Method for preparing glycosides in high purity

A rubusoside, high-purity technology, applied in the field of preparation of rubusoside, can solve the problems of low utilization rate, inconvenient use, resource waste, etc., and achieve the effect of promoting resource utilization, simple extraction method and high purity

Inactive Publication Date: 2007-07-25
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, people's utilization of sweet tea is mainly to soak the leaves as beverages, or as primary products made from extracts. Glycosides for sale

Method used

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  • Method for preparing glycosides in high purity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 100g of dried sweet tea leaves, crush them, put them into a round bottom flask, add water at a solid-to-liquid ratio of 1:10, boil for 60 minutes, cool and filter, boil for the second and third times for 30 minutes, and combine the filtrates. Pass the filtrate through a macroporous adsorption resin column at a speed of 10ml / min. The specification of the adsorption column is 4.0×60cm. After the adsorption is completed, first wash the column with distilled water until the effluent is colorless, then elute with 75% ethanol, and the eluent After recovering the alcohol by evaporation, add 1000ml of water, filter, and perform column chromatography on the filtrate to remove impurities with a column size of 4.0×60cm, collect the column flow, concentrate, vacuum or spray dry to obtain a solid containing 81% rubusoside Powder sample 3.01g, then add 8 times the volume of absolute alcohol to the solid powder, heat it in a water bath at 70°C until it is completely dissolved, fil...

Embodiment 2

[0020] Take 100g of dried sweet tea leaves, crush them and put them into a round-bottomed flask, add 50% ethanol solution with a solid-to-liquid ratio of 1:10, boil for 60 minutes, cool and filter, boil the second and third times for 30 minutes to combine the filtrate, and put it into distillation The tank is heated to a boil and the ethanol is recovered. All the other steps are the same as in Example 1, that is, 1.5 g of white crystal high-purity rubusoside containing 98.2% of rubusoside is obtained

Embodiment 3

[0022] Take 100g of dried sweet tea leaves, crush them, put them into a round-bottomed flask, add a mixed solvent of water and ethanol, (solid-to-liquid ratio 1:10), boil for 60 minutes, cool and filter, boil for the second and third times for 30 minutes to combine the filtrate, filter, and filter the filtrate Carry out alumina column chromatography, the column specification is 4.0 * 60cm, other steps are the same as Example 1, can obtain the white crystal high-purity rubusoside 1.4g containing 98.5% rubusoside.

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Abstract

This invention discloses a method for extracting high-purity glucoside of Rubas suavissimus. The method comprises: extracting Rubas suavissimus with water or mixed solvents of water and ethanol under refluxing, adsorbing by resin, eluting, removing impurities by column chromatography, concentrating, drying to obtain powdery crude product, dissolving with absolute ethanol by heating, filtering, and crystallizing to obtain high-purity glucoside. The method is simple, and the product has such advantages as high purity and high sweetness. The glucoside can be used to produce high-sweetness healthcare products or drugs with low caloric value.

Description

Technical field: [0001] The invention relates to a preparation method of rubusoside, in particular to a method for extracting rubusoside from sweet tea leaves and removing impurities by column chromatography to prepare high-purity rubusoside. Background technique: [0002] Sweet tea (Rubas Suavissimus S.Lee.) is a perennial deciduous shrub with thorns in the Rosaceae Rubus genus. It is a rare wild sweet plant unique to Guangxi. One of its main sweet ingredients is Steviol. Tetracyclic diterpene glycosides combined with glucose, also known as rubusoside, has a molecular formula of C32H50O13. The sweetness of rubusoside is about 300 times that of sucrose. It is a natural sweetener with low calorific value and high sweetness. It has the functions of lowering blood pressure and blood sugar, promoting metabolism, and treating hyperacidity. It is extremely safe to use through toxicological experiments. At present, people's utilization of sweet tea is mainly to soak the leaves as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/20C07H15/256
Inventor 陈全斌义祥辉何星存苏小建张巧云
Owner GUANGXI NORMAL UNIV
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