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Biocompatible composition for replacing/regenerating tissues

A technology of biocompatibility and composition, applied in tissue regeneration, drug delivery, coating, etc., which can solve the problems of insufficient mechanical properties of biocompatibility

Inactive Publication Date: 2008-05-28
朱塞佩·卡尔沃萨
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But most of them suffer from insufficient biocompatibility and / or mechanical properties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0060] Under gentle stirring, add 5 ml of 4% sodium alginate solution (composition 1) in medium (D-MEM) and 5 ml of 0.4% hyaluronic acid solution in medium (D-MEM) ( To the mixture of composition 2) was added 5 ml of a solution (composition 3) prepared according to the method in which 300 mg of gelatin microparticles were suspended in medium (D-MEM).

[0061] Finally, with gentle mixing, 5 ml of a cell suspension (dermal fibroblasts) (200×10 5 cells / ml).

[0062] The above process was carried out under sterile conditions at room temperature. The process of crosslinking the alginate and thereafter obtaining a three-dimensional hydrogel matrix embedded with living cells begins about 15 minutes after the addition of composition 3.

[0063] The biodegradable microparticles of composition 3 of the cross-linking agent used to obtain the controlled release of sodium alginate can be prepared, for example, as described below.

[0064] 1. Dissolve 0.2g of gelatin in 20ml of distilled w...

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PUM

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Abstract

A biocompatible composition for replacing / regenerating articular tissues, in particular tissues of the nucleus pulposus but also not articular tissues such as bony tissues, providing a hydrogel matrix comprising a first and a second natural polymer, cells adapted to regenerate the tissue of the disc core, a cross linking agent suitable to cross-link at least one of said natural polymers in situ, thus giving to the biocompatible composition an appropriate resistance, and molecules adapted to stimulate the growth and / or the differentiation of cells, wherein one of the two natural polymers is sodium alginate. In a particular exemple, the above described composition before the cross-linking step has a starting density such that it is injectable. In particular, the cross linking action on sodium alginate is caused by a cross linking agent contained in biodegradable polymeric particles, released in a controlled way from natural polymers, for example gelatin, or starting from synthetic polymers, for example polylactic acid or copolymers of acid lactic-glycolic acid.

Description

technical field [0001] The present invention relates to the field of medicine / surgery, more specifically, the present invention relates to biocompatible compositions for replacing / regenerating biological tissues. [0002] Specifically (but not exclusively), the tissue may be joint tissue, such as intervertebral disc tissue, or non-joint tissue, such as bone of bone. [0003] In the case of intervertebral discs, the field of interest of the present invention is to restore the function of a patient's spine with minimally invasive spinal surgery, especially for patients whose discs are in the process of dwindling due to degeneration of the nucleus pulposus. Background technique [0004] In tissue engineering, the problem is seen in regenerating tissues with a biological structure and specific biomechanical properties, especially joint tissues, such as intervertebral disc tissue, and non-joint tissues, such as bone tissue of bones. [0005] Joint tissue is present in the spine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52C08L5/04A61L27/28A61L27/22
CPCA61L2430/38A61L2400/06A61L27/52A61L27/26C08L5/08C08L5/04
Inventor 朱塞佩·卡尔沃萨农齐亚·贝尔纳迪尼路易吉·拉泽里玛丽亚·格拉西亚·卡斯克内
Owner 朱塞佩·卡尔沃萨
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