41results about How to "Fully closed" patented technology

The invention relates to the field of medicine, and particularly relates to a Leber's hereditary optic neuropathy (LHON) gene diagnosis kit or reagent comprising a PCR primer mixture liquid; the primer mixture liquid comprises a pentose nucleic acid (PNA) probe for sealing a wild-type mitochondrion (which is a normal human mitochondrion without generation of mutation) deoxyribonucleic acid (DNA) and forward and reverse primers for amplifying mutant-type mitochondrion DNA. The kit or reagent has the advantages of simple operation, high sensitivity and good specificity, and is an efficient, sensitive, stable and specific LHON gene diagnosis detection technology.

The invention discloses a parallel light beam apparatus for measuring the biometric dosage of ultraviolet rays, relating to the technical field of measurement instruments. A new product of light beam apparatus is provided by key technology of a biologic assay carried out by radiation of vertically downward parallel ultraviolet light beams and integrated measurement of low-pressure ultraviolet rays and intermediate-pressure ultraviolet rays, wherein a workbench is fixed to a lifter and an electric cabinet, the upper end of the lifter is fixed to a supporting table, the upper end of the electric cabinet is fixed to a box body, the left end of the box body is fixed to a lamp holder, a base plate in the box body is fixed to a cooling sleeve and a support, the cooling sleeve is fixed to a muffle, the base plate of the box body is fixed to a shutter casing, a base plate in the shutter casing is fixed to an air cylinder, the base plate of the shutter casing is fixed to a telescopic cylinder, the end head of an air cylinder bar is rotationally and movably connected with an eccentric wheel, the eccentric wheel is rotationally and movably connected with a light barrier, an electromagnetic stirrer is placed on the supporting table, a measuring cup is placed on the electromagnetic stirrer, the electric cabinet is electrically connected with the lamp holder, the air cylinder and the electromagnetic stirrer, the lamp holder is electrically connected with a lamp tube, and the lamp tube is supported and installed on the support. The parallel light beam apparatus is applied to biologic assay, fully considers practical factors of processed water, irradiates by efficient dosage, reaches the purpose of complete sterilization and is suitable for intermediate and low pressure ultraviolet lamps.

The invention discloses a tree seed collecting machine with adjustable shear plane and a using method of the tree seed collecting machine. The tree seed collecting machine with the adjustable shear plane comprises a cutting mechanism, a lever pulling mechanism, a rod body and a film funnel collecting machine; the cutting mechanism mainly comprises a rod head, a movable block, a first-level pullingrope, a second-level pulling rope, a main blade, a deputy blade, a pin roll II, and a direction guiding sliding wheel; the lever pulling mechanism mainly comprises a holding handle, an L-shaped trigger, and a supporting arm; the supporting arm is fixedly installed on one side of the holding handle, and a pin roll I is arranged at the front end of the supporting arm. According to the tree seed collecting machine with the adjustable shear plane and the using method of the tree seed collecting machine, a difficult problem that a special tree seed collecting tool lacks in the forestry seedling production is solved, a simple, light, convenient, safe, efficient and practical forest seed collecting machine is provided for the forestry tree seed collecting production, the labor intensity of workers is reduced, safety and the working efficiency are improved, and the forestry seedling production is better serviced.

The invention provides an extracellular vesicle continuous separation and purification device which comprises an extracellular vesicle separation and purification device and a main control module which are connected with each other, the extracellular vesicle separation and purification device comprises an ultrafilter module for separating extracellular vesicles with different particle size ranges, a pressurizing liquid inlet module for providing positive pressure ultrafiltration power and fluid flowing positive pressure pushing power in the ultrafilter module, and a concentration end negative pressure aspirator module for providing fluid flowing negative pressure suction power in the ultrafilter module, the filter end negative pressure aspirator module is used for providing negative pressure ultrafiltration power in the ultrafilter module; and the pipeline temperature control module is used for maintaining the temperature of liquid to be separated in a pipeline and maintaining the quality of extracellular vesicles. The device disclosed by the invention can continuously maintain the filtering efficiency of the ultrafiltration membrane, and realizes the purposes of totally-closed continuous flow high-flux difference aperture series dynamic ultrafiltration separation and purification of extracellular vesicles under the conditions of positive pressure of a liquid inlet and negative pressure of a concentration end and a filtering end.

The invention relates to a preformed sound-insulation rubber block and an installation method for the preformed sound-insulation rubber block, which are applied in a cavity of an automobile. The preformed sound-insulation rubber block comprises a support body, one or a plurality of installation buckles, and a cone-shaped through hole of a water manifold, wherein the support body is in a shape corresponding to the cross section of the cavity of the automobile; one or a plurality of installation buckles is / are arranged on the support body and is / are used for fixing the sound-insulation rubber block on a metal plate of the finished automobile; and the cone-shaped through hole of the water manifold is arranged in the middle of the support body and is used for penetrating through the water manifold.

The invention relates to the technical field of oil exploitation, and discloses a blowout preventer for sealing wellheads, comprising a blowout preventer base, two ends of the blowout preventer base are provided with symmetrical hydraulic cylinders, and one end of the hydraulic cylinder It communicates with No. 1 channel, and the other end of the hydraulic cylinder communicates with No. 2 channel. A hydraulic rod is movably socketed in the hydraulic cylinder, and a central pipeline through which is opened in the center of the blowout preventer base. The present invention uses the designed positioning base, ram, and self-locking ram so that after the ram is closed, it can be lifted up under the pressure impact of the blowout material, so that both sides of the top of the ram are close to the self-locking ram, and the Under the pressure impact of the blowout material, when the positioning base has a force to open to both sides, the ram can transmit the force to the BOP base through the self-locking ram, so that the ram forms a self-locking and prevents the ram from being separated by force situation, and make the blowout preventer get rid of the locking methods of manual locking and electric locking.

