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Method for extracting small molecule active peptides from scapharca subcrenata

A technology of active peptides and small molecules, applied in peptide sources, animal/human peptides, anti-tumor drugs, etc.

Inactive Publication Date: 2007-09-19
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

The above patent documents describe in detail the extraction process and pharmacological activity of active substances in cockles, but more in-depth research on active substances, such as structural analysis and research on physical and chemical properties, has not been reported.

Method used

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Embodiment Construction

[0008] The present invention will be further described below in conjunction with the examples.

[0009] Shell the cockles, take the cockle meat, wash with water at 4°C, homogenate, add distilled water at a low temperature (4°C) for extraction for 2 hours according to the weight ratio of 1:1, flocculate, centrifuge to remove slag, take the supernatant, and After the supernatant was lyophilized, it was subjected to gel column chromatography with Sephadex G25 (0.03mol / lNH 4 HCO 3 elution buffer), after collecting the peaks respectively, take the peaks with biological activity and then use anionic Sephadex DEAE-Sephadex A25 ion exchange column chromatography (PH8.5 Tris-HCl buffer, 0 ~ 0.03mol / NaCl Gradient elution), the active peaks were collected, and after freeze-drying, desalted with Sephadex G15 gel column to obtain natural small molecule active peptides with high purity. The active peptide is a naturally extracted substance without any chemical modification, and the extrac...

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Abstract

The invention relates to an method for extracting small molecule active polypeptide, consisting of shuck off the arca subcrenata shell, washing, homogenating, adding distilled water for a low temperature extraction, flocculating, centrifugation, removing the dregs, taking supernatant fluid, doing a gel column chromatography after freeze drying the supernatant fluid, collecting respective peak, then doing an ion-exchange chromatography to achieve the separated purification, collecting the active peak, desalting by gel column after freeze-dry to get a highly purified natural active polypeptide, going on purifying the produced small molecule peptide by reversed-phase column to get the active peptide with a purity more than 95%. The extraction progress effectively protects the activity of the peptide. The active peptide has an obvious anti-tumor activity with a tumor-inhibiting rate to 52% proved by anti-tumor experiments in vitro and can be used for the exploiture of new-type anti-tumor drug.

Description

Technical field: [0001] The invention relates to a method for extracting small-molecule active peptides from cockles, in particular to separating and purifying the active peptides from cockles by column chromatography, and belongs to the technical field of marine natural product extraction and medicine. Background technique: [0002] Scapharca subcrenata (Arca subcrenata Lischke; Scapharca subcrenata) belongs to the phylum Molluscs, Class Phyllobranches, Order Dendonta, and Scapharca family. It is one of the abundant marine organisms in my country and has a long history of medicinal use. Its shell is named corrugated fruit, which has been recorded in past materia medica. It has the functions of reducing phlegm, softening hardness, reducing acid and relieving pain. The cockle meat is a soft part, which is mostly contained in dietotherapy herbal medicines. The clam as a whole is used as medicine, which has the function of shell and meat. It can nourish the blood and warm the m...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K14/435A61P35/00
Inventor 韩玉谦冯晓梅赵志强蒋新管华诗
Owner OCEAN UNIV OF CHINA
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