Method for preparing active polypeptide from Arca subcrenata lischke
A technology of active peptides and cockles, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc.
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[0008] The present invention will be further described below in conjunction with the examples.
[0009] Shell the cockles, take the cockle meat, wash with water at 4°C, homogenate, add distilled water at a low temperature (4°C) for extraction for 2 hours at a weight ratio of 1:1, flocculate, centrifuge to remove slag, take the supernatant, and After the supernatant was lyophilized, it was subjected to gel column chromatography with Sephadex G25 (0.03mol / 1NH 4 HCO 3 elution buffer), after collecting the peaks respectively, take the peaks with biological activity and then use anionic Sephadex DEAE-Sephadex A25 ion exchange column chromatography (PH8.5 Tris-HC1 buffer, 0 ~ 0.03mol / NaCl Gradient elution), the active peaks were collected, and after freeze-drying, desalted with Sephadex G15 gel column to obtain natural small molecule active peptides with high purity. The active peptide is a naturally extracted substance without any chemical modification, and the extraction process...
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