31results about How to "Effective detection content" patented technology

The invention discloses a fritillaria medicinal material identification and content determination method comprising the steps: after extracting with ethanol, passing through a neutral alumina column, determining by high performance liquid chromatography, combining with specific chromatograms of medicinal materials, effectively identifying thunberg fritillary bulb, bulbus fritillariae cirrhosae and bulbus fritillariae ussuriensis, and determining the contents of peimine and peiminine. The method not only can effectively identify authenticity of the three fritillaria medicinal materials, but also can accurately determine for the contents of peimine and peiminine, achieves multiple evaluations through one-time determination, and is safe, environmentally friendly, convenient and quick.

The invention discloses a method for determining content and purity of L-carnitine in milk powder, comprising the following steps in sequence: 1) taking the milk powder as a sample for sample treatment; 2) preparing standard solutions respectively by taking an L-carnitine standard substance and a D-carnitine standard substance; 3) correspondingly preparing an L-carnitine standard solution and a D-carnitine standard solution obtained the step 2) into an L-carnitine derivative solution containing gradient concentration and a D-carnitine solution containing gradient concentration; 4) carrying out derivatization reaction on the sample solutions; 5) carrying out high performance liquid chromatography on the products obtained in the step 3) and step 4); 6) acquiring the concentration cL of the L-carnitine and the concentration cD of the D-carnitine; and 7) acquiring the content and the purity of the L-carnitine in the milk powder sample. By adopting the method for determining the content and the purity of L-carnitine in the milk powder of the invention, the detection sensitivity can be greatly improved.

The invention provides a method for testing components in milk, especially a method for testing peroxidase content in milk. The invention belongs to the field of testing technology. The method for testing peroxidase content in milk by a capillary electrophoresis apparatus provided by the invention includes the steps of pretreatment, sample test, and also includes the steps of calibrating readings, editing reports and so on. The invention includes the following steps: doing electrophoresis by the capillary electrophoresis apparatus, detecting the absorption value of the sample on the capillary chromatograph system, expressing the value in map form, then the peroxidase content in milk is detected accurately, in order to provide technical supports for product development, quality control and component analysis.

The invention discloses a method for detecting 2,2,6,6-tetramethylpiperidinooxy with high performance liquid chromatography-mass spectrometry. The method comprises the steps that 1, a test solution and a reference stock solution is prepared; 2, the test solution and the reference stock solution with a certain concentration gradient are subjected to sample introduction, a high performance liquid chromatograph mass spectrometer is used for detecting and recording a chromatogram; and 3, linear regression analysis is performed on the mass concentration of the reference stock solution and chromatogram peak area, a regression equation and correlation coefficients are obtained, and a standard curve is manufactured; and the peak area of 2,2,6,6-tetramethylpiperidinooxy in the chromatogram of the test solution is utilized to calculate the content of 2,2,6,6-tetramethylpiperidinooxy by an external standard method. The method provided by the invention is the first method for detecting 2,2,6,6-tetramethylpiperidinooxy developed in the field. The method has accurate detection result, high sensitivity, good stability, and low detection limit, and totally meets detection requirements for genotoxic impurities in the field.

The invention relates to a method for detecting components in milk, in particular to a method for detecting the content of Beta-lactoglobulin, belonging to the detection technical field; the detection method using a capillary electrophoresis meter to detect the content of Beta-lactoglobulin of the invention comprises the following steps: the pretreatment of samples, detection of sample-loading, alignment of reading and edit of report, etc.; the invention is that the samples are processed by electrophoresis on the capillary electrophoresis meter, thus detecting absorbance of the samples by a capillary chromatographic system, expressing the absorbance by the mode of mapping, detecting the content of Beta-lactoglobulin accurately and providing the technical support for the development of products, quality control and analysis of the components.

The invention relates to a method for detecting components in milk, in particular to a detection method for detecting the content of milk immunoglobulin (IgG), and belongs to the detection technology field. The detection method for detecting the content of the milk immunoglobulin (IgG) through a capillary electrophoresis instrument of the invention comprises the steps of sample pretreatment and sample loading detection, reading calibration, and report editing, etc. In the invention, the electrophoresis of a sample runs on the capillary electrophoresis instrument, and the absorption value of the sample is detected by a capillary chromatographic system and expressed in the form of mapping, thereby the content of the milk immunoglobulin (IgG) can be accurately detected, so as to provide technology support for product development, quality control and component analysis.

The invention discloses a stable and sensitive type IV collagen (IV-C) detection reagent. The stable and sensitive type IV collagen (IV-C) detection reagent comprises a reagent R1 and a reagent R2; areaction system is optimized, TAPSO (N-[Tris(hydroxymethyl)metyl]-3-amino-2-hydroxypropanesulfonic acid) buffer is adopted, a plurality of stabilizing agents including polyvinylpyrrolidone, gamma-cyclodextrin, xanthan gum, NaCl, trehalose, PEG 6000, and glycerol are added, so that reagent stability is improved obviously; goat anti-human type IV collagen antibody coated latex particles are adopted,in detection, mutual crosslinking of adjacent latex particles is realized through antigen-antibody specific immunization reaction in detection, so that reagent sensitivity and accuracy are increasedobviously; and in addition, combination of novel nonionic surfactants RHEODOL, TW-O320V (tween 85) and AMINON C-11S (methyl cocoate alcohol amide) is adopted, so that antibody stability is promoted and maintained, system turbidity is avoided, and reagent stability is improved further.

