Detection method of trimethylsulfonium in tea by liquid chromatography tandem mass spectrometry
A liquid chromatography, trimethyl sulfonium technology, applied in the detection field of trimethyl sulfonium
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[0018] The present invention relates to each instrument and medicine specification as follows:
[0019] Thermo TSQ Quantan Access liquid chromatography-tandem mass spectrometer: with electrospray ion source (ESI) and automatic sampler (Finnigen Surveyor); electronic balance: sensitivity 0.1mg (Mettler, Switzerland); 3K15 high-speed refrigerated centrifuge machine (Sigma);
[0020] Acetonitrile is chromatographically pure (Merck, Germany); formic acid, ammonium acetate, and hydrogen peroxide are analytically pure (Shanghai Sinopharm); N-propylethylenediamine solid powder (PSA, Shanghai Anpu Company). The experimental water was Milli-Q high-purity water. Tall beaker (diameter 30mm, 50mL). 15mL graduated centrifuge tube (Shanghai Anpu Company). Graphitized carbon black (GCB, Shenzhen Comma Company). Hydrophilic membrane (0.22um, Shanghai Anpu Company). Sample extract (0.77g ammonium acetate / 1000mL water, containing 2mL formic acid).
[0021] The standard product of trimethy...
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