41results about How to "Fully closed" patented technology

The invention discloses a Taq DNA polymerase monoclonal antibody combination as well as a reaction system and application thereof. The invention firstly discloses the Taq DNA polymerase monoclonal antibody combination. The combination comprises a monoclonal antibody 4A8 and a monoclonal antibody 9B7. The invention further discloses application of the antibody combination in reducing non-specific amplification of Taq DNA polymerase and / or ensuring thermal stability of Taq DNA polymerase, and a reaction system and a kit containing the antibody combination. The antibody combination and the optimized reaction solution can fully seal the active region of Taq DNA polymerase under the condition of low temperature, effectively reduce non-specific amplification and ensure the thermal stability of the enzyme, and can still effectively maintain the durability of the enzyme activity under the condition of higher amplification cycle number.

The application discloses an overturning mechanism. The overturning mechanism is used for rotating connection of a first part and a second part which can be opened and closed by overturning on electronic equipment. The overturning mechanism comprises a rotary shaft and a self-locking part, wherein the rotary shaft is rotatably connected with the first part and the second part; the self-locking part is sheathed on the rotary shaft and is in frictional contact with the rotary shaft; and with the rotation of the rotary shaft, the rotational friction generated between the self-locking part and the rotary shaft is increased gradually. The overturning mechanism disclosed by the invention has the advantages that compared with the existing mode for obtaining the friction by a plane vertical to the axis of the rotary shaft, since the self-locking part is sheathed on the rotary shaft and is in frictional contact with the rotary shaft, the rotational friction generated by the self-locking part and the rotary shaft is distributed along the circumferential direction of the rotary shaft, so that even though the size of the plane of the self-locking part is reduced, the size of the friction between the rotary shaft and the self-locking part sheathed thereon is also influenced, the working performance of the electronic equipment also can not be reduced when the whole size of the electronic equipment is reduced, even the working performance of the electronic equipment can be further improved. The invention also provides the electronic equipment with the overturning mechanism.

The invention provides a method of separating and recovering aluminum, potassium, and ammonium from a mixed solution containing aluminum sulfate, potassium sulfate, and ammonium sulfate. The method comprises following steps: evaporating and condensing the mixed solution, cooling the condensate to carry out crystallization to obtain mixed crystals of potassium alum and ammonium alum, burning the mixed crystals at a high temperature to prepare alumina, and recovering potassium sulfate and ammonium sulfate at the same time. The provided method can efficiently recover aluminum, potassium, and ammonium, and obtains high purity alumina, potassium sulfate, and ammonium sulfate, furthermore, the energy consumption is low, the investment is little, the production is carried out in an enclosed environment, no waste solid, waste gas or wastewater is discharged, the devices are easy to enlarge, and the industrial application prospect is good.

The invention relates to the field of medicine, and particularly relates to a Leber's hereditary optic neuropathy (LHON) gene diagnosis kit or reagent comprising a PCR primer mixture liquid; the primer mixture liquid comprises a pentose nucleic acid (PNA) probe for sealing a wild-type mitochondrion (which is a normal human mitochondrion without generation of mutation) deoxyribonucleic acid (DNA) and forward and reverse primers for amplifying mutant-type mitochondrion DNA. The kit or reagent has the advantages of simple operation, high sensitivity and good specificity, and is an efficient, sensitive, stable and specific LHON gene diagnosis detection technology.

The invention discloses a three-station isolation switch which comprises a three-station isolation switch body and an operating mechanism, and is characterized in that the three-station isolation switch body comprises a moving contact assembly, a static contact assembly, a grounding contact assembly, a moving contact and a moving contact driving mechanism, and the moving contact is hinged with themoving contact assembly; the moving contact driving mechanism is connected with the moving contact and used for driving the moving contact to rotate around the hinge point of the moving contact, thestatic contact assembly and the grounding contact assembly are both located on the motion trail of the moving contact, and the operating mechanism is connected with the moving contact driving mechanism and used for controlling the driving action of the moving contact driving mechanism. The isolation switch is provided with a stable main body framework, the position design of each contact assemblyis reasonable, the stable moving contact driving mechanism drives the moving contact to move to each contact assembly to finish the turn-on action, the turn-on in place is ensured, and stable protection is provided for a power supply system.

