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Fritillaria medicinal material identification and content determination method

A determination method and technology of medicinal materials, applied in the field of quality identification of traditional Chinese medicinal materials, can solve the problems of inability to distinguish between the quantity and quality of the medicinal materials of Fritillaria, and the inability to apply the medicinal materials of Fritillaria, so as to avoid the use of toxic reagents, reduce the cost of reagents, and improve extraction. rate effect

Active Publication Date: 2015-03-11
ZHEJIANG CONBA PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Furthermore, as we all know, Fritillaria zhejiang, Fritillaria sichuan, and Fritillaria chinensis, each medicinal material has a different composition and content of alkaloids in it, so it will use its own high-performance liquid chromatography to detect its own composition and content. These methods It cannot be applied to the research of Fritillaria medicinal materials of the same genus and different species, and it is impossible to make a good quantitative and qualitative distinction between different species of Fritillaria medicinal materials

Method used

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  • Fritillaria medicinal material identification and content determination method
  • Fritillaria medicinal material identification and content determination method

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Embodiment 1

[0053] Example 1: Identification and differentiation of different unknown Fritillaria medicinal materials

[0054] On March 6, 2014, three batches of unknown Fritillaria medicinal materials were purchased from a Chinese pharmacy in Hangzhou, and they were distinguished and judged according to the identification method provided by the present invention:

[0055] (1) Preparation of reference substance solution: Accurately weigh an appropriate amount of reference substance of Peiminin A and Peiminin B, dissolve it in methanol, and use it as a reference substance solution.

[0056] (2) Preparation of the test solution: Get 2g of the above-mentioned three batches of unknown Fritillaria medicinal materials respectively, grind them into powder, put them in a flask, add 200ml 70% ethanol, extract for 3h, filter, reclaim the solvent from the filtrate to obtain a residue, and use 5ml methanol for the residue Dissolve, load the sample on 6g neutral alumina (100-200 mesh), then elute with...

Embodiment 2

[0063] Embodiment 2: Determination of the content of peiminin A and peiminin B in different Fritillaria medicinal materials

[0064] (1) Carry out content determination according to the method of Pharmacopoeia:

[0065] Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel as filler; acetonitrile-water-diethylamine (70:30:0.03) as mobile phase; detection by evaporative light scattering detector. The number of theoretical plates should not be less than 2000 based on the peak of peiminine A.

[0066] Preparation of reference substance solution: take appropriate amounts of peiminin A reference substance and peiminin B reference substance, accurately weighed, add methanol to make a mixed solution containing 0.2 mg of peiminin A and 0.15 mg of peiminin B in every 1 ml , that is.

[0067] Preparation of the test solution: Weigh about 2 g of the powder (passed through a No. 4 sieve, 65 mesh) of Fritillaria japonica, Fritillaria chinensis, and Fri...

Embodiment 3

[0075] Embodiment 3: the investigation of extraction solvent

[0076] Method: Take 2 g of Fritillaria herb powder and put it in a flask, add (or not add) 4.0 mL of concentrated ammonia water to infiltrate for 1 hour, then use 200 ml of chloroform-methanol (4:1), chloroform, methanol, 90% ethanol, 70% ethanol, With 50% ethanol as extraction solvents, reflux extraction for 5 hours, filter, measure the content of peiminin A and peiminin B in the filtrate, and compare the characteristic spectra.

[0077] Result: The results of content determination are shown in Table 2, and the chromatograms measured with chloroform-methanol (4:1) as the extraction solvent are shown in Table 2. Figure 7 , the chromatogram measured with 70% ethanol as the extraction solvent is shown in Figure 8 .

[0078] The influence of table 2 extraction solvent on peimin A, peimin B

[0079]

[0080]

[0081] It can be seen that:

[0082] ① When "do not infiltrate with concentrated ammonia water, on...

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Abstract

The invention discloses a fritillaria medicinal material identification and content determination method comprising the steps: after extracting with ethanol, passing through a neutral alumina column, determining by high performance liquid chromatography, combining with specific chromatograms of medicinal materials, effectively identifying thunberg fritillary bulb, bulbus fritillariae cirrhosae and bulbus fritillariae ussuriensis, and determining the contents of peimine and peiminine. The method not only can effectively identify authenticity of the three fritillaria medicinal materials, but also can accurately determine for the contents of peimine and peiminine, achieves multiple evaluations through one-time determination, and is safe, environmentally friendly, convenient and quick.

Description

technical field [0001] The invention relates to the technical field of quality identification of Chinese medicinal materials, in particular to a method for identification and content determination of Fritillaria medicinal materials. Background technique [0002] Fritillaria fritillaria belongs to the genus Fritillaria of Liliaceae, and the dried bulbs of Fritillaria fritillaria are listed as one of the famous "Eight Flavors of Zhejiang" as one of the precious Chinese herbal medicines. The effect of reducing swelling. Chuan Fritillaria is a genus of Liliaceae Fritillaria. The dried bulbs of Chuan Fritillaria have the effects of clearing away heat and nourishing the lungs, reducing phlegm and relieving cough. It is also a good medicine for blindly treating chronic cough, phlegm and asthma. Flat Fritillaria belongs to the genus Fritillaria of Liliaceae, and the dried bulbs of Flat Fritillaria also have the effects of clearing heat and moistening lungs, relieving cough and red...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 何厚洪叶剑锋闵会陈玲芳方玲吴华铃陈琳乔洪翔胡江宁
Owner ZHEJIANG CONBA PHARMA
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