Gram bacteria retest real-time fluorescent quantitative PCR detection reagent kit and use

Abstract

The invention provides a gram double error detection real-time fluorescence quantitative PCR detection kit which consists of quantitative PCR reaction liquid (1), a gram positive standard (2), a gram negative standard (3), a positive reference substance (4), a negative reference substance (5), bacteria DNA extraction lysis liquid (6) and a box body (8). The box body (8) is equipped with vessel holes for respectively arranging a PCR reaction liquid tube, the standards, the reference substances and the bacteria DNA extraction lysis liquid. The kit of the invention is reasonably designed. The real-time fluorescence quantitative PCR technology is used. Bacteria universal primers and gram negative and positive double-specific fluorescence probes are adopted. Through the real-time fluorescence quantitative, both the typing of gram negative and positive bacteria and the real-time accurate quantitative on the detected negative and positive bacteria are implemented. The invention can be applied to the detection of bacteria gram negative and positive DNA.

Description

Real-time fluorescent quantitative PCR detection kit and application of bacterial Gram double detection technical field The invention belongs to the field of biological technology, and relates to a quantitative PCR detection kit, in particular to a dual-probe real-time fluorescent quantitative PCR for detecting bacterial Gram-negative and positive bacterial types in blood, cerebrospinal fluid, pleural effusion, ascites and other samples of children with bacterial infection and real-time quantitative methods. Background technique Sepsis refers to a disease in which bacteria enter the blood circulation, grow and reproduce in it, and produce toxins, causing severe systemic infection. Children, especially newborns, are susceptible to sepsis due to poor self-resistance, thin and tender skin, and unhealed umbilicus after birth. If children's sepsis cannot be diagnosed early and treated timely and thoroughly, it may cause purulent meningitis, affect children's intellectual devel...

AI Technical Summary

Problems solved by technology

However, due to the time-consuming, low positive rate, easy contamination, and non-specificity of some indicators, it is difficult to make a rapid and accurate judgment on sepsis in children.

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