Composition containing a gold (i) complex and cancer cells
a technology of complexes and cancer cells, applied in the field of therapeutic gold (i) complexes and pharmaceutical compositions thereof, can solve problems such as limiting the applicability of complexes
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Materials and Methods
[0140]Ethidium bromide (EB) sodium N,N-dimethyldithiocarbamate hydrate (DMDT), and sodium N,N-diethyldithiocarbamate hydrate (DEDT) were purchased from Sigma-Aldrich Tris-(hydroxymethyl)aminomethane (Tris buffer) (Merck), adenosine-5′-monophosphate disodium salt (5′-AMP), cytidine-5′-monophosphate disodium salt hydrate (5′-CMP), guanosine-5′-monophosphate disodium salt (5′-GMP), thymine-5′-monophosphate (5′-TMP), and CH3OH were obtained from Fluka Chemicals Co. and were stored at −20° C. Disodium salt of CT-DNA (calf thymus DNA) was purchased from Sigma Chem. Co. and was stored at 4° C. All reagents as well as solvents were used as received. Human breast cancer cell lines, MDA-MB231 and MCF7, were provided by American Type Culture Collection (ATCC) Cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum (FCS), penicillin (100 kU L−1) and streptomycin (0.1 g L−1) at 37° C. in a 5%, CO2-95% air atmosphere, MTT (3-(4,5-...
example 2
Synthesis of Therapeutic Gold(I) Complexes
[0141]Following the previous studies on mixed phosphine gold(I) dithiocarbamate compounds with antitumor activity and in continuation of the interest in the synthesis of gold(I) complexes to better understand the chemical and physical behavior of biologically relevant dithiocarmato(phosphine)gold(I) complexes, gold(I) complexes 1-5 were synthesized and fully characterized by elemental analysis, FT1R, NMR measurements and UV-Vis spectroscopic techniques (Altaf, M,: Monim-ul-Mehboob, M.; Seliman, A. A.; Sohail, M.; Wazeer, I. M.; Isab, A. A.:, Li, L.; Dhuna, V.; Bhatia, G.; Dhuna, K. Synthesis, Characterization and anticancer activity of gold(I) complexes that contain tri-teri-butylphosphine and dialkyldithiocarbamate ligands. Eur. J. Med. Chem. 2015, 95, 464-472; and Altaf M.; Monim-ul-Mehboob, M; Isab, A. A.; Bhatia, G.; Dhuna, K.; Altuwaijri, S. The synthesis, spectroscopic characterization and anticancer activity of new mono and binuclear ...
example 3
Electronic Spectra
[0144]Electronic spectra were obtained for the gold(I) complexes using Lambda 200, Perkin-Elmer UV-Vis spectrometer. UV-Vis spectroscopy was used to determine the stability of the complexes in DMSO. Electronic spectra were recorded on freshly prepared samples of each complex in a buffer at room temperature. The resulting UV-Vis absorption data are shown in Table 2.
TABLE 2λmax values derived from UV-Vis spectra for Au(I) complexes 1-5Complexλmax (nm)(Me)3PAu(S2CN{Me}2) (1)270, 321(Et)3PAu(S2CN{Me}2) (2)270, 320(Me)3PAu(S2CN{Et}2) (3)270, 321(Et)3PAu(S2CN{Et}2) (4)272, 333(i-Pr)3PAu(S2CN{Me}2) (5)270, 322
[0145]The optical electronic absorption spectra show a similar pattern for all of the complexes. The gold(I) completes 1-5 absorbed intensely at two regions, 270-272 nm and 321-333 nm, which were attributed to the intramolecular intraligand transition corresponding to π→π° in the NCS and CSS moieties, respectively (Yang, Y.; Zuo, B.; Li, J.; G. Chen, G. Studies on th...
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