Method for producing biological energy source substance by using chitin

A bio-energy, chitin technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unutilized chitin and offshore pollution.

Inactive Publication Date: 2008-02-27
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of chitin is treated as waste due to unreasonable utilization, causing offshore pollution

Method used

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  • Method for producing biological energy source substance by using chitin
  • Method for producing biological energy source substance by using chitin
  • Method for producing biological energy source substance by using chitin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0057] 1. Pretreatment of chitin by chemical method

[0058] Soak the raw material in a solution containing 0.8-1.2M hydrochloric acid for 1-3 hours, and treat it two to three times to remove calcium carbonate and inorganic salt contained in the raw material. Then treat it with 40-70g / L NaOH solution for 2 hours, and treat it three times to remove the protein contained in the raw material. After cleaning, the pretreatment process of raw materials is completed.

[0059] 2. Taking chitin hydrolase as an example to illustrate the acquisition, production and catalytic conversion methods of key enzymes in chitin conversion.

[0060] 2.1 Acquisition of the chitin hydrolase gene from the Asian corn borer

[0061] Design primers based on homologous sequences:

[0062] CF1: 5'-GACATRTTCCAYATGGGMGG-3'

[0063] CR1: 5'-TCCGMCTGCTCBGACCACAG-3'

[0064] The total RNA was extracted from the corn borer using the TaKaRa RNAiso Reagent kit (Fig. 1), and after denaturation, it was reverse-...

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Abstract

The present invention provides a method capable of utilizing modern bio-technical process to implement production of biological energy source substances by using chitin-natural L-acetylglucosamine multipolymer. It is characterized by that said method includes the following steps: (1), pretreatment of chitin raw material: (2), adopting modern bio-technical process, utilizing natural or recombinant or artificially-engineered chitin metabolic enzyme system and utilizing internal conversion or external conversion method to make chitin be converted into biological energy source precursor substance 6-phosphofructose; and (3), using 6-phosphofructose as carbon source, adopting biological conversion method to produce the biological energy source substances of alcohol, etc.

Description

Technical field: [0001] The invention belongs to the field of bioenergy, and relates to a method for producing bioenergy materials from natural N-acetylglucosamine polymers such as chitin by means of modern biotechnology methods. Background technique: [0002] One of the biggest challenges in the world in the 21st century is how to continuously provide energy needed for economic development and people's lives. Energy security has been raised to a strategic level by various countries. Due to the shortage of fossil fuels and pollution problems, people have long focused on the development of renewable energy, and the development and utilization of biomass energy has received great attention (Arthur J.Ragauskas et al., 2006). The European Commission plans to use renewable fuels to replace 20% of fossil fuel usage in 2020, and their mid-term goal is to reach 5.75% in 2010; the 2005 US Energy Policy Act requires the US to use 7.5 billion gallons of fuel ethanol by 2012 ( About 22...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P7/06C12P21/00C12P3/00C12R1/19C12R1/425C12R1/465C12R1/63
CPCY02E50/17Y02E50/10
Inventor 杨青屈明博刘田杨君
Owner DALIAN UNIV OF TECH
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