The utility model relates to the technical field of detection kits, and discloses a detection kit for detecting the content of lobetyolin in traditional Chinese medicinal materials. The upper surface of the outer frame supporting frame is connected with a detachable elisa plate coated with lobetyolin
antigen or a detachable elisa plate coated with goat-anti-mouse secondary
antibody, one side of the outer frame supporting frame is provided with a placing frame, and the side edge of the placing frame is connected with the inner side of the detection
reagent inner box; an
enzyme marker placing tube, an anti-lobetyolin
monoclonal antibody diluent placing tube, a developing solution placing tube, a stop solution placing tube, a sample
diluent placing tube, six lobetyolin
standard solution placing tubes with different concentrations and washing concentrated solution placing tubes are arranged on the placing rack. According to the detection kit for detecting the content of lobetyolin in the traditional Chinese medicinal materials, manual shaking is reduced, meanwhile, a plurality of sample test tubes can be placed in the
test tube placing box at the same time, and the shaking efficiency is improved.