SNP markers in leukocyte differentiation antigen cd34 gene related to porcine resistance to PRRS and its application

Abstract

The present invention relates to a SNP marker related to porcine anti-PRRS disease in the leukocyte differentiation antigen CD34 gene and an application thereof. The SNP marker related to porcine anti-PRRS disease is located on the porcine leukocyte differentiation antigen CD34 gene, and the SNP marker is The nucleotide sequence is shown in SEQ ID NO.1, wherein, the pigs to be tested whose 256bp and 260bp bases are A are susceptible to PRRS pigs, and the two bases are G. The tested pigs were resistant to PRRS. The molecular genetic markers provided by the present invention are not limited by the age and sex of pigs, and can be used for early breeding of PRRS-resistant pig varieties, and can be accurately screened even when pigs are just born, which can greatly speed up PRRS resistance The breeding process of pigs; the screening method is accurate, the operation is simple, the cost is low, and the efficiency is high.

Description

technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to the SNP marker in the leukocyte differentiation antigen CD34 gene related to pig resistance to PRRS and its application. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), commonly known as "PRRS", is caused by porcine reproductive and respiratory syndrome virus (PRRSV), a member of the family Arteriviridae of the order Nidoviridae. There are equine arteritis virus, murine lactate dehydrogenase virus, and simian hemorrhagic fever virus. The disease mainly causes reproductive and respiratory symptoms of pigs, manifested as interstitial pneumonia and mummified sow abortion, which causes huge economic losses to the pig industry all over the world every year. A high fever outbreak occurred in China in 2007. The disease spread rapidly across the country. More than 2 million pigs were infected and 400,000 pigs died. The disease...

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Problems solved by technology

PRRSV mainly infects alveolar macrophages of pigs. Now studies have shown that after PRRSV infection, PRRSV infection in innate immunity cannot elicit an effective type I interferon response, humoral immunity cannot produce effective neutralizing antibodies in time, and antibody-dependent Virus enhancement effect eventually leads to immunosuppression and persistent infection. Due to the extremely high mutation rate of the virus, traditional vaccine methods cannot effectively control the disease. Many studies are devoted to finding new methods such as RNAi and interferon to target the virus. Used to make up for the shortcomings of traditional methods

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