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Gastrodia elata medicinal materials discrimination method

A technology of gastrodia elata and medicinal materials, applied in the field of identification of gastrodia elata medicinal materials

Inactive Publication Date: 2008-03-26
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

How to efficiently identify the quality and authenticity of Gastrodia elata, especially the effective method for identifying the quality of traditional Chinese medicine that is internationally recognized today, has not yet been reported

Method used

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  • Gastrodia elata medicinal materials discrimination method
  • Gastrodia elata medicinal materials discrimination method
  • Gastrodia elata medicinal materials discrimination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The chemical fingerprint of Gastrodia elata standard was determined by optimized extraction method and separation and detection conditions. That is, accurately weigh 0.5g of 80-mesh powder from Gastrodia elata sample, put it into a Soxhlet extractor, then add 70% ethanol, put it in a water bath at 98°C for 20 minutes, treat it with an ultrasonic cell disruptor for 25 minutes, and weigh it after ultrasonic treatment , make up the lost weight with the corresponding solvent. Repeat the above process once. After filtering, concentrating and drying, fix the solution to 10ml with 10% acetonitrile solution. With high performance liquid chromatography (Agilent1100, made in the United States), Eclipse, XD13-C 8Chromatographic column, 10% acetonitrile solution as mobile phase, 25°C column temperature, 20μl injection volume, 1ml / min flow rate, 221nm wavelength light to determine the chemical fingerprint of Gastrodia elata standard. The results are shown in Figure 3.

Embodiment 2

[0038] Take wild gastrodia elata from Sichuan, wild gastrodia elata from Northeast, artificial gastrodia from Hubei, artificial gastrodia from Shaanxi, artificial gastrodia from Guizhou, wild gastrodia from Yunnan, and artificial gastrodia from Yunnan, and use the method and steps in Example 1 to obtain their chemical fingerprints, as shown in the figure 4-11.

[0039] The chemical fingerprints of Gastrodia elata from different regions have different characteristics and different numbers of peaks. However, they have 5 common peaks (peak 1, 2, 4, 5 and 7) at the same position, especially a very stable gastrodin characteristic peak (peak 4). According to the retention time (1.921min) of gastrodin reference substance [20] , the peaks with retention times between 1.908 and 1.939min (obtained from 22 samples) in the spectrum are gastrodin peaks, and the maximum deviation between the gastrodin peaks of various Gastrodia elata and the Gastrodia elata standard is 1.9%. Gastrodia ela...

Embodiment 3

[0047] Using the method and steps of Example 1, the chemical fingerprint of the counterfeit Gastrodia elata potato was determined (see Figure 12). Comparing the chemical fingerprints of the counterfeit products with those of the standard Gastrodia elata and other true Gastrodia elata, it can be seen that there are only 5 peaks in the pseudogastrodia elata potato. Their retention times are respectively: 1.356min (peak 1), 1.558min (2), 1.955 (3), 2.226 (4), 3.122 (5), and the deviation with the corresponding peak of Gastrodia elata standard substance is respectively: 4.71% ( For Tianma peak 1), 3.25% (for 2), 3.5% (for 4)%, 30.9% (for 5) and 29.3% (for 7). There is not a common peak between pseudo Gastrodia elata and true Gastrodia elata. In particular, it lacks the characteristic peak of gastrodin with large area (between 1.908 and 1.939 min) that all Gastrodia elata samples have. According to these characteristics of fingerprints, it is easy to distinguish the authenticity ...

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Abstract

An identification method of the rhizome gastrodiae includes the below processes: (1) setting the standard chemical fingerprint atlas of rhizome gastrodiae: a. putting the rhizome gastrodiae standard sample into the sorbitic extractor; b. adding the ethanol and putting in the water bath for hot reflux, treating it by the ultrasonic cell knapper and weight, then to loss weight by the corresponding solvent; repeating it again; after filtration, concentration and drying, it uses the 10% acetonitrile to constant volume; c. using the HPLC, Eclipse, XD13-C8 chromatographic column, the 10% acetonitrile solution is as the mobile phase, the light of 221+-1nm wavelength is as the detection light source to determine the chemical fingerprint atlas of rhizome gastrodiae of rhizome gastrodiae standard sample; which has seven evidence peaks, the peak with 1.920min holding time is the rhizome gastrodiae element peak, the rhizome gastrodiae element peak of the other rhizome gastrodiae has the big warp of 1.9% with the standard sample. (2) Determining the chemical fingerprint of the detected sample and the dummy sample according to the progress (1) and comparing to identify.

Description

technical field [0001] The invention relates to a construction method of gastrodia elata chemical fingerprint and its application in gastrodia elata identification. Background technique [0002] Gastrodia elata is one of the four precious Chinese medicinal materials (cordyceps sinensis, ginseng, pilose antler) in China. It has the effect of calming the liver, calming wind and relieving spasm. In recent years, research has found that Gastrodia elata also has the functions of increasing intelligence, strengthening the brain, treating senile dementia, and delaying aging. , Anti-inflammation, enhance immune function, lower blood pressure, adjust heart rate, increase cardiac output, etc. to regulate cardiovascular effects, and the side effects are very small. The main active ingredient of gastrodia elata is gastrodin. The level of gastrodin content is an important indicator to judge the quality of Gastrodia elata. As wild Gastrodia elata is becoming less and less, incidents of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 陶钧陶垚陈平胡晓刘培陈贵恒刘苏娟张长庚
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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