Radix bupleuri tissue culturing fast seedling-cultivating method

A technology of tissue culture and Bupleuri, applied in horticultural methods, botany equipment and methods, cultivation, etc., can solve the problems of different species, medium composition, culture conditions and transplanting management conditions, and different varieties, and achieve cost Low, shorten the seedling cycle, easy to renew the effect

Inactive Publication Date: 2009-12-09
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the types of plants are different, the varieties are different, and even the explants selected for the same variety are different. There are also great differences in the medium components, culture conditions and transplanting management conditions required for tissue culture.

Method used

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  • Radix bupleuri tissue culturing fast seedling-cultivating method
  • Radix bupleuri tissue culturing fast seedling-cultivating method
  • Radix bupleuri tissue culturing fast seedling-cultivating method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The young stems of Bupleurum bupleuri axillary buds taken from the field of Lingchuan, Shanxi were used as explants, and the floating soil was washed away by running water, and cut into 3cm-5cm long sections. Soaked in 75% ethanol for 30s, 0.1% HgCl 2 Disinfect with aqueous solution for 10 minutes, rinse with sterile water for 4 times, cut into 20 single-bud stem segments of about 0.5 cm, and inoculate them in a medium containing 6-BA 1.0 mg / L, KT 0.2 mg / L, sucrose 3.0%, pH 5.8 B 5 Cultured on the culture medium at 25±2°C, 2000lux, and 14h / d light to induce bud induction and rapid proliferation, and get clustered buds after 3 weeks (such as figure 1 ). After 4 weeks of subculture, a large number of rootless seedlings were obtained after 3 subcultures, which were transferred to B containing 3.0% sucrose and pH 5.8. 5 Basic medium for strong seedlings.

[0030]Cultivate for about 15 days. When the rootless seedlings grow to a height of about 2 cm, select the vigorous ...

Embodiment 2

[0034] Take the stem section of Bupleurum bupleurum with axillary buds from the field of Taigu, Shanxi (originating in Zuoquan, Shanxi), wash away the floating soil with running water, and cut into 3cm-5cm long sections. Soaked in 75% ethanol for 30s, 0.1% HgCl 2 Disinfect with aqueous solution for 10 minutes, rinse with sterile water for 4 times, cut into single-bud stem segments of about 0.5 cm, and inoculate them with B supplemented with 6-BA 0.5 mg / L, KT 0.1 mg / L, and NAA 0.1 mg / L. 5 In the culture medium, cultivate and induce the germination of axillary buds at 25±2°C, 2000lux, and 14h / d light. After 2 weeks, the axillary buds germinate and form rootless seedlings. After 4 weeks, the buds multiply by about 3 times.

[0035] The resulting rootless seedlings are further cut into single-bud stem segments, and transferred to the bud proliferation medium obtained by screening. The composition of the culture medium is: B 5 +6-BA 1.0mg / L+KT 0.2mg / L+3.0% sucrose, pH 5.8. Durin...

Embodiment 3

[0039] The stem section of Bupleurum angustifolia taken from the field of Taigu, Shanxi was sterilized and inoculated into a bud induction and proliferation medium, and the culture conditions and medium were the same as in Example 1 to obtain in vitro plantlets of Bupleurum angustifolia.

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Abstract

The invention provides a tissue culture rapid propagation method for medicinal bupleurum. The method chooses a stem with bupleurum bud as an explant and inoculates the explant in a B5 culture medium with additional 6-BA 1.0mg / L and KT 0.2mg / L after disinfection to induce rapid proliferation of bud; after strengthening the seedling in the B5 basic culture medium, the seedling with no root can be transmitted to a 1 / 2MS culture medium with additional NAA 0.1mg / L, IBA 0.5 mg / L and DSC 1.0 mg / L to induce production of root; the seedling can be transplanted after hardening-seedling and the transplanted survival rate of tube seedling is up to 94 percent to 97 percent. The method provided by the invention is rapid and high efficient and is fit for various cultivars or types of the bupleurum, with low cost, high survival rate of transplanting, easy promotion and good genetic stability. According to market situation, the invention can breed and produce a large number of high-quality cultivated bupleurum plants or provides high-quality seed in short time.

Description

technical field [0001] The invention relates to plant regeneration through tissue culture technology, in particular to a method for rapidly growing seedlings of the medicinal plant Bupleurum bupleuri. Background technique [0002] Bupleurum bupleurum is an important medicinal plant, and it is also a bulk medicinal material used in my country, South Korea, Japan and other countries. Chinese Pharmacopoeia (2000 Edition) included Bupleurum Chinese DC. (also known as hard seedling Bupleurum) and B. scorzonerifolium Willd. (also known as soft seedling Bupleurum Chinese DC.) as medicines The original plant of Bupleurum is used. Bupleurum root is used as medicine. The root contains saikosaponin and volatile oil. It has anti-virus, anti-bacterial endotoxin, anti-inflammatory, anti-tumor, anti-convulsion, antipyretic, lowering blood fat, promoting secretion, protecting liver, and improving immunity. , high medicinal value. [0003] In recent years, the market demand of Bupleurum h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 郝建平徐笑飞
Owner SHANXI UNIV
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