The invention discloses rigid combination rod shutter blade mechanisms capable of achieving twisted deformation and an operating system. Each blade mechanism comprises a pair of fixing plates and a plurality of blade rods with variable length arranged side by side between the pair of fixing plates. Each blade rod is movably connected with the fixing plates. The side of each of the pair of fixing plates is provided with an end plate provided with a rotating shaft. The operating system comprises a shutter frame. The plurality of blade mechanisms are disposed between a left side frame and a rightside frame of the shutter frame from top to bottom adjacently, wherein the left side frame and the right side frame are respectively provided with a driving mechanism for driving rotating shafts on the blade mechanisms to rotate, and the driving mechanisms on the left side frame and the right side frame are adjusted synchronously or separately. The overall opening / closing of all the blade mechanisms in the window frame can be achieved, or a part of the rigid combination rod blades can be gradually closed / gradually opened in the length direction.

The invention discloses a parallel light beam apparatus for measuring the biometric dosage of ultraviolet rays, relating to the technical field of measurement instruments. A new product of light beam apparatus is provided by key technology of a biologic assay carried out by radiation of vertically downward parallel ultraviolet light beams and integrated measurement of low-pressure ultraviolet rays and intermediate-pressure ultraviolet rays, wherein a workbench is fixed to a lifter and an electric cabinet, the upper end of the lifter is fixed to a supporting table, the upper end of the electric cabinet is fixed to a box body, the left end of the box body is fixed to a lamp holder, a base plate in the box body is fixed to a cooling sleeve and a support, the cooling sleeve is fixed to a muffle, the base plate of the box body is fixed to a shutter casing, a base plate in the shutter casing is fixed to an air cylinder, the base plate of the shutter casing is fixed to a telescopic cylinder, the end head of an air cylinder bar is rotationally and movably connected with an eccentric wheel, the eccentric wheel is rotationally and movably connected with a light barrier, an electromagnetic stirrer is placed on the supporting table, a measuring cup is placed on the electromagnetic stirrer, the electric cabinet is electrically connected with the lamp holder, the air cylinder and the electromagnetic stirrer, the lamp holder is electrically connected with a lamp tube, and the lamp tube is supported and installed on the support. The parallel light beam apparatus is applied to biologic assay, fully considers practical factors of processed water, irradiates by efficient dosage, reaches the purpose of complete sterilization and is suitable for intermediate and low pressure ultraviolet lamps.

The invention provides a wind guide mechanism and an air-conditioner. The wind guide mechanism comprises a wind guide plate, a power assembly and a guide assembly, wherein the wind guide plate is arranged at a wind outlet of electric appliance, the power assembly drives the wind guide plate to move to open or close the wind outlet, the wind guide assembly is used for guiding the wind guide plate to move, when the wind outlet is closed, the wind guide plate closes the wind outlet completely, and when the wind outlet is opened, the wind guide plate separates the wind outlet into multiple wind output areas. According to the wind guide mechanism, wind can be output from the upper side and the lower side at the same time, thus condensing of the wind guide plate is avoided, the wind guide platecan achieve complete closing, and has attractive appearance, the power assembly and the guide assembly are designed elaborately, the requirement of the wind guide plate for torque of a motor is lower,the angle speed and the horizontal moving speed of the wind guide plate are large, the wind outlet can be opened or closed more rapidly and saves more energy, a single plate is adopted as the wind guide plate and matched with the guide assembly, the single motor and a single connecting rod can be used for driving the wind guide plate, and the cost is lower.

The invention discloses a tree seed collecting machine with adjustable shear plane and a using method of the tree seed collecting machine. The tree seed collecting machine with the adjustable shear plane comprises a cutting mechanism, a lever pulling mechanism, a rod body and a film funnel collecting machine; the cutting mechanism mainly comprises a rod head, a movable block, a first-level pullingrope, a second-level pulling rope, a main blade, a deputy blade, a pin roll II, and a direction guiding sliding wheel; the lever pulling mechanism mainly comprises a holding handle, an L-shaped trigger, and a supporting arm; the supporting arm is fixedly installed on one side of the holding handle, and a pin roll I is arranged at the front end of the supporting arm. According to the tree seed collecting machine with the adjustable shear plane and the using method of the tree seed collecting machine, a difficult problem that a special tree seed collecting tool lacks in the forestry seedling production is solved, a simple, light, convenient, safe, efficient and practical forest seed collecting machine is provided for the forestry tree seed collecting production, the labor intensity of workers is reduced, safety and the working efficiency are improved, and the forestry seedling production is better serviced.

