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Quick propagation seedling-breeding method for reducing vitrifaction in multiple times of subculture of rosaceous plant

A technique for subculture and rapid propagation of seedlings, which is applied in the field of multiple subcultures of Rosaceae plants to reduce vitrification and rapid propagation of seedlings, achieving the effects of long cycle, low vitrification ratio, and neat growth of seedlings

Inactive Publication Date: 2012-06-27
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a new rapid propagation seedling method that reduces the occurrence of vitrification in multiple subcultures for the practical problem that Rosaceae plants have vitrified seedlings in the rapid propagation process of tissue culture rapid propagation system

Method used

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  • Quick propagation seedling-breeding method for reducing vitrifaction in multiple times of subculture of rosaceous plant
  • Quick propagation seedling-breeding method for reducing vitrifaction in multiple times of subculture of rosaceous plant
  • Quick propagation seedling-breeding method for reducing vitrifaction in multiple times of subculture of rosaceous plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Effects of different formulas of MS medium on vitrification rate in multiple subcultures

[0026] MS priming medium without any exogenous phytohormones:

[0027] MS+25~35g / L sucrose+5.0g / L agar powder.

[0028] Proliferation medium:

[0029] 1. MS+0.3mg / L 6-BA+25~35g / L sucrose+5.0g / L agar powder;

[0030] 2. MS+0.3mg / L 6-BA+0.2mg / L NAA+25~35g / L sucrose+5.0g / L agar powder;

[0031] 3. MS+0.3mg / L 6-BA+0.5mg / L NAA+25~35g / L sucrose+5.0g / L agar powder;

[0032] 4. MS+0.6mg / L 6-BA+25~35g / L sucrose+5.0g / L agar powder;

[0033] 5. MS+0.6mg / L 6-BA+0.2mg / L NAA+25~35g / L sucrose+5.0g / L agar powder;

[0034] 6. MS+0.6mg / L 6-BA+0.5mg / L NAA+25~35g / L sucrose+5.0g / L agar powder;

[0035] 7. MS+0.9mg / L 6-BA+25~35g / L sucrose+5.0g / L agar powder;

[0036] 8. MS+0.9mg / L 6-BA+0.2mg / L NAA+25~35g / L sucrose+5.0g / L agar powder;

[0037] 9. MS+0.9mg / L 6-BA+0.5mg / L NAA+25~35g / L sucrose+5.0g / L agar powder;

[0038] Rosaceae

[0039]Bean pear, collect the new shoots and young stems of bean ...

Embodiment 2

[0049] Effects of Different Types of Medium on Vitrification Rate in Multiple Subcultures

[0050] This embodiment is based on the rootless shoots obtained in Example 1.

[0051] basic medium

[0052] 1. MS+6-BA 0.6mg / L+NAA 0.2mg / L+3% sucrose+5.0g / L agar powder;

[0053] 2. AS+6-BA 0.6mg / L+NAA 0.2mg / L+3% sucrose+5.0g / L agar powder;

[0054] 3. NN69+6-BA 0.6mg / L+NAA 0.2mg / L+3% sucrose+5.0g / L agar powder;

[0055] 4. WPM+6-BA 0.6mg / L+NAA 0.2mg / L+3% sucrose+5.0g / L agar powder.

[0056] The test method is the same as in the examples, the difference is that the continuous multiplication is cultivated for two generations, and the test data of each generation are recorded in Table 2

[0057] Table 2

[0058]

[0059] It can be seen from Table 2 that different media have certain differences in the proliferation and vitrification of soybean pears. From the results of continuous two-generation proliferation culture, the difference between MS and AS is small. The effect of AS in ...

Embodiment 3

[0061] Effects of two culture methods on vitrification rate in multiple subculture

[0062] This embodiment is based on the rootless shoots obtained by priming culture.

[0063] method one:

[0064] Proliferation medium:

[0065] MS+0.6mg / L 6-BA+0.2mg / L NAA+25~35g / L sucrose+5.0g / L agar powder.

[0066] experiment method:

[0067] The rootless seedlings were inoculated in the proliferation medium, cultured at 25±2°C, 1500-2000Lux, 12-14h / d light, 20-30d for one generation, and continuously multiplied for three generations.

[0068] Method 2:

[0069] Proliferation medium:

[0070] MS+0.6mg / L 6-BA+0.2mg / L NAA+25~35g / L sucrose+5.0g / L agar powder.

[0071] MS priming medium without any exogenous phytohormones:

[0072] MS+25~35g / L sucrose+5.0g / L agar powder.

[0073] experiment method:

[0074] Inoculate the rootless seedlings in the proliferation medium, culture at 25±2°C, 1500-2000Lux, 12-14h / d light, 20-30d for one generation, transfer to the starting medium, rejuvenat...

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Abstract

The invention provides a quick propagation seedling-breeding method for reducing vitrifaction in multiple times of subculture of a rosaceous plant. The method comprises disinfection of explants, induction of buds, sub propagation and rooting induction. The method is characterized in that: in the sub propagation process, test tube seedlings are subjected to rejuvenation culture alternately, and then vigorous test tube seedlings with height of 1 to 3 centimeters are selected and transferred into a rooting culture medium for induced rooting so as to obtain complete test tube plants, wherein a starting culture medium without containing any exogenous plant hormone for rejuvenation is adopted in the rejuvenation culture. The method is quick, efficient, low in cost and convenient for popularization, has good genetic stability and low vitrifaction ratio in the culture process, keeps the propagation coefficient in a high level, and is suitable for tissue culture and quick propagation of the rosaceous plant.

Description

Technical field: [0001] The invention relates to a technique for rapid propagation of plants by tissue culture, in particular to a rapid propagation seedling method for reducing vitrification in multiple subcultures of Rosaceae plants. Background technique: [0002] Tissue culture is to inoculate a part of the plant body on a synthetic medium to make it grow and develop into a new plant according to the predetermined target. Due to the characteristics of short tissue culture cycle, high proliferation rate and year-round production, coupled with the miniaturization of culture materials and test-tube seedlings, a large number of plants can be cultivated in a limited space, and a large number of seedlings can be cultivated in a short period of time. Therefore, in recent years, tissue culture and rapid propagation techniques have been increasingly used in seedling reproduction and production. In addition, with the in-depth research of modern molecular biotechnology, in vitro cu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李晓刚蔺经杨青松王宏伟常有宏
Owner JIANGSU ACAD OF AGRI SCI
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