The invention discloses a Taq DNA polymerase monoclonal antibody combination, a polymerase reaction system containing the same and application of the Taq DNA polymerase monoclonal antibody combination. The invention firstly discloses the Taq DNA polymerase monoclonal antibody combination, and the combination comprises a monoclonal antibody 4A8 and a monoclonal antibody 5H6. The invention further discloses application of the antibody combination in reducing non-specific amplification of Taq DNA polymerase and / or ensuring the thermal stability of the Taq DNA polymerase, the reaction system containing the combination, and a kit. The antibody combination and an optimized reaction solution can fully seal an active region of the Taq DNA polymerase under the condition of low temperature, effectively reduce non-specific amplification and ensure the thermal stability of the enzyme, and can still effectively maintain the durability of the enzyme activity under the condition of higher cycle number of amplification.

The invention belongs to the technical field of film forming agents and discloses a self-cleaning phosphorus-free film forming agent and a preparation method and application thereof to metal plates. The self-cleaning phosphorus-free film forming agent is prepared from, by weight, 1-6% of fluorozirconate, 1-6% of epoxy silane, 0.5-3% of a corrosion-resistant auxiliary agent, 0.5-2% of an auxiliaryactivator and the balance deionized water, wherein the corrosion-resistant auxiliary agent refers to any one or two of sodium molybdate and ammonium molybdate, and the auxiliary activator refers to any one or more of stearic acid, sodium alkyl benzene sulfonate, polyalcohol and polyoxyethylene polyoxypropylene ether. The self-cleaning phosphorus-free film forming agent is obtained by modificationthrough epoxy silane and the auxiliary activator and used for pretreatment of the metal plates, and uniformity in film forming, film layer compactness, freeness of rust, great salt spray resistance and long service life are realized; a degreasing station is avoided, multiple washing stations are reduced, and accordingly space saving and cost reduction are realized, sewage discharge is reduced, andconvenience in coating is achieved.

The invention discloses a non-contact rotary electric energy transmitting device, which includes a shell, a spindle, a stator, a rotor, input power and output circuit, the stator and the rotor are arranged with triphase windings, the character lies in: there have two sets of stator and rotor which are assembled in the shell coaxially, the spindle is hollow, wire apertures are arranged on the shell and the side walls of the spindle. The triphase output wire of the input and output circuit are leaded out from the aperture; the two stators are supplied with alternate current with the reversal phase, the output of the two rotors are overlapped in series after passing the commutating circuit. The merits are: it is coaxial double stator and double rotor structure, the series overlapping of output potential and conducting with the reverse phase alternate current, the torque generated by the two rotors is equal, the direction is reverse, which two can be counteracted, it will not rotate automatically, it can rotate angle with outer driving mechanism, and the voltage fluctuation, thus, it realizes to transmit the electric energy on stator side to rotary rotor side in non-contact way.

The invention discloses a reverse self-locking mechanism of a trigger switch. The mechanism comprises a chassis, a housing, and a trigger which is mounted between the chassis and the housing, wherein a reverse self-locking device is movably mounted on the housing; a reverse self-locking button for controlling the reverse self-locking device is mounted at the outer side of the housing; a reverse self-locking returning device which is connected to the trigger is mounted between the reverse self-locking button and the housing; another reverse self-locking returning device is mounted between the trigger and the chassis; the reverse self-locking device comprises a limiting groove and a locking plate which are arranged in the chassis; the limiting groove is formed in the chassis; the locking plate is movably mounted into the limiting groove in the chassis. The reverse self-locking mechanism of the trigger switch is simple in structure; a reverse self-locking switch chassis and the locking plate are re-designed, namely, the locking plate is moved into the switch chassis, so that the reverse self-locking reliability can be achieved, and the switch chassis can be completely closed, and as result, the dust preventing performance can be effectively improved, and the personal safety of a user can be protected.

In an intake manifold with a charge motion control valve (CMCV), there is a gap between the flapper valve and the wall of the intake runner. Although the gap is kept as small as possible, it cannot be eliminated due to the manufacturing tolerances and temperature variations that the intake manifold is subjected to to prevent binding of the flapper valves. Some airflow detours through the gap, resulting in undesirable hydrodynamics. Disclosed herein is a seal positioned to rest lightly on the flapper valve close to the gap such that the gap is substantially sealed when moderate force is applied on the CMCV such that actuation torque is minimally affected. The seal has a press-in-place portion inserted into a pocket formed in the manifold to hold it in place, and a lip portion extending from the press-in-place portion to block the gap.

The invention provides a method of separating and recovering aluminum, potassium, and ammonium from a mixed solution containing aluminum sulfate, potassium sulfate, and ammonium sulfate. The method comprises following steps: evaporating and condensing the mixed solution, cooling the condensate to carry out crystallization to obtain mixed crystals of potassium alum and ammonium alum, burning the mixed crystals at a high temperature to prepare alumina, and recovering potassium sulfate and ammonium sulfate at the same time. The provided method can efficiently recover aluminum, potassium, and ammonium, and obtains high purity alumina, potassium sulfate, and ammonium sulfate, furthermore, the energy consumption is low, the investment is little, the production is carried out in an enclosed environment, no waste solid, waste gas or wastewater is discharged, the devices are easy to enlarge, and the industrial application prospect is good.