The invention discloses a sewage treatment reaction tank and a sewage treatment method. The sewage treatment reaction tank comprises a reaction tank body, a stirring unit, a power unit, a precipitatedischarge unit, a sludge dewatering agent storage unit, a disinfectant adding unit and a sludge separation unit, wherein a tank cover is arranged at the top of the reaction tank body, the interior ofthe reaction tank body is divided into a precipitation cavity, an anaerobic cavity and a disinfection cavity from left to right through a first partition plate and a second partition plate, at least three branch pipes are arranged on the left side of the reaction tank body in a penetrating mode and connected with the side face of a water conveying pipe, and a first water valve is arranged at the end of the water conveying pipe. According to the invention, only one power mechanism needs to be adopted for stirring and injecting a sludge dewatering agent, so that electric power is saved, injecting and stirring can be conducted at the same time, precipitated sludge is convenient to collect and take out, subsequent sludge precipitation is not affected, the use efficiency is high, anaerobic treatment can be rapidly achieved, a disinfectant can be added subsequently, and the sewage treatment effect is good.

The invention discloses a high-temperature-resistant hole sealing agent for a hot-dip plating sink roll as well as a preparation method and a coating method, and belongs to the field of sink roll surface treatment. The hole sealing agent is mainly prepared from the following components in parts by weight: 100 parts of silica sol, 60 to 100 parts of alumina sol, 100 to 240 parts of siloxane, 104 to 264 parts of hexagonal boron nitride dispersion liquid, 1.82 to 7.04 parts of cerous nitrate and a trace amount of hydrochloric acid solution. The obtained high-temperature-resistant hole sealing coating is an organic-inorganic hole sealing system, not only has high wear resistance and heat resistance of an inorganic coating, but also has corrosion resistance of an organic coating, and meanwhile, the adhesive force of the hole sealing coating is improved.

The invention relates to a sweet potato peeling device comprising a peeling device and a discharge device, wherein the peeling device comprises a peeling box, a plurality of brush rolls and driving rolls with smooth surfaces are arranged in the peeling box, the brush rolls and the driving rolls are alternatively arranged along the internal arc structure of the peeling box, one ends of the brush rolls and the driving rolls are respectively provided with mutually meshed gears, a pressure spray pipe is horizontally arranged above the peeling box, nozzles corresponding to the interior of the peeling box are uniformly distributed on the pressure spay pipe, and a discharge hole is formed in the side wall of the peeling box; the discharge device comprises a material conveying groove and a rotary baffle used for closing the discharge hole, the material conveying groove is correspondingly arranged below the discharge hole, a rotary shaft is fixedly connected to the rotary baffle, the rotary shaft is hinged to the part, above the discharge hole, of the peeling box, a handle is connected to one end of the rotary shaft, and a locking slot used for clamping the handle is formed in the peeling box. By implementing the technical scheme, the problems of an existing sweet potato peeling device that the operation procedure is complicated, the labor load is large or equipment is blocked are solved.

The invention discloses a closing device for a preformed hole in an artificial blood vessel and a conveying device of the closing device. The closing device comprises an outer side closing film, an inner side closing film and a positioning ring, wherein the positioning ring is of a spiral structure and comprises two end parts and a middle, the middle is connected between the two end parts, and thetwo end parts of the positioning ring are respectively connected to the outer side closing film and the inner side closing film. When the closing device is used for closing the preformed hole in theartificial blood vessel, the positioning ring penetrates through the preformed hole, and the outer side closing film and the inner side closing film are respectively located on the outer and inner sides of the pipe wall of the artificial blood vessel and correspond to the to-be-closed preformed hole. By utilizing the design that the outer side closing film and the inner side closing film are respectively located on the outer and inner sides of the pipe wall of the artificial blood vessel, the outer side closing film is selectively put into a false lumen on the outer side of an aortal artificial blood vessel, the inner side closing film is put into a true lumen on the inner side of the aortal artificial blood vessel, and the preformed hole and a breaking hole are closed together, so that the risk that openings of adjacent branched arteries are closed by the true lumen is avoided, meanwhile, the breaking hole is relatively adequately closed, and the validity and the safe reliability areimproved.

