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Method for measuring free water content and bound water content of animal tissues

A technology of animal tissue and bound water, which is applied in the medical field and can solve the problems of scarce research on free water and bound water

Inactive Publication Date: 2018-05-01
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on free water and bound water in animal tissues is extremely rare, and the method for its content determination has never been reported.

Method used

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  • Method for measuring free water content and bound water content of animal tissues
  • Method for measuring free water content and bound water content of animal tissues
  • Method for measuring free water content and bound water content of animal tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: In this experiment, the normal muscle tissue of mice under physiological state and the liver cancer tissue of mice under pathological state were obtained, and the content of free water and bound water in cells was detected by heating weighing method, and the heating conditions and weighing time were explored at the same time. In order to establish a method for the determination of free water and bound water content in animal tissues.

[0021] 1 experiment

[0022] 1.1 Animals and tumor strains

[0023] Animals: SPF male KM mice, weighing 20 ± 2 g, provided by the Experimental Animal Center of Jiangxi University of Traditional Chinese Medicine, license number: SCXK (Gan) 2011-0001.

[0024] Tumor strain: mouse H22 hepatoma cells Source: provided by Tumor Cell Bank of Chinese Academy of Medical Sciences.

[0025] 1.2 Instruments

[0026] Inverted microscope (German Leica, DMI3000B); electric heating blast drying oven (Shanghai Boxun Industrial Co., Ltd. Medi...

Embodiment 2

[0040] Example 2: In this experiment, the normal muscle tissue of mice under physiological state and the liver cancer tissue of mice under pathological state were obtained, and the content of free water and bound water in cells was detected by heating weighing method, and the heating conditions and weighing time were explored at the same time. In order to establish a method for the determination of free water and bound water content in animal tissues.

[0041] 1 experiment

[0042] 1.1 Animals and tumor strains

[0043] Animals: SPF male KM mice, weighing 20 ± 2 g, provided by the Experimental Animal Center of Jiangxi University of Traditional Chinese Medicine, license number: SCXK (Gan) 2011-0001.

[0044] Tumor strain: mouse H22 hepatoma cells Source: provided by Tumor Cell Bank of Chinese Academy of Medical Sciences.

[0045] 1.2 Instruments

[0046] Inverted microscope (German Leica, DMI3000B); electric heating blast drying oven (Shanghai Boxun Industrial Co., Ltd. Medi...

Embodiment 3

[0060] Example 3: In this experiment, the normal muscle tissue of mice under physiological state and the liver cancer tissue of mice under pathological state were obtained, and the content of free water and bound water in cells was detected by heating weighing method, and the heating conditions and weighing time were explored at the same time. In order to establish a method for the determination of free water and bound water content in animal tissues.

[0061] 1 experiment

[0062] 1.1 Animals and tumor strains

[0063] Animals: SPF male KM mice, weighing 20 ± 2 g, provided by the Experimental Animal Center of Jiangxi University of Traditional Chinese Medicine, license number: SCXK (Gan) 2011-0001.

[0064] Tumor strain: mouse H22 hepatoma cells Source: provided by Tumor Cell Bank of Chinese Academy of Medical Sciences.

[0065] 1.2 Instruments

[0066] Inverted microscope (German Leica, DMI3000B); electric heating blast drying oven (Shanghai Boxun Industrial Co., Ltd. Medi...

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Abstract

The invention discloses a method for measuring free water content and bound water content of animal tissues. The method comprises the following steps: preparing animals, tumor strains and instruments;dividing the animals into a physiological group and a pathological group; injecting H22 cells into the armpits of the animals in the pathological group and establishing an H22 tumor-bearing model; molding the H22 tumor-bearing model for 14 days and taking out the right back limb tissue; heating; performing data processing; performing statistical analysis. The method has the following advantages:the free water content and the bound water content of the animal tissues can be effectively detected, different tissues need to be baked for different time to achieve constant weight, through heatingand weighing, the free water in the physiological group must be heated at 75 DEG C for 4 hours and the free water in the pathological group must be heated at 75 DEG C for 5 hours; weak bound water ofthe physiological group and the pathological group must be heated at 100 DEG C for 2 hours, strong bound water of the physiological group and the pathological group must be heated at 120 DEG C for 1 hour, and research on the free water and bound water in the animal tissues is beneficial to early diagnosis of tumor diseases and research on anti-tumor medicines and can be applied to research on anti-ageing medicines.

Description

technical field [0001] The invention relates to a method for measuring the content of free water and bound water in animal tissues, and belongs to the technical field of medicine. Background technique [0002] The research of free water and bound water is mainly used in the fields of construction, water conservancy, chemical industry and agriculture. However, the research on free water and bound water of animal tissues is extremely rare, and the method for the determination of its content has never been reported. [0003] There are two forms of water in animal tissues: free water and bound water. As a good solvent, free water can transport nutrients in organisms or cells to various cells, and transport metabolic wastes to excretory organs. The bound water is weakly bound water and strongly bound water, which is bound to organic solid substances by hydrogen bonds and polar bonds. The determination of the content of free water and bound water in cells is of great significan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N5/04
CPCG01N5/045
Inventor 谢斌余功张晓勇陈江涛胡桥
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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