Method for detecting content of beta-lactoglobulin in cow's milk

A lactoglobulin and detection method technology, applied in the field of analysis and detection of protein in milk, can solve the problem that there is no national standard and industry standard for the detection method of β-lactoglobulin content.

Active Publication Date: 2008-10-29
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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AI Technical Summary

Problems solved by technology

[0003] At present, there is no national standard or industry standard for the detection method of β-lactoglobulin content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 A kind of detection method of β-lactoglobulin content in cow's milk

[0028] The capillary electrophoresis temperature was controlled at 25°C, a quartz capillary column with a diameter of 50 μm and a length of 20 mm was used, a DAD detector was used, the injection pressure was 0.5 psi, the injection time was 10 s, and the working voltage was 15 kV.

[0029] Formulation of calibration curve: Standard solutions with concentrations of 2mg / ml, 1mg / ml, 0.5mg / ml, 0.25mg / ml, and 0.1mg / ml were prepared using β-lactoglobulin standard substances. After shaking well, use high-pressure capillary electrophoresis analysis. The peak areas after integration are 378089, 152088, 72110, 33124, and 14112 respectively. The standard curve is obtained by using software. The linear regression correlation coefficient is 0.9917, the lowest detection line is 0.01ug, and the lowest detection line The output content is 0.01mg / ml, and the standard sample recovery rate reaches 90%.

[0...

Embodiment 2

[0034] Embodiment 2 The detection method of β-lactoglobulin content in a kind of milk

[0035]The capillary electrophoresis temperature was controlled at 30°C, a quartz capillary column with a diameter of 75 μm and a length of 600 mm was used, a DAD detector was used, the injection pressure was 0.5 psi, the injection time was 5 s, and the working voltage was 25 kV.

[0036] Formulation of calibration curve: Standard solutions with concentrations of 2mg / ml, 1mg / ml, 0.5mg / ml, 0.25mg / ml, and 0.1mg / ml were prepared using β-lactoglobulin standard substances. After shaking well, use high-pressure capillary electrophoresis to analyze. The peak areas after integration are 393142, 178563, 87892, 43728, and 21157. The standard curve is obtained by using software. The linear regression correlation coefficient is 0.998, the lowest detection line is 0.01ug, and the lowest The detected content is 0.01 mg / ml, and the recovery rate of the standard sample reaches 95%.

[0037] Processing samp...

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Abstract

The invention relates to a method for detecting components in milk, in particular to a method for detecting the content of Beta-lactoglobulin, belonging to the detection technical field; the detection method using a capillary electrophoresis meter to detect the content of Beta-lactoglobulin of the invention comprises the following steps: the pretreatment of samples, detection of sample-loading, alignment of reading and edit of report, etc.; the invention is that the samples are processed by electrophoresis on the capillary electrophoresis meter, thus detecting absorbance of the samples by a capillary chromatographic system, expressing the absorbance by the mode of mapping, detecting the content of Beta-lactoglobulin accurately and providing the technical support for the development of products, quality control and analysis of the components.

Description

technical field [0001] The invention relates to a method for analyzing and detecting protein in milk, in particular to a method for detecting β-lactoglobulin content in milk. It belongs to the technical field of detection. Background technique [0002] β-lactoglobulin is an important bovine whey protein, accounting for 8% to 10% of the total protein mass fraction, 20-40mg / ml. The molecular weight of β-lactoglobulin is about 18400D, mainly composed of 162 amino acids. The secondary structure of β-lactoglobulin is composed of 9 antiparallel β-sheets and 1 helical molecule. β-lactoglobulin is the main allergen of milk allergy in infants. This is because β-lactoglobulin is adsorbed on the intestinal mucosa, causing an immune response and causing allergies. Therefore, reducing the content of β-lactoglobulin can help improve infant allergies. Therefore, it is of great significance to accurately detect the content of β-lactoglobulin. [0003] At present, there is no national ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N1/38G01N21/25
Inventor 孙国庆康小红胡新宇刘卫星
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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