The invention relates to an ultra-high performance liquid chromatography analysis method for detecting mannose in honey. The method comprises the following steps: performing linear equation and standard chromatogram, performing sample pretreatment, and performing ultra-high performance liquid chromatography condition detection on a pretreated sample. The method can effectively detect the content of mannose in the honey, has good sensitivity, and can identify whether the honey is adulterated according to the content of mannose in the honey.

The invention provides a device for noninvasively detecting indocyanine green content in bodies. The device includes a laser emitting device that can emit single wavelength or multi-wavelength with the wavelength of 805 nm, and photoacoustic signal detection and conversion devices; and preferably, the device also includes an ultrasonic positioning device, and the ultrasonic positioning device canaccurately detect the indocyanine green content in a blood vessel at a specific position without being disturbed by other tissue. Through the device, the noninvasive detection on the indocyanine greencontent in blood can be realized; rapid assessment on hepatic functional reserve can be realized; and therefore, the device has good commercial prospects.

The invention belongs to the field of drug detection, and particularly relates to a method for determining photodegradation impurities in a levofloxacin raw material and a levofloxacin preparation. The method comprises the following steps: (1) preparing a levofloxacin photodegradation impurity standard solution; (2) injecting the standard solution into a high performance liquid chromatograph for determination, and constructing a standard curve by taking the concentration of the standard solution as an abscissa and the peak area as an ordinate; (3) preparing a to-be-detected sample solution; and (4) injecting the to-be-detected sample solution into a high performance liquid chromatograph for determination, recording the peak area of the to-be-detected sample solution, and calculating the content of the photodegradable impurities by using the standard curve. According to the method, high performance liquid chromatography is adopted, amino silane bonded silica gel is used as a stationaryphase, a mixed solution of acetonitrile and 0.05mol/L monopotassium phosphate solution is used as a mobile phase, and an isocratic elution mode is adopted. The method is high in specificity, high in precision, good in accuracy and capable of effectively detecting the content of photodegradation impurities in levofloxacin raw materials and preparations.

The invention discloses a detection method of detecting trimethylsulfonium in tea leaves by a liquid chromatograph-tandem mass spectrometer. The detection method comprises the following steps of 1) sample pretreatment; and 2) chromatogram and mass spectrum conditions of detection with liquid chromatography-tandem mass spectrometry: a mobile phase: organic phase is acetonitrile, a water phase is anammonium acetate-formic acid solution which has the concentration of 15mmol/L and contains 1.5mL of formic acid per L; gradient elution: a flow rate is 0.30mL/min, and a feeding amount is 25 microlitres; ion source: electrospray ionization (ESI) with positive ion scanning; a mass spectrum scanning manner is multiple reaction monitoring (MRM); spray voltage is 4,200V; shell gas pressure is 40arb;and collision gas (Ar/m) Torr: 1.5. By adopting the detection method, the recovery rate and the precision accord with relevant technical specifications, residues of the trimethylsulfonium in the tea leaves can be effectively detected, and the detection requirements for a residue limit of the trimethylsulfonium in the tea leaves of the European Union are met.

The invention relates to a method for detecting human milk ingredient, in particular to a method for detecting immune globulin IgM content in human milk, comprising: (1) dissolving a human immune globulin IgM standard substance into sample buffer solution to prepare to obtain multiple standard solutions with different contents of the standard substance, using a high voltage capillary electrophoresis analyser to respectively detect and analyze the standard solution to obtain a detection result, and drawing a standard curve according to the concentration of the standard solution and the corresponding detection result; (2) after a human milk sample is centrifuged, utilizing the high voltage capillary electrophoresis analyser to detect the sample; (3) comparing the detection result of the sample with the standard curve to obtain the human immune globulin IgM content in human milk sample. The invention has the characteristics of accurate detection result, high repeatability and low cost.

The invention relates to a method for detecting the immunoglobulin IgA content in human milk, in particular to a detection method for detecting immunoglobulin IgA content in human milk. The method comprises the following steps: (1) dissolving human immunoglobulin IgA standards in sample buffer solutions, preparing multiple standard solutions with different standard contents, using a high-pressure capillary electrophoresis apparatus to respectively detect and analyze the standard solutions to obtain detection results, and drawing standard curves based on the concentrations of the standard solutions and the corresponding detection results; (2) centrifuging a human milk sample, and then detecting the sample by using the high-pressure capillary electrophoresis apparatus; and (3) comparing the detection result of the sample with the standard curves to obtain the human immunoglobulin IgA content in the human milk sample. The invention has the characteristics of accurate detection result, high repeatability and low cost.