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Method for analyzing a blood sample and apparatus and reagent for implementing said method

A technology for blood samples and analysis solutions, which is applied to the equipment and reagents it performs, in the field of blood sample analysis, to achieve the effect of simplifying the analysis equipment

Active Publication Date: 2008-05-21
C2 DIAGNOSTICS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Also, it requires the use of multiple syringes and solvents

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  • Method for analyzing a blood sample and apparatus and reagent for implementing said method
  • Method for analyzing a blood sample and apparatus and reagent for implementing said method
  • Method for analyzing a blood sample and apparatus and reagent for implementing said method

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Embodiment Construction

[0179] Fig. 1 schematically shows a unique dilution and analysis tank 1, which can provide a blood sample 2 to be analyzed, a diluent 3 and a reagent 4 to form an analysis solution together. This tank 1 is equipped with means 5 for measuring the level of hemoglobin in said analysis solution by photometry, and means 6 for measuring the resistance of said analysis solution in order to calculate the total number of leukocytes. Also provided are some means for withdrawing a portion of the analysis solution from the analysis tank 1 and for injecting it into an optical tank 7 equipped with an optical measuring device 8 (such as a flow cytometer) for analysis leukocyte. According to selected embodiments, there are also provided means for withdrawing a portion of the sample pre-solution consisting of blood and diluent and injecting it into the counting and dilution tank 9 equipped for measuring said portion Solution resistant device 10 for counting red blood cells and platelets. The...

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Abstract

The invention concerns a method for analyzing a blood sample, including the following steps: forming in a single so-called diluting and analyzing vessel an analysis solution containing said blood sample, a diluent, and at: least one compound for lysing the red blood cells; at least one compound for stabilizing hemoglobin in the form of a chromogenic complex; measuring on said analysis solution in said vessel after lysis of the red blood cells the hemoglobin level by spectrophotometry; and drawing an appropriate amount of said analysis solution in said vessel whereon a leukocyte differentiation is performed by an optical means. The invention is characterized in that the analysis solution further contains at least one compound to protect the white blood cells, enabling at least four main leukocyte subpopulations to be discriminated. The invention also concerns a hematological apparatus for implementing said method.

Description

technical field [0001] The invention relates to a method for the analysis of a blood sample, as well as devices and reagents for its execution. More specifically, the invention relates to the field of automated analysis of blood samples. Background technique [0002] Analysis by blood samples is usually devoted to determining: [0003] - the total number of white blood cells; [0004] - More specifically, the number of white blood cells by sub-population (basophils, eosinophils, neutrophils, monocytes and lymphocytes); [0005] - the number of red blood cells and platelets; and [0006] - Hemoglobin level. [0007] Various analytical techniques are known, in particular: [0008] - Hemoglobin determination is performed after erythrocyte lysis, i.e. destruction of the red blood cell membrane, and is determined by spectrophotometric measurement of hemoglobin released in the culture medium; hemoglobin determination also requires hemoglobin in a complex form (oxyhemoglobin o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72G01N33/50
CPCG01N33/721G01N33/5094G01N2015/008G01N2015/1006G01N2015/1486G01N15/1459G01N15/1404Y10T436/25Y10T436/107497Y10T436/108331Y10T436/101666Y10T436/10Y10T436/25625G01N33/49G01N33/50G01N33/48
Inventor H·钱普赛克斯J·吉劳德
Owner C2 DIAGNOSTICS
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