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Podocarpus extract and its preparation method

A kind of manufacturing method, technology of extract, applied in the field of Podocarpus extract and its manufacture

Active Publication Date: 2010-01-13
TAIYEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the abnormal production of melanin, such as long-term sun exposure, melasma, freckles and age spots and other melanin deposition diseases, also brings a lot of trouble

Method used

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  • Podocarpus extract and its preparation method
  • Podocarpus extract and its preparation method
  • Podocarpus extract and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Please refer to FIG. 2 , which is a flowchart showing the process flow of the Podocarpus extract according to Embodiment 1 to Embodiment 3 of the present invention. First, the extraction step 201 is carried out, which is to use a pulverizer to crush about 8 kilograms of Podocarpus frondosa leaves at room temperature or slightly lower than room temperature, and then immerse the crushed leaves in 80 volume percent methanol aqueous solution middle. Next, the filtering step 203 is performed, which is to filter the 80% aqueous methanol solution containing the pulverized leaves by using gauze and filter paper, and repeat three times to obtain a filtrate. Then, the aqueous methanol solution was removed by concentration under reduced pressure, and the crude extract 204 of Podocarpus macrophylla was obtained.

[0041] Afterwards, the crude extract 204 is subjected to a partial division step 205 using ethyl acetate to obtain an extract 207 of an ethyl acetate layer and an extrac...

Embodiment 2

[0044] Embodiment 2 refers to the method of Embodiment 1. After the extraction and filtration step 201, the filtration step 203, and the partial division step 205, the first separation step 211 is performed, as shown in FIG. 2 . Further, the first separation step 211 of Embodiment 2 is to pass the above-mentioned ethyl acetate layer extract 207 through the first chromatographic column 213, such as Sephadex LH-20 chromatographic column, and rinse with 100 volume percent methanol After stripping, the methanolic extract is collected 214. As described in Example 1, the second part 217 of the methanol extract 214 can be subjected to the second separation step 225, that is, after passing through the above-mentioned reverse phase chromatography column 233, the second extract of Podocarpus macrophylla can be obtained 243.

Embodiment 3

[0046] Embodiment 3 also refers to the method of Embodiment 1. After the extraction and filtration step 201, the filtration step 203, and the partial division step 205, the first separation step 211 is performed, as shown in FIG. 2 . Further, in the first separation step 211 of Embodiment 3, the above-mentioned ethyl acetate layer extract 207 is passed through the first chromatographic column 213, such as a Sephadex LH-20 chromatographic column, and rinsed with 100 volume percent methanol After stripping, the methanolic extract is collected 214. As described in Embodiment 1, the third part 219 of the methanol extract 214 can be subjected to the second separation step 225, that is, through the second chromatographic column 231, such as a Sephadex LH-20 chromatographic column, and first divided into the second chromatographic column. After the fourth part 221 and the fifth part 223, the fourth part 221 and the fifth part 223 are passed through the reverse phase chromatography co...

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Abstract

The invention relates to a Podocarpus revocation and it's making method. It uses the organic solution and the gel chromatography method to draw up the leaf of the Podocarpus to obtain the Podocarpus revocation, wherein the Podocarpus revocation is at least one bis-flavonoid. The Podocarpus revocation has the effect of anti-skin aging.

Description

technical field [0001] The invention relates to a Podocarpus spp. extract and a manufacturing method thereof, in particular to a Podocarpus spp. extract containing Biflavonoid compounds and a manufacturing method thereof. Background technique [0002] The skin is the largest organ of the human body, and it is also the first line of defense between the human body and the outside world. The skin has many functions, the most important of which is to protect the human body, prevent the invasion of germs, and prevent excessive loss of water in the body. And the skin contains melanin, which is brown to black. When the skin is exposed to sunlight, the color of melanin will be deepened and the amount will also increase, which can prevent ultraviolet rays from penetrating into the body. In addition, the skin also has the functions of excretion, temperature regulation, and sensation. [0003] Skin aging is an inevitable process. Aging skin will produce various changes in tissue mor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30A61P39/06A61P43/00A61P17/00
Inventor 李美贤郑可大陈世辉吕思洁
Owner TAIYEN BIOTECH
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