Porcine anti-porcine reproductive and respiratory syndrome related SNP label in leukocyte differentiation antigen CD34 gene and application thereof

Abstract

The invention relates to a porcine anti-porcine reproductive and respiratory syndrome (PRRS) related SNP label in a leukocyte differentiation antigen CD34 gene and application thereof. The porcine anti-porcine reproductive and respiratory syndrome related SNP label is located on the porcine leukocyte differentiation antigen CD34 gene. An SNP labeled nucleotide sequence is shown as SEQ ID NO.1, wherein a to-be-tested pig with A basic groups at 256bp and 260bp of the sequence belongs to a PRRS susceptible variety, and a to-be-tested pig with G basic groups at the two sites belongs to an anti-PRRS variety. The molecular genetic label provided by the invention is not restricted by porcine age, gender and the like, can be used for early breeding of the anti-PRRS pig variety, and even can realize accurate screening of newborn pigs, thus greatly accelerating the breeding process of anti-PRRS pigs. The screening method is accurate, and has the advantages of simple operation, low cost, and high efficiency.

Description

technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to the SNP marker in the leukocyte differentiation antigen CD34 gene related to pig resistance to PRRS and its application. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), commonly known as "PRRS", is caused by porcine reproductive and respiratory syndrome virus (PRRSV), a member of the family Arteriviridae of the order Nidoviridae. There are equine arteritis virus, murine lactate dehydrogenase virus, and simian hemorrhagic fever virus. The disease mainly causes reproductive and respiratory symptoms of pigs, manifested as interstitial pneumonia and mummified sow abortion, which causes huge economic losses to the pig industry all over the world every year. A high fever outbreak occurred in China in 2007. The disease spread rapidly across the country. More than 2 million pigs were infected and 400,000 pigs died. The disease...

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Problems solved by technology

PRRSV mainly infects alveolar macrophages of pigs. Now studies have shown that after PRRSV infection, PRRSV infection in innate immunity cannot elicit an effective type I interferon response, humoral immunity cannot produce effective neutralizing antibodies in time, and antibody-dependent Virus enhancement effect eventually leads to immunosuppression and persistent infection. Due to the extremely high mutation rate of the virus, traditional vaccine methods cannot effectively control the disease. Many studies are devoted to finding new methods such as RNAi and interferon to target the virus. Used to make up for the shortcomings of traditional methods

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