Eugenol, osthol complex biological bactericide
A technology for compounding fungicides and ostholes, applied in the directions of fungicides, biocides, biocides, etc., can solve the problems of high cost, no relevant reports and applications, achieve low toxicity, overcome the tendency to produce antibacterial drugs, etc. performance, cost reduction
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Embodiment 1
[0016] Example 1: Indoor formulation screening of compounded eugenol and osthole
[0017] 1. Drugs to be tested
[0018] A. 99.0% eugenol was purchased from Nanjing Fengze Native Animal Products Co., Ltd., dissolved in 70% ethanol, and prepared as a 10000 μg / ml mother liquor for later use.
[0019] B. 94.7% osthole, purchased from Longquan Hi-Tech Biological Products Company in Chengdu, Sichuan, dissolved in 70% ethanol, and prepared as a 10000 μg / ml mother solution for future use.
[0020] 2. Test strains
[0021] Rice sheath blight (Rhizoctonia solani), rape sclerotiorum (Sclerotinia sclerotiorum), tomato gray mold (Botrytis cinerea) (all provided by the Institute of Food Quality Safety and Testing, Jiangsu Academy of Agricultural Sciences).
[0022] 3. Test method
[0023] Adopt mycelia growth rate measurement method: the test is established with 9 treatments, i.e. eugenol, osthole, 5 different proportions of eugenol and osthole, solvent control and blank control, and th...
Embodiment 2
[0039] Embodiment 2: field efficacy test of eugenol and osthole compound fungicide
[0040] Field test of eugenol and osthole compound fungicide for controlling strawberry powdery mildew (the test program was designed according to the "Pesticide Field Efficacy Test Guidelines (2)"):
[0041] 1. Experimental Design and Arrangement
[0042] 1. Test crops and control objects.
[0043] Test crops: Strawberry, the variety is Fengxiang.
[0044] Control object: Strawberry powdery mildew, the pathogen is Sphaerotheca pannosa.
[0045] 2. Drugs to be tested
[0046] Test drug:
[0047] 20% eugenol microemulsion, Jiangsu Academy of Agricultural Sciences, the effective concentration is 300μg / ml.
[0048] 1% osthole water emulsion, Jiangsu Academy of Agricultural Sciences, effective concentration is 40μg / ml.
[0049] 22.5% eugenol·osthole microemulsion (active ingredient: 20% eugenol + 2.5% osthole), Jiangsu Academy of Agricultural Sciences, the effective concentration is 200 μg / ml...
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