhibitor and is the active ingredient in Authority® herbicide. Authority® 75DF (FMC Corp., Philadelphia, Pa., USA) is a 75% active ingredient formulation of sulfentrazone containing no other active ingredients.

Part 1: Herbicide Bioassay

[0265]One hundred sixteen (116) elite soybean lines were screened for sulfentrazone tolerance using the following protocol. Seed of soybean varieties with adequate seed quality, having greater than 85% warm germination were used.

Design and Replication:

[0266]After planting, entries were set up in a randomized complete block design, blocked by replication. Three replications per experiment were used. One or more of well established check variety were included in the experiment, such as available public sector check lines.

Non-tolerant check: Pioneer 9692, Asgrow A4715

Tolerant che...

example 2

Predication and Confirmation of Marker Based Selection for Response to PPO Chemistries in a Set of Diverse Public Soybean Lines

[0282]Marker haplotype data for a set of 17 diverse public soybean lines was determined for two QTL identified in Example 1 for Linkage Group L molecular markers P10649C-3 (approximate position 3.6) and S00224-1 (approximate position 12.0). The response of these lines to sulfentrazone herbicide was published by Hulting et al. (Soybean (Glycine max (L.) Merr.) cultivar tolerance to sulfentrazone. 2001 Science Direct, Vol. 20(8): 679-683). The phenotypic response was reported as a growth reduction index: plant height and visual injury as expressed as a percentage of check plot of each cultivar. Data for the marker haplotype on Linkage Group L and the herbicide bioassay results are presented in Table 3. Use of the molecular diagnostic P10649C-3 (linked QTL on Linkage Group L, approximate position 3.6) for this set of phentoyped soybean lines is 92% predictive o...

example 3

Predication and Confirmation of Marker Based Selection for Response to PPO Chemistries in a Set of Soybean Commercial Lines

[0284]Haplotype data for a set of 7 commercial soybean lines was determined for two QTL identified in the previous example for Linkage Group L molecular markers P10649C-3 (position 3.6) and S00224-1 (position 12.0). The response of these lines to sulfentrazone herbicide was determined by method used in Example 1. In addition, the same scale was used for scoring such that:[0285]9=Equivalent or better when compared to the tolerant check[0286]7=Very little damage or response noted.[0287]5=Intermediate response or damage[0288]3=Major damage, including stunting and foliar necrosis[0289]1=Severe damage, including severe stunting and necrosis; equivalent or worse when compared to the non-tolerant check

Data for the marker haplotype on Linkage Group L and the herbicide bioassay results are presented in Table 5. Use of either / both of these markers for this set of phentoyp...

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Abstract

This invention relates generally to the detection of genetic differences among soybeans. More particularly, the invention relates to soybean quantitative trait loci (QTL) for tolerance to protoporphyrinogen oxidase inhibitors, to soybean plants possessing these QTLs, which map to a novel chromosomal region, and to genetic markers that are indicative of phenotypes associated with protoporphyrinogen oxidase inhibitor tolerance. Methods and compositions for use of these markers in genotyping of soybean and selection are also disclosed.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation-in-part of application Ser. No. 12 / 506,498, filed Jul. 21, 2009, which claims priority under 35 U.S.C. §119(e) to provisional application Ser. No. 60 / 083,038 filed Jul. 23, 2008, herein incorporated by reference in their entirety.FIELD OF THE INVENTION[0002]This invention relates generally to the detection of genetic differences among soybeans.BACKGROUND OF THE INVENTION[0003]Soybeans (Glycine max L. Merr.) are a major cash crop and investment commodity in North America and elsewhere. Soybean oil is one of the most widely used edible oils, and soybeans are used worldwide both in animal feed and in human food production. Additionally, soybean utilization is expanding to industrial, manufacturing, and pharmaceutical applications. Weed management in soybean fields is important to maximizing yields. A recent development in soybean technology has been the development of herbicide-tolerant soybean varieties. G...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68A01H5/10
CPCC12Q1/6895A01H5/10C12Q2600/156C12Q2600/13C12Q2600/172A01H6/542A01H1/045A01N47/38C12N15/8274
Inventor CHAKY, JULIANFENGLER, KEVIN A.KLAIBER, JENNIFER A.KYLE, DONALDLI, BALINVOGT, MARK D.
Owner PIONEER HI BRED INT INC
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