Composition for preventing or treating pruritus
a technology for pruritus and composition, applied in the field of composition for preventing or treating pruritus, can solve the problems of insufficient knowledge of its characteristics, limited use of adrenocortical hormones or corticosteroids, and limited therapeutic effect, so as to prevent, improve or treat pruritus caused by various causes safely and effectively, without the risk of side effects
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[0085]Six-week-old, specific-pathogen-free male BALB / c mice were purchased from Orient Bio Korea Inc. (Gapyeong, Gyeonggi-do, Korea) and housed in a laminar flow cabinet. They were provided with standard solid chows ad libitum. At the onset of the test, the mice were 7-8 weeks old. All animal care and use were performed in accordance with the protocol of the Institutional Animal Care and Use Committee of the Chonbuk National University Medical School.
Preparation of Reagents
[0086]Isoflavone-based compounds genistein, daidzein, glycitein and equol were purchased from Sigma. They were dissolved in DMSO and diluted with distilled water. Care was taken such that the content of DMSO did not exceed 0.1%. Histamine, chloroquine and DNCB were also purchased from Sigma.
Induction of Pruritus
[0087]1. Histamine- and Chloroquine-Induced Pruritus
[0088]Pruritus was induced by subcutaneously injecting histamine and chloroquine to the mice. Specifically, 0.1 mL of 2 mg / mL histamine and 4 mg / mL chlo...
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