Enzyme-linked immune sorbent assay (ELISA) kit for detecting Shiga toxin II (StxII)

A technology in the kit and sequence listing is applied in the field of preparation of monoclonal antibodies S2D8 and S2C6, which can solve the problems of delaying the best time for treatment and being difficult to distinguish, and achieves a high-accuracy and easy-to-use method to curb the spread of the epidemic. Effect
CN103235140BActive Publication Date: 2015-05-27JIANGSU BIG DIPPER ENVIRONMENTAL PROTECTION CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGSU BIG DIPPER ENVIRONMENTAL PROTECTION CO LTD
Publication Date
2015-05-27

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Abstract

The invention discloses an enzyme-linked immune sorbent assay (ELISA) kit for detecting Shiga toxin II (StxII) and also discloses sequence information of monoclonal antibodies S2D8 and S2C6 in the ELISA kit and a preparation process of the monoclonal antibodies S2D8 and S2C6. The ELISA kit comprises the monoclonal antibody S2D8, the HRP-marked monoclonal antibody S2C6, a horse radish peroxidase substrate buffer solution, a protein standard product StxII (100 ug / ml, 0.1 ml), a negative control sample BSA and lotion (PBS+0.05%Tween20). The kit can sensitively detect the StxII content of samples, and the sensitivity can reach 4 ng / ml. The kit provided by the invention also can effectively identify subtype StxIIc and StxIIvha of the StxII.
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Description

technical field

[0001] The invention relates to an ELISA kit, in particular to an ELISA kit for detecting type II Shiga toxin (StxII), and also relates to the preparation of monoclonal antibodies S2D8 and S2C6 in the ELISA kit and the method for using the kit. Background technique

[0002] Enterohemorrhagic E.coli (EHEC) infection is an important zoonotic disease, which is prevalent all over the world, including developed and developing countries, and its infection is fulminant, highly pathogenic and fatal It has become a global public health problem and poses a huge threat to human health. EHEC infection can cause diarrhea (Diarrhea), hemorrhagic colitis (Hemorrhagic Colitis, HC), can also cause hemolytic uremic syndrome (Hemolytic Uremic Syndrome, HUS), thrombotic thrombocytopenic purpura (Thromboric Thrombocytopenic Porpura, TTP) ) and other serious complications, especially HUS can cause irreversible functional damage to the kidneys of patients, and the mortality rate i...

Claims

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