Method for extracting small molecule active peptides from scapharca subcrenata

Abstract

The invention relates to an method for extracting small molecule active polypeptide, consisting of shuck off the arca subcrenata shell, washing, homogenating, adding distilled water for a low temperature extraction, flocculating, centrifugation, removing the dregs, taking supernatant fluid, doing a gel column chromatography after freeze drying the supernatant fluid, collecting respective peak, then doing an ion-exchange chromatography to achieve the separated purification, collecting the active peak, desalting by gel column after freeze-dry to get a highly purified natural active polypeptide, going on purifying the produced small molecule peptide by reversed-phase column to get the active peptide with a purity more than 95%. The extraction progress effectively protects the activity of the peptide. The active peptide has an obvious anti-tumor activity with a tumor-inhibiting rate to 52% proved by anti-tumor experiments in vitro and can be used for the exploiture of new-type anti-tumor drug.

Description

Technical field:

[0001] The invention relates to a method for extracting small-molecule active peptides from cockles, in particular to separating and purifying the active peptides from cockles by column chromatography, and belongs to the technical field of marine natural product extraction and medicine. Background technique:

[0002] Scapharca subcrenata (Arca subcrenata Lischke; Scapharca subcrenata) belongs to the phylum Molluscs, Class Phyllobranches, Order Dendonta, and Scapharca family. It is one of the abundant marine organisms in my country and has a long history of medicinal use. Its shell is named corrugated fruit, which has been recorded in past materia medica. It has the functions of reducing phlegm, softening hardness, reducing acid and relieving pain. The cockle meat is a soft part, which is mostly contained in dietotherapy herbal medicines. The clam as a whole is used as medicine, which has the function of shell and meat. It can nourish the blood and warm the m...