The invention provides an extracellular vesicle continuous separation and purification device which comprises an extracellular vesicle separation and purification device and a main control module which are connected with each other, the extracellular vesicle separation and purification device comprises an ultrafilter module for separating extracellular vesicles with different particle size ranges, a pressurizing liquid inlet module for providing positive pressure ultrafiltration power and fluid flowing positive pressure pushing power in the ultrafilter module, and a concentration end negative pressure aspirator module for providing fluid flowing negative pressure suction power in the ultrafilter module, the filter end negative pressure aspirator module is used for providing negative pressure ultrafiltration power in the ultrafilter module; and the pipeline temperature control module is used for maintaining the temperature of liquid to be separated in a pipeline and maintaining the quality of extracellular vesicles. The device disclosed by the invention can continuously maintain the filtering efficiency of the ultrafiltration membrane, and realizes the purposes of totally-closed continuous flow high-flux difference aperture series dynamic ultrafiltration separation and purification of extracellular vesicles under the conditions of positive pressure of a liquid inlet and negative pressure of a concentration end and a filtering end.

The invention discloses a production method of a stable geothermal floor. The production method includes drying and processing a floor blank into a floor; subjecting the floor to surface glue injection and drying three times; performing sanding and painting on the floor to obtain the finished floor. The method has the advantages that production efficiency is improved, floor stability is promoted, and the stable geothermal floor is less prone to deformation and cracking.

The invention relates to the technical field of oil exploitation, and discloses a blowout preventer for sealing wellheads, comprising a blowout preventer base, two ends of the blowout preventer base are provided with symmetrical hydraulic cylinders, and one end of the hydraulic cylinder It communicates with No. 1 channel, and the other end of the hydraulic cylinder communicates with No. 2 channel. A hydraulic rod is movably socketed in the hydraulic cylinder, and a central pipeline through which is opened in the center of the blowout preventer base. The present invention uses the designed positioning base, ram, and self-locking ram so that after the ram is closed, it can be lifted up under the pressure impact of the blowout material, so that both sides of the top of the ram are close to the self-locking ram, and the Under the pressure impact of the blowout material, when the positioning base has a force to open to both sides, the ram can transmit the force to the BOP base through the self-locking ram, so that the ram forms a self-locking and prevents the ram from being separated by force situation, and make the blowout preventer get rid of the locking methods of manual locking and electric locking.

The invention discloses a Taq DNA polymerase monoclonal antibody combination, a polymerase reaction system containing the same and application of the Taq DNA polymerase monoclonal antibody combination. The invention firstly discloses the Taq DNA polymerase monoclonal antibody combination, and the combination comprises a monoclonal antibody 4A8 and a monoclonal antibody 5H6. The invention further discloses application of the antibody combination in reducing non-specific amplification of Taq DNA polymerase and / or ensuring the thermal stability of the Taq DNA polymerase, the reaction system containing the combination, and a kit. The antibody combination and an optimized reaction solution can fully seal an active region of the Taq DNA polymerase under the condition of low temperature, effectively reduce non-specific amplification and ensure the thermal stability of the enzyme, and can still effectively maintain the durability of the enzyme activity under the condition of higher cycle number of amplification.

The invention belongs to the technical field of film forming agents and discloses a self-cleaning phosphorus-free film forming agent and a preparation method and application thereof to metal plates. The self-cleaning phosphorus-free film forming agent is prepared from, by weight, 1-6% of fluorozirconate, 1-6% of epoxy silane, 0.5-3% of a corrosion-resistant auxiliary agent, 0.5-2% of an auxiliaryactivator and the balance deionized water, wherein the corrosion-resistant auxiliary agent refers to any one or two of sodium molybdate and ammonium molybdate, and the auxiliary activator refers to any one or more of stearic acid, sodium alkyl benzene sulfonate, polyalcohol and polyoxyethylene polyoxypropylene ether. The self-cleaning phosphorus-free film forming agent is obtained by modificationthrough epoxy silane and the auxiliary activator and used for pretreatment of the metal plates, and uniformity in film forming, film layer compactness, freeness of rust, great salt spray resistance and long service life are realized; a degreasing station is avoided, multiple washing stations are reduced, and accordingly space saving and cost reduction are realized, sewage discharge is reduced, andconvenience in coating is achieved.

In an intake manifold that has charge-motion-control valves (CMCVs), a gap exists between the flapper valves and the wall of the intake runners. Although the gap is maintained as small as practical, it cannot be eliminated because manufacturing tolerances and temperature variations to which the intake manifold is subjected must be accommodated to prevent binding of the flapper valves. Some flow makes an end run through the gap leading to undesirable fluid mechanics. Disclosed herein is a seal that is positioned to rest gently upon the flapper valve near the gap such that the gap is substantially sealed off while applying a modest force on the CMCV so that the actuation torque is minimally impacted. The seal has a press-in-place portion inserted into a pocket formed in the manifold to hold it in place and a lip portion that extends out from the press-in-place portion to obstruct the gap.

