Method for acquirement immunological tolerance birds

Abstract

The invention provides a method for obtaining immunologic tolerance poultry. The method is: heterologous antigen is introduced into the poultry embryo in early poultry embryo development, and poultry immune system is induced to have immune tolerance with the injected heterologous protein. The heterologous antigen is injected into chicken embryo by using the invention, and chicken immune system can be successfully induced to have immune tolerance with the injected heterologous protein in embryo period, and furthermore the immunologic tolerance chicks are gained. The tolerance rate of chicks can be achieved 53.8 percent, and the effect of immunologic tolerance can be kept more than two months, and the hatching rate can be achieved 65 percent.

Description

technical field [0001] The invention belongs to the field of immunobiology, and in particular relates to an embryo blood vessel microinjection method for obtaining immune tolerance poultry. Background technique [0002] Immunological tolerance can be defined as a physiological state in which the immune system does not engage in destructive immune responses to innate components of the body or to introduced antigens, which can be artificially induced by introducing antigens into the developing immune system. However, for most transgenic animals, especially those used as bioreactors, foreign genes are usually expressed in adult animals with fully developed immune systems. In itself, it is a "non-self" substance, so it is likely to trigger a specific immune response. [0003] There may be two results of this immune response: one is that the immune system functions to eliminate the foreign substances that appear in the body; the other is that the stimulation of these foreign sub...

AI Technical Summary

Problems solved by technology

[0006] One is that the proportion of induced immune tolerance chickens in the experimental group is not high, and the time to maintain immune tolerance is short, because the foreign protein antigen is injected in the yolk and does not directly contact the immune system, or their contact Need to rely on the absorption process of yolk nutrients, which reduces the probability of obtaining immune tolerant chicks

The second is that the cost of the experiment is too high, because the exogenous protein antigen is injected into the egg yolk, so after dilution of the egg yolk, a very high dose is required to induce the generation of immune tolerance in chickens, and the commercialized purified protein is expensive and requires spend a lot of money to buy

And this method is less likely to obtain immune immune tolerance chicks, so a large number of samples (egg numbers) are needed to maintain the needs of research and production applications, and the purchase of breeding eggs, incubation costs can not be ignored, which greatly increases the experimental cost. the cost of

[0007] Another way to obtain immune-tolerant chickens is to immunize freshly hatched chicks with exogenous protein antigens, but at this time the immune system of the chicks has been fully developed, so the probability of obtaining immune-tolerant chickens is even smaller. not adopted

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