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Snow lotus polyploid inducing process

A technology of polyploid and snow lotus, applied in horticultural methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of explants poisoning colchicine, growth stagnation, and slow growth of regenerated plants, etc. Achieve significant polyploid characteristics, promote direct germination, and induce high survival rates

Inactive Publication Date: 2007-08-22
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0015] Problems to be solved in polyploid induction: (1) Toxic effect of colchicine: colchicine is highly toxic and has toxic effect on explants. The toxic effect of colchicine is mainly reflected in: inducing material death, inhibiting root growth growth and seed germination, inhibition of adventitious bud differentiation, slow growth or stagnation of regenerated plants
[0016] At present, a variety of medicinal plants in China have obtained polyploid plants through various methods, but there is no report on the induction of polyploid in Saussurea japonicus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The seeds of Saussurea involucrata (S.involucrata) were first surface-sterilized with 75% ethanol for 20sec, and then with 0.1% - HgCl 2 The solution was sterilized for 7 min, followed by 15% H 2 o 2 The solution was sterilized for 30 minutes, and the sterilized seeds were rinsed 4 times with sterilized water, and then sowed on the MS medium, and 10 seeds were sown in each bottle of MS medium, and after 7 days, the seeds germinated to produce aseptic seedlings;

[0036] Select 30 strains of aseptic seedlings growing for 30 days, completely excise their roots and leaves, and only keep the stem part;

[0037] The stem part of the sterile seedlings retained after cutting was inoculated in a mixture of 2% dimethyl sulfoxide (DMSO) and 100 mg L colchicine. -1 On the budding induction solid medium, induction 2d, the budding induction solid medium formula is MS+6-BA 0.1mg·L -1 +TDZ0.3mg·L -1 ;

[0038] After 3 days of stem induction of sterile seedlings, transfer to a so...

Embodiment 2

[0041] The snow lotus seeds were first surface sterilized with 75% ethanol for 30 sec, and then 0.1% HgCl 2 The solution was sterilized for 10 min, followed by 15% H 2 o 2 The solution was sterilized for 40 minutes, and the sterilized seeds were rinsed 5 times with sterilized water, and then sowed on the MS medium, and 12 seeds were sown in every bottle of MS medium, and after 8 days, the seeds of the snow lotus germinated to produce aseptic seedlings;

[0042] Select 30 strains of aseptic seedlings growing for 35 days, completely excise their roots and leaves, and only keep the stem part;

[0043] Inoculate the stem part of the sterile seedlings retained after cutting with 3% dimethyl sulfoxide, and the concentration of colchicine is 300 mg·L -1 On the budding induction medium, induction 3d, the formula of budding induction solid medium is MS+6-BA 0.3mg·L -1 +TDZ0.6mg·L -1 ;

[0044] After 3 days of stem induction of sterile seedlings, transfer to colchicine-free MS+6-BA...

Embodiment 3

[0047] The seeds of snow lotus (S.involucrata) were first sterilized with 75% ethanol for 35 sec, and then with 0.1% HgCl 2 The solution was sterilized for 10 min, followed by 15% H 2 o 2 The solution was sterilized for 45 minutes, and the sterilized seeds were rinsed 6 times with sterilized water, and then sowed on the MS medium, and 13 seeds were sown in every bottle of MS medium, and the seeds of Saussurea chinensis germinated after 9 days to produce aseptic seedlings;

[0048] Select 30 strains of aseptic seedlings growing for 40 days, completely excise their roots and leaves, and only keep the stem part;

[0049] The stem part of the sterile seedlings retained after cutting was inoculated with 4% dimethyl sulfoxide, and the concentration of colchicine was 400 mg·L -1 On the budding induction medium of , induction 3d, the formula of budding induction solid medium is MS+6-BA 0.6mg·L -1 +TDZ0.8mg·L -1 ;

[0050] After 3 days of stem induction of sterile seedlings, trans...

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Abstract

The snow lotus polyploid inducing process includes the first germinating snow lotus seed to generate aseptic seedling, the subsequent cutting off the roots and leaves of the aseptic seedling, inoculating the stem of the aseptic seedling first to culture medium with dimethyl sulfoxide and colchicina and then to culture medium without dimethyl sulfoxide and colchicina to induce germination so as to obtain new sprout and new distinctly different plant, and finally chromosome detection on the stem apex growing point of the aseptic seedling to determine ploidy of the chromosome and obtain snow lotus polyploid plant.

Description

technical field [0001] The invention relates to a method for inducing Saussurea polyploid Background technique [0002] Xinjiang Snow Lotus: Saussurea involucrata Kar et Kir. is an alpine herbaceous plant of the genus Asteraceae. , in the Tianshan Mountains, it mainly grows in alpine meadows at an altitude of 2400-4100m, rock crevices on rocky beaches, etc., and the living conditions are harsh. Modern pharmacological studies have shown that Saussurea sauraceae has anti-inflammatory, analgesic, anti-cancer, dilating blood vessels, lowering blood pressure, scavenging free radicals, anti-fatigue, terminating pregnancy and shrinking the uterus. Saussurea sauraceae secondary metabolites have complex components, and the compounds with medicinal activity are mainly concentrated in flavonoids, alkaloids and polysaccharides, which are rare and valuable medicinal materials in Chinese traditional medicine. Because the medicinal and health effects of snow lotus are widely known, the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01C1/00A01H4/00A01G31/00C12Q1/68C12N5/04
Inventor 张蜀敏王晓军郝秀英徐琴
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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