Method for testing peroxidase content in cow's milk

Abstract

The invention provides a method for testing components in milk, especially a method for testing peroxidase content in milk. The invention belongs to the field of testing technology. The method for testing peroxidase content in milk by a capillary electrophoresis apparatus provided by the invention includes the steps of pretreatment, sample test, and also includes the steps of calibrating readings, editing reports and so on. The invention includes the following steps: doing electrophoresis by the capillary electrophoresis apparatus, detecting the absorption value of the sample on the capillary chromatograph system, expressing the value in map form, then the peroxidase content in milk is detected accurately, in order to provide technical supports for product development, quality control and component analysis.

Description

technical field

[0001] The invention relates to a method for analyzing and detecting protein in milk, in particular to a method for detecting the content of lactoperoxidase in milk. It belongs to the technical field of detection. Background technique

[0002] At present, there is no national standard or industry standard for the detection method of lactoperoxidase content in China. Relevant domestic standards include obsolete standards and GB / T15550-1995 Code of Practice for Preserving Raw Milk with Lactoperoxidase System in Activated Milk. Abroad, relevant standards include DIN-10483-1-2002 Determination of milk lactoperoxidase activity. Part 1: Photometric method (reference method), DIN-10483-2-2002 Determination of milk lactoperoxidase activity. Part 2: Reflectometer method. The commonly used detection method for lactoperoxidase is the microplate reader method. Although the steps of this method are simple, it takes a long time to detect the result, about 2 hours. Thi...

Examples

Embodiment 1

[0027] Embodiment 1 A kind of detection method of lactoperoxidase content in milk

[0028] The capillary electrophoresis temperature was controlled at 40°C, a quartz capillary column with a diameter of 50 μm and a length of 20 mm was used, a DAD detector was used, the injection pressure was 0.5 psi, the injection time was 10 s, and the working voltage was 25 kV.

[0029] Formulation of the calibration curve: Standard solutions with concentrations of 2 mg / ml, 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, and 0.1 mg / ml were prepared using lactoperoxidase standard products. After shaking well, use high-pressure capillary electrophoresis analysis. The peak heights after integration are 5782, 2741, 1451, 634, and 220 respectively. The standard curve is obtained by using software. The linear regression correlation coefficient is 0.9992, the lowest detection line is 0.1ug, and the lowest detection line The output content is 0.001mg / ml, and the standard sample recovery rate reaches 91%.

[0030] P...

Embodiment 2

[0034] Embodiment 2 The detection method of lactoperoxidase content in a kind of milk

[0035] The capillary electrophoresis temperature was controlled at 25°C, a quartz capillary column with a diameter of 75 μm and a length of 600 mm was used, a DAD detector was used, the injection pressure was 0.5 psi, the injection time was 5 s, and the working voltage was 25 kV.

[0036]Formulation of calibration curve: Standard solutions with concentrations of 2mg / ml, 1mg / ml, 0.5mg / ml, 0.25mg / ml, and 0.1mg / ml were prepared using lactoperoxidase standard products. After shaking well, use high-pressure capillary electrophoresis to analyze. The peak heights after integration are 5982, 2790, 1370, 584, and 268 respectively. The standard curve is obtained by using software. The linear regression correlation coefficient is 0.998, the lowest detection line is 0.2ug, and the lowest detection line The output content is 0.001mg / ml, and the standard sample recovery rate reaches 90%.

[0037] Proces...

technical field

[0001] The invention relates to a method for analyzing and detecting protein in milk, in particular to a method for detecting the content of lactoperoxidase in milk. It belongs to the technical field of detection. Background technique

[0002] At present, there is no national standard or industry standard for the detection method of lactoperoxidase content in China. Relevant domestic standards include obsolete standards and GB / T15550-1995 Code of Practice for Preserving Raw Milk with Lactoperoxidase System in Activated Milk. Abroad, relevant standards include DIN-10483-1-2002 Determination of milk lactoperoxidase activity. Part 1: Photometric method (reference method), DIN-10483-2-2002 Determination of milk lactoperoxidase activity. Part 2: Reflectometer method. The commonly used detection method for lactoperoxidase is the microplate reader method. Although the steps of this method are simple, it takes a long time to detect the result, about 2 hours. Thi...

Examples

Embodiment 1

[0027] Embodiment 1 A kind of detection method of lactoperoxidase content in milk

[0028] The capillary electrophoresis temperature was controlled at 40°C, a quartz capillary column with a diameter of 50 μm and a length of 20 mm was used, a DAD detector was used, the injection pressure was 0.5 psi, the injection time was 10 s, and the working voltage was 25 kV.

[0029] Formulation of the calibration curve: Standard solutions with concentrations of 2 mg / ml, 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, and 0.1 mg / ml were prepared using lactoperoxidase standard products. After shaking well, use high-pressure capillary electrophoresis analysis. The peak heights after integration are 5782, 2741, 1451, 634, and 220 respectively. The standard curve is obtained by using software. The linear regression correlation coefficient is 0.9992, the lowest detection line is 0.1ug, and the lowest detection line The output content is 0.001mg / ml, and the standard sample recovery rate reaches 91%.

[0030] P...

Embodiment 2

[0034] Embodiment 2 The detection method of lactoperoxidase content in a kind of milk

[0035] The capillary electrophoresis temperature was controlled at 25°C, a quartz capillary column with a diameter of 75 μm and a length of 600 mm was used, a DAD detector was used, the injection pressure was 0.5 psi, the injection time was 5 s, and the working voltage was 25 kV.

[0036]Formulation of calibration curve: Standard solutions with concentrations of 2mg / ml, 1mg / ml, 0.5mg / ml, 0.25mg / ml, and 0.1mg / ml were prepared using lactoperoxidase standard products. After shaking well, use high-pressure capillary electrophoresis to analyze. The peak heights after integration are 5982, 2790, 1370, 584, and 268 respectively. The standard curve is obtained by using software. The linear regression correlation coefficient is 0.998, the lowest detection line is 0.2ug, and the lowest detection line The output content is 0.001mg / ml, and the standard sample recovery rate reaches 90%.

[0037] Proces...