Nasal cavity immunity composite adjuvant for avian influenza inactivation antigen
A technology of inactivating antigen and immune compounding, which is used in antiviral agents, antibody medical ingredients, medical preparations containing active ingredients, etc., to achieve the effects of improving whole body humoral immunity, improving immune effect, and reducing the stress response of chickens.
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[0036] Sodium cholate, CpG DNA and avian influenza H5N2 inactivated antigen were combined with SPF chickens to nasally immunize SPF chickens. Each chicken (approximately 700 g): CpG 50μg+sodium cholate 20μg, to study the effect on the local and systemic immune levels of the chicken respiratory tract. First of all, the first phase of the project was tested. Apply CpG DNA separately according to plan (see references for extraction methods: Cui Yibang, He Kongwang, Lu Chengping, Guo Aizhen. The immune enhancement effect of bacterial CpGDNA on chickens inoculated with avian influenza virus H9 subtype inactivated vaccine. Chinese Journal of Veterinary Medicine 2005 25(2): 135~ 137). Sodium cholate is a mucosal immune adjuvant combined with avian influenza inactivated antigen (purchased from the Animal Quarantine Institute of the Ministry of Agriculture).
[0037] The above nasal mucosal immune complex adjuvants were combined with avian influenza inactivated antigens to immunize healthy...
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