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Method for determining content and purity of L-carnitine in milk powder

A determination method and carnitine technology are applied in the field of determination of L-carnitine content and purity in milk powder, can solve the problem of inability to distinguish carnitine enantiomers and the like, and achieve the effects of good accuracy, high sensitivity and good separation ability

Inactive Publication Date: 2011-02-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In terms of the detection of L-carnitine in milk powder, the analytical method proposed in Patent No. 02100582.6 "A Method for Determination of L-Carnitine Content in Milk Powder by Enzyme-Colorimetry" cannot distinguish the enantiomers of carnitine
[0005] Determination of L-carnitine in infant milk powder by pre-column derivatization high performance liquid chromatography has not been reported yet

Method used

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  • Method for determining content and purity of L-carnitine in milk powder
  • Method for determining content and purity of L-carnitine in milk powder
  • Method for determining content and purity of L-carnitine in milk powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, a kind of assay method of L-carnitine content and purity in milk powder is characterized in that comprising the following steps successively:

[0054] 1), sample processing:

[0055] Accurately weigh 16.65g of a commercially available common milk powder sample, dissolve it in 30ml of deionized water, bathe in 50°C water for 15min, and ultrasonic (frequency 30kHz) for 10min to fully dissolve the milk powder, then add deionized water to make up to 100ml.

[0056] Then adjust the pH to 2 with 0.1mol / L hydrochloric acid solution, let it stand for 10 minutes, and then filter it with natural filter paper. The pH of the obtained filtrate was adjusted to 6 with 1mol / L NaOH solution, and after standing for 10 minutes, a second filtration was performed with natural filter paper, and the filtrate obtained from the second filtration was the sample solution.

[0057] 2), prepare standard solution:

[0058] Weigh 50 mg of L-carnitine standard substance, dissolve it in...

Embodiment 2

[0088] Embodiment 2, a kind of assay method of L-carnitine content and purity in milk powder is characterized in that comprising the following steps successively:

[0089] 1), sample processing:

[0090] Accurately weigh 14.95g of commercially available infant formula milk powder No. 1 sample, dissolve it in 30m deionized water, bathe in 50°C water for 15min, ultrasonic (frequency 30kHz) 10min, the milk powder is fully dissolved, add deionized water to make up to 100ml.

[0091] Then adjust the pH to 1.9 with 0.1mol / L hydrochloric acid solution, let it stand for 10min, and then filter it with natural filter paper. Adjust the pH of the obtained filtrate to 6.1 with 1 mol / L NaOH solution, and perform a second filtration with natural filter paper, and the filtrate obtained from the second filtration is the sample solution.

[0092] Step 2) to step 6) are all the same as in Example 1.

[0093] Thereby obtain respectively the concentration cL=(812098*2*10 of L-carnitine in the sa...

Embodiment 3

[0097] Example 3. The commercially available infant formula milk powder No. I sample in Example 2 was changed to the commercially available infant formula milk powder No. II sample, and 15.63 g of the sample was accurately weighed.

[0098] Step 1) to step 6) are all the same as in Example 2.

[0099] Thereby get respectively the concentration cL=(848302*2*10 of L-carnitine in the sample solution -7 +0.0978)*10*1000 / 60=44.57 μg / ml, the concentration of D-carnitine cD=0 μg / ml;

[0100] 7), obtain the content and purity of L-carnitine in the sample:

[0101] The calculation formula is the same as in Example 1.

[0102] The results were as follows: the content of L-carnitine in No. II sample of infant formula milk powder was 28.52mg / 100g, and D-carnitine was not detected.

[0103] In order to prove the correctness of the conclusion of the present invention, utilize the existing high-performance liquid chromatography ultraviolet analysis method to completely the common milk pow...

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Abstract

The invention discloses a method for determining content and purity of L-carnitine in milk powder, comprising the following steps in sequence: 1) taking the milk powder as a sample for sample treatment; 2) preparing standard solutions respectively by taking an L-carnitine standard substance and a D-carnitine standard substance; 3) correspondingly preparing an L-carnitine standard solution and a D-carnitine standard solution obtained the step 2) into an L-carnitine derivative solution containing gradient concentration and a D-carnitine solution containing gradient concentration; 4) carrying out derivatization reaction on the sample solutions; 5) carrying out high performance liquid chromatography on the products obtained in the step 3) and step 4); 6) acquiring the concentration cL of the L-carnitine and the concentration cD of the D-carnitine; and 7) acquiring the content and the purity of the L-carnitine in the milk powder sample. By adopting the method for determining the content and the purity of L-carnitine in the milk powder of the invention, the detection sensitivity can be greatly improved.

Description

technical field [0001] The invention relates to a method for determining the content and purity of L-carnitine in milk powder; in particular, it relates to a method for determining the content and purity of L-carnitine in milk powder by pre-column derivatization high performance liquid chromatography. Background technique [0002] The synthesis ability of L-carnitine in infants is weak, so it must be ingested from outside sources. Therefore many countries and regions in the world have added L-carnitine in baby milk powder, and domestic also has the baby milk powder that adds L-carnitine to go on the market. And carnitine has two forms of existence (L-type and D-type) in nature, and D-carnitine has negative effects on human health, and chemically synthesized L-carnitine is generally a mixture of L-carnitine and D-carnitine, and then separates and removes D-carnitine. produced by carnitine. [0003] The detection of L-carnitine in domestic health care products generally adop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 朱加进童玲冯盘朱方
Owner ZHEJIANG UNIV
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