The invention discloses a non-contact rotary electric energy transmitting device, which includes a shell, a spindle, a stator, a rotor, input power and output circuit, the stator and the rotor are arranged with triphase windings, the character lies in: there have two sets of stator and rotor which are assembled in the shell coaxially, the spindle is hollow, wire apertures are arranged on the shell and the side walls of the spindle. The triphase output wire of the input and output circuit are leaded out from the aperture; the two stators are supplied with alternate current with the reversal phase, the output of the two rotors are overlapped in series after passing the commutating circuit. The merits are: it is coaxial double stator and double rotor structure, the series overlapping of output potential and conducting with the reverse phase alternate current, the torque generated by the two rotors is equal, the direction is reverse, which two can be counteracted, it will not rotate automatically, it can rotate angle with outer driving mechanism, and the voltage fluctuation, thus, it realizes to transmit the electric energy on stator side to rotary rotor side in non-contact way.

The invention discloses an automatic switching-on and switching-off device of an intelligent miniature circuit breaker. A worm is installed on a rotating shaft of a driving motor, the worm is used incooperation with a meshing gear set, a half gear in a fourth gear is used in cooperation with a handle driving gear, and the handle driving gear is installed on a handle driving square shaft; a straight gear of the first gear is mounted above a helical gear; a tripping shifting block is installed on the fourth gear, a tripping rod is installed on a side surface of the fourth gear, the tripping rodis used in cooperation with the tripping shifting block, and the tripping rod is fixedly installed on a body linkage shaft; the handle driving gear is provided with a switching-on auxiliary elastomerwhich is used in cooperation with the half gear of the fourth gear; and the body linkage shaft and the handle driving square shaft are connected with the circuit breaker. The device is reasonable instructural design, reliable in action in use and very high in practicability; and switching-on reliability of the product can be improved, a service life of the product is prolonged, and accidents arereduced.

The invention provides a method of separating and recovering aluminum, potassium, and ammonium from a mixed solution containing aluminum sulfate, potassium sulfate, and ammonium sulfate. The method comprises following steps: evaporating and condensing the mixed solution, cooling the condensate to carry out crystallization to obtain mixed crystals of potassium alum and ammonium alum, burning the mixed crystals at a high temperature to prepare alumina, and recovering potassium sulfate and ammonium sulfate at the same time. The provided method can efficiently recover aluminum, potassium, and ammonium, and obtains high purity alumina, potassium sulfate, and ammonium sulfate, furthermore, the energy consumption is low, the investment is little, the production is carried out in an enclosed environment, no waste solid, waste gas or wastewater is discharged, the devices are easy to enlarge, and the industrial application prospect is good.

A device for gaining solar energy comprises a integral unit with a casing (10..13). The casing comprises reflection means (20) with a hollow reflector surface (25) and is closed off by refraction means (30) with an entry surface (35) for entering sunlight. The refraction means and reflection means are each rotatably arranged around a rotation axis (71, 72) and their orientation relative to each other is continuously adjusted to the current position of the sun in the sky using positioning means provided for that purpose. The casing comprises a number of dustproof, but nevertheless ventilating chambers. The first chamber (100) is situated between the refraction means (30) and the reflection means (20) and comprises energy-conversion means (40) at least near to the focal point of the reflector surface (25). A second chamber (200) is situated between the reflection means (20) and the base part (13) of the casing and comprises positioning means (51, 53) for rotating the refraction means (30) and the reflection means (20).

The embodiment of the invention provides an electrosurgical generator, an electrosurgical system and a control method of the electrosurgical system. The method comprises the steps that after it is determined that two electrodes of a tool effectively clamp tissue, multiple sub-processes are executed in sequence, and each sub-process comprises a state judgment stage and a tissue fusion stage; wherein at least one control parameter and at least one end parameter of the current sub-process are determined according to at least one impedance parameter and at least one time parameter of the tissue in the state judgment stage of each sub-process; in the tissue fusion stage of each sub-process, energy is output to the tissue according to at least one control parameter of the current sub-process, and whether the current sub-process is ended or not is determined according to at least one ending parameter of the current sub-process. Therefore, according to the technical scheme provided by the embodiment of the invention, the tissue closing process can be adaptively controlled, the energy output to the tissue is dynamically and accurately controlled according to the closing state of the tissue, and the success rate of tissue closing and the bursting pressure of the tissue are improved.