Ultra-high performance liquid chromatography detection method for mannose in honey

An ultra-high performance liquid chromatography and chromatographic detection technology is applied in the field of ultra-high performance liquid chromatography detection of mannose in honey, and achieves the effects of high efficiency, increased pretreatment difficulty and good repeatability

Pending Publication Date: 2021-03-12
武汉市华测检测技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The determination of sugar content generally adopts high performance liquid chromatography-evaporative light scattering detector method or differential detector method, and there is no report on the detection method of mannose content in honey in China.

Method used

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  • Ultra-high performance liquid chromatography detection method for mannose in honey
  • Ultra-high performance liquid chromatography detection method for mannose in honey
  • Ultra-high performance liquid chromatography detection method for mannose in honey

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Embodiment 1

[0041] An ultra-high performance liquid chromatography detection method for mannose in honey, said method comprising:

[0042] 1. Instruments and reagents

[0043] Ultra-high performance liquid chromatography; American Waters-I-class UPLC equipped with evaporative light scattering detector detection;

[0044] Standard substance: mannose, purity 99.6%

[0045] Acetonitrile: chromatographically pure;

[0046] Triethylamine: chromatographically pure;

[0047] The water used in this method is primary water.

[0048]2. Liquid chromatography conditions:

[0049] Column: Waters ACQUITY BEH Amide 1.7μm 2.3×100mm

[0050] Mobile phase: 0.2% ETA acetonitrile solution + water = 84 + 16

[0051] Evaporative photodetector conditions: gain: 200; drift tube temperature: 65°C; carrier gas pressure: 30psi;

[0052] Column temperature: 35°C

[0053] Flow rate: 0.3mL / min

[0054] Injection volume: 3μL

[0055] 3. Linear equation and standard chromatogram

[0056] Standard stock solu...

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Abstract

The invention relates to an ultra-high performance liquid chromatography analysis method for detecting mannose in honey. The method comprises the following steps: performing linear equation and standard chromatogram, performing sample pretreatment, and performing ultra-high performance liquid chromatography condition detection on a pretreated sample. The method can effectively detect the content of mannose in the honey, has good sensitivity, and can identify whether the honey is adulterated according to the content of mannose in the honey.

Description

technical field [0001] The invention belongs to the technical field of food safety, and relates to an ultra-high performance liquid chromatography detection method for mannose in honey. Background technique [0002] Mannose is a reducing monosaccharide. The mannose content in natural honey is extremely low (German QSI believes that mannose does not exist in natural honey), and when the mannose content in honey is detected to exceed 500mg / kg, it is considered to be an illegal additive. The illegal additive is mainly fructose syrup. During the production process, fructose syrup needs to be desalted or impurity-removed through anion-exchange columns. Glucose, fructose and mannose can be transformed into each other through enol structural changes under alkaline conditions. Generally, the mannose content in fructose syrup ranges from several hundred to several thousand, and it is very technically difficult to remove the mannose in fructose syrup during the syrup production proc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/60G01N30/74
CPCG01N30/02G01N30/06G01N30/14G01N30/6052G01N30/74G01N2030/047G01N2030/146G01N2030/067
Inventor 鲍德艳刘翼李媛张晚梅沈蒙田贡洲刘丹丹肖建红王榕李莎
Owner 武汉市华测检测技术有限公司
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