Method for detecting immunoglobulinlg content in cow's milk

A technology of immunoglobulin and detection method, which is applied in the field of detection of immunoglobulin content in milk, and can solve problems such as undeveloped detection of milk immunoglobulin

Inactive Publication Date: 2008-12-03
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, capillary electrophoresis has not yet b

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1 A method for detecting the content of immunoglobulin (IgG) in milk

[0028] The capillary electrophoresis temperature is controlled at 25°C, using a quartz capillary column with a diameter of 50μm and a length of 20mm, a DAD detector, the injection pressure is 0.5psi, the injection time is 10s, and the working voltage is 15kV.

[0029] Formulation of the calibration curve: use milk immunoglobulin (IgG) standard to prepare standard solutions with concentrations of 2 mg / ml, 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, and 0.1 mg / ml. After shaking, use high-pressure capillary electrophoresis analysis. The integrated peak areas are 189614, 87015, 44216, 20897, 13210. The standard curve is obtained by software. The linear regression correlation coefficient is 0.9973, the lowest detection line is 0.009ug, and the lowest detection The output content is 0.01mg / ml, and the recovery rate of standard samples is 90%.

[0030] Processing sample buffer configuration method: add 40m mol / L 3-...

Example Embodiment

[0034] Example 2 A method for detecting milk immunoglobulin (IgG) content in milk

[0035] The capillary electrophoresis temperature was controlled at 30°C, using a silica capillary column with a diameter of 75μm and a length of 600mm, a DAD detector, an injection pressure of 0.5psi, an injection time of 5s, and a working voltage of 25kV.

[0036] Formulation of the calibration curve: use milk immunoglobulin (IgG) standard to prepare standard solutions with concentrations of 2 mg / ml, 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, and 0.1 mg / ml. After shaking, use high-pressure capillary electrophoresis analysis. The integrated peak areas are 15413, 76214, 37502, 18305, 8923. The standard curve is obtained by software, the linear regression correlation coefficient is 0.999, the lowest detection line is 0.01ug, and the lowest detection The output content is 0.01mg / ml, and the recovery rate of the standard sample is 92%.

[0037] Sample buffer configuration method: 160m mol / L trimethylaminomethane ...

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Abstract

The invention relates to a method for detecting components in milk, in particular to a detection method for detecting the content of milk immunoglobulin (IgG), and belongs to the detection technology field. The detection method for detecting the content of the milk immunoglobulin (IgG) through a capillary electrophoresis instrument of the invention comprises the steps of sample pretreatment and sample loading detection, reading calibration, and report editing, etc. In the invention, the electrophoresis of a sample runs on the capillary electrophoresis instrument, and the absorption value of the sample is detected by a capillary chromatographic system and expressed in the form of mapping, thereby the content of the milk immunoglobulin (IgG) can be accurately detected, so as to provide technology support for product development, quality control and component analysis.

Description

technical field [0001] The invention relates to a method for analyzing and detecting protein in milk, in particular to a method for detecting immunoglobulin (IgG) content in milk. It belongs to the technical field of detection. Background technique [0002] Milk is an indispensable nutrient in daily life. It is rich in nutrients, such as casein, whey protein, complement, fat and immunoglobulin. The immunoglobulins contained in milk are mainly IgG, IgA and IgM. Among them, IgG content is the highest, about 80% of the total immunoglobulin. The content of immunoglobulin in the endocrine colostrum of cows on the first day after delivery is the highest. Studies have shown that the average concentration of IgG can reach 59-80mg / ml. However, the IgG concentration in the colostrum on the 5th to 7th day is close to the normal milk level, about 0.7-1.0 mg / ml. Immunoglobulin has a variety of biologically active functions, mainly to identify and resist invading bacteria and viruses ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N30/00
Inventor 孙国庆康小红胡新宇刘